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The map label for this gene is hybG

Identifier: 30064345

GI number: 30064345

Start: 3123696

End: 3123944

Strand: Reverse

Name: hybG

Synonym: S3238

Alternate gene names: 30064345

Gene position: 3123944-3123696 (Counterclockwise)

Preceding gene: 30064346

Following gene: 30064343

Centisome position: 67.92

GC content: 53.41

Gene sequence:

>249_bases
ATGTGTATTGGCGTTCCAGGCCAGGTGCTGGCTGTCGGTGAAGATATTCACCAGCTTGCGCAGGTTGAAGTATGTGGTAT
CAAGCGCGACGTGAATATCGCCCTGATTTGCGAAGGTAACCCTGCCGATCTGCTGGGCCAGTGGGTGCTGGTACACGTCG
GATTTGCCATGAGCATCATCGACGAAGATGAAGCCAAAGCCACATTAGACGCACTGCGCCAAATGGATTACGACATTACC
AGCGCGTGA

Upstream 100 bases:

>100_bases
GTCCCCTCGCCCCTCCGGGGAGAGGGTTAGGGTGAGAGGGAACACCCGCTAAAGGTTCTTTTTCGCGTTTGTCCGGCAAT
CGAAATAACCGGAGTTAACA

Downstream 100 bases:

>100_bases
TGATTAGCTTTTGTAGGCCTGATAAGACGCGGCAGCGTCGCATCAGGCATTGTGCACGATTGCCGGATGCGGCGTGAACG
CCTTATCCGGCCTGGATGGC

Product: hydrogenase 2 accessory protein HypG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 82; Mature: 82

Protein sequence:

>82_residues
MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDIT
SA

Sequences:

>Translated_82_residues
MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDIT
SA
>Mature_82_residues
MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDIT
SA

Specific function: May have a specific role in the maturation of the large subunits of HYD1 and HYD2

COG id: COG0298

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupF/hypC family

Homologues:

Organism=Escherichia coli, GI1789364, Length=82, Percent_Identity=100, Blast_Score=166, Evalue=2e-43,
Organism=Escherichia coli, GI1789083, Length=81, Percent_Identity=56.7901234567901, Blast_Score=84, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBG_ECO57 (P0AAM9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85958
- PIR:   C91113
- RefSeq:   NP_289568.1
- RefSeq:   NP_311902.1
- ProteinModelPortal:   P0AAM9
- SMR:   P0AAM9
- EnsemblBacteria:   EBESCT00000025392
- EnsemblBacteria:   EBESCT00000055875
- GeneID:   916301
- GeneID:   958474
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4344
- KEGG:   ecs:ECs3875
- GeneTree:   EBGT00050000009481
- HOGENOM:   HBG556935
- OMA:   NISLICT
- ProtClustDB:   PRK10413
- BioCyc:   ECOL83334:ECS3875-MONOMER
- InterPro:   IPR019812
- InterPro:   IPR001109
- PRINTS:   PR00445
- TIGRFAMs:   TIGR00074

Pfam domain/function: PF01455 HupF_HypC

EC number: NA

Molecular weight: Translated: 8808; Mature: 8808

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS01097 HUPF_HYPC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSII
CEECCCCCEEEECHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEC
DEDEAKATLDALRQMDYDITSA
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSII
CEECCCCCEEEECHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEC
DEDEAKATLDALRQMDYDITSA
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796