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The map label for this gene is uxaA

Identifier: 30064434

GI number: 30064434

Start: 3220915

End: 3222402

Strand: Reverse

Name: uxaA

Synonym: S3338

Alternate gene names: 30064434

Gene position: 3222402-3220915 (Counterclockwise)

Preceding gene: 30064435

Following gene: 30064433

Centisome position: 70.06

GC content: 54.23

Gene sequence:

>1488_bases
ATGCAATACATCAAGATCCATGCGCTGGATAACGTCGCGGTCGCTTTAGCAGATTTGGCTGAAGGCACAGAAGTCAGTGT
CGATAACCAGACTGTTACGCTGCGCCAGGATGTTGCTCGTGGACATAAATTTGCGTTAACGGATATCGCAAAAGGGGCCA
ACGTCATCAAATATGGCCTGCCGATTGGTTATGCATTGGCGGATATTGCGGCGGGGGAACACGTCCACGCCCACAATACG
CGCACGAATCTGAGCGATCTGGATCAGTATCGCTATCAACTTGATTTTCAGGATCTGCCTGCGCAAGCGGCAGATCCTGA
AGTGCAGATCTATCGTCGCGCTAACGGCGATGTCGGGGTGCGTAACGAGCTGTGGATCCTGCCAACCGTGGGCTGTGTCA
ACGGCATCGCGCGGCAAATCCAGAACCGTTTCCTGAAAGAGACCAACAACGCCGAAGGTACCGACGGCGTGTTCCTCTTC
AGCCACACCTATGGCTGCTCACAACTGGGCGACGATCACATCAACACCCGCACCATGCTGCAAAACATGGTGCGCCACCC
GAACGCGGGCGCAGTGCTGGTGATTGGTCTGGGCTGTGAAAACAACCAGGTTGCCGCATTCCGTGAAACGTTGGGCGATA
TCGATCCTGAACGCGTTCATTTCATGATCTGCCAACAGCAGGATGATGAGATCGAAGCCGGGATCGAGCATTTGCATCAG
CTGTATAACGTGATGCGCAACGATAAACGCGAGCCAGGCAAACTCAGCGAACTGAAGTTTGGTCTGGAGTGCGGTGGTTC
TGACGGTCTTTCTGGTATTACTGCTAACCCGATGCTGGGGCGTTTCTCTGACTACGTGATTGCTAACGGCGGTACTACCG
TACTGACCGAAGTGCCGGAGATGTTTGGCGCAGAGCAGTTGCTGATGGACCATTGCCGCGACGAAGCAACGTTTGAAAAA
CTGGTCACCATGGTCAACGACTTCAAACAGTACTTTATTGCCCATGATCAGCCGATCTACGAAAACCCATCACCGGGGAA
CAAAGCGGGCGGTATCACCACGCTGGAAGACAAATCACTTGGCTGTACCCAGAAAGCGGGTTCCAGTGTCGTGGTTGACG
TGCTGCGTTACGGCGAGCGTCTGAAAACGCCAGGGCTGAACTTGTTAAGTGCGCCGGGTAACGATGCCGTAGCGACCAGC
GCCCTGGCGGGTGCGGGCTGCCATATGGTGCTGTTCAGTACTGGTCGCGGCACGCCATACGGTGGATTTGTGCCGACGGT
GAAAATCGCCACCAACAGTGAACTGGCGGCGAAGAAAAAACACTGGATCGACTTTGACGCGGGTCAGCTGATCCACGGTA
AAGCGATGCCGCAGTTGCTGGAAGAATTTATCGACACCATCGTTGAGTTTGCCAACGGTAAGCAAACCTGCAACGAGCGT
AACGACTTCCGCGAACTGGCGATCTTCAAAAGCGGCGTAACGCTATAA

Upstream 100 bases:

>100_bases
CGGCGGACATCAACCTGCTGGGCGAGATGGTGAAAAATATTTGCTTTAACAATGCGCGTGACTACTTCGCCATTGAACTG
AACTAAGGTCTGGGGTTGAT

Downstream 100 bases:

>100_bases
AGTCGAAATGAGTTGTTAATAAAAAACGGCGTTTCATAATGTGAAGCGCCGTTTGCTTTTCCATACCTGCTAAGGATTTA
TCATGACCGCGTATTGGCTG

Product: altronate hydrolase

Products: NA

Alternate protein names: Altronic acid hydratase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQLDFQDLPAQAADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL

Sequences:

>Translated_495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQLDFQDLPAQAADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL
>Mature_495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQLDFQDLPAQAADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789475, Length=495, Percent_Identity=99.3939393939394, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI1789516, Length=522, Percent_Identity=31.8007662835249, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.7 [H]

Molecular weight: Translated: 54081; Mature: 54081

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGL
CCEEEEEECCHHHHHHHHHCCCCEEEECCCEEEEHHHHHCCCEEEEHHHHCCCCEEEECC
PIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQAADPEVQIYRRANGDVGV
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHEECHHHCCCCCCCCCEEEEEECCCCCCC
RNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLFSHTYGCSQLGDDHINTRTML
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHH
QNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPE
HHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHH
MFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSL
HHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTPY
CCHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCC
GGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
CCCCCEEEEECCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NDFRELAIFKSGVTL
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGL
CCEEEEEECCHHHHHHHHHCCCCEEEECCCEEEEHHHHHCCCEEEEHHHHCCCCEEEECC
PIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQAADPEVQIYRRANGDVGV
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHEECHHHCCCCCCCCCEEEEEECCCCCCC
RNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLFSHTYGCSQLGDDHINTRTML
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHH
QNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPE
HHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHH
MFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSL
HHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTPY
CCHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCC
GGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
CCCCCEEEEECCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NDFRELAIFKSGVTL
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3038546 [H]