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The map label for this gene is hypA

Identifier: 30064346

GI number: 30064346

Start: 3124078

End: 3124419

Strand: Reverse

Name: hypA

Synonym: S3239

Alternate gene names: 30064346

Gene position: 3124419-3124078 (Counterclockwise)

Preceding gene: 30064347

Following gene: 30064345

Centisome position: 67.93

GC content: 55.56

Gene sequence:

>342_bases
ATGCATGAGTTGTCGCTTTGCCAGAGCGCCGTTGAAATTATCCAACGGCAGGCGGAGCAGCACGATGTTAAGCGCGTCAC
CGCCGTGTGGCTGGAAATTGGCGCGCTCTCCTGCGTTGAGGAGAGCGCCGTCCGTTTTAGTTTTGAAATTGTCTGCCACG
GAACGGTGGCGCAAGGGTGCGATTTACATATCGTCTATAAACCCGCCCAGGCCTGGTGCTGGGATTGCAGCCAGGTGGTG
GAGATTCATCAGCACGATGCGCAGTGTCCGCTCTGTCACGGCGAGCGGTTGCGTGTCGATACCGGCGATTCGCTGATCGT
CAAAAGTATTGAAGTTGAATAA

Upstream 100 bases:

>100_bases
GATGACTGCGCACGAATGATCCTTTCGTTGCCAGTCACCAATCCGGATGTACCACACGCAGGGCGTCGCGCCCTGCTGTT
TGGCCGCAGGAGGGGCGAAA

Downstream 100 bases:

>100_bases
GACTGAGGACAACGCCTAAAAAGCGCACGGACGGTCCCCTCGCCCCTCCGGGGAGAGGGTTAGGGTGAGAGGGAACACCC
GCTAAAGGTTCTTTTTCGCG

Product: hydrogenase nickel incorporation protein HybF

Products: NA

Alternate protein names: Hydrogenase-2 operon protein hybF [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Sequences:

>Translated_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
>Mature_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step [H]

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family [H]

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=100, Blast_Score=227, Evalue=1e-61,
Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=48.2456140350877, Blast_Score=117, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000688
- InterPro:   IPR020538 [H]

Pfam domain/function: PF01155 HypA [H]

EC number: NA

Molecular weight: Translated: 12698; Mature: 12698

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]