Click here to switch to the map view.

The map label for this gene is gsp

Identifier: 30064343

GI number: 30064343

Start: 3120643

End: 3122502

Strand: Reverse

Name: gsp

Synonym: S3236

Alternate gene names: 30064343

Gene position: 3122502-3120643 (Counterclockwise)

Preceding gene: 30064345

Following gene: 30064342

Centisome position: 67.89

GC content: 52.26

Gene sequence:

>1860_bases
ATGAGCAAAGGAACGACCAGCCAGGATGCCCCGTTCGGGACATTATTGGGCTACGCCCCAGGTGGGGTAGCAATCTACTC
TTCAGATTACAGTTCTCTCGATCCGCAGGAATACGAAGATGACGCCGTATTCCGTAGCTATATCGACGACGAATATATGG
GCCACAAGTGGCAATGCGTTGAATTTGCTCGCCGTTTTCTCTTTCTGAATTACGGTGTGGTCTTTACTGACGTGGGTATG
GCGTGGGAGATTTTCTCGCTGCGCTTCCTGCGAGAAGTGGTTAATGACAACATCCTGCCATTGCAGGCATTTCCTAACGG
CTCGCCGCGTGCGCCGGTCGCGGGTGCGCTTCTTATCTGGGATAAAGGCGGTGAATTTAAAGACACTGGCCATGTCGCCA
TCATTACCCAATTGCATGGCAACAAAGTCCGTATTGCGGAACAGAACGTGATTCATTCCCCGTTGCCGCAAGGGCAACAG
TGGACGCGCGAGCTGGAGATGGTGGTCGAAAACGGCTGCTATACCCTTAAAGACACTTTTGATGACACCACCATTCTGGG
CTGGATGATCCAGACGGAAGATACTGAATACAGCTTACCGCAGCCGGAAATTGCAGGCGAGCTGCTGAAAATCAGTGGAG
CGCGTCTGGAAAACAAAGGCCAGTTTGACGGTAAATGGCTGGATGAAAAAGATCCGCTACAAAACGCCTATGTGCAGGCC
AACGGTCAGGTGATCAATCAGGATCCGTATCATTACTACACCATTACCGAGAGTGCCGAGCAGGAGCTAATTAAAGCCAC
CAACGAGCTGCATCTGATGTATCTTCACGCAACCGACAAGGTGCTGAAAGATGACAACCTGCTGGCGCTGTTCGACATTC
CGAAAATCCTCTGGCCACGTTTGCGTCTCTCCTGGCAGCGTCGCCGTCACCATATGATCACTGGTCGTATGGATTTCTGC
ATGGATGAGCGTGGCCTGAAGGTTTACGAATACAACGCCGACTCCGCCTCCTGTCATACCGAAGCGGGCTTGATCCTCGA
ACGTTGGGCGGAGCAGGGCTATAAAGGCAACGGCTTCAATCCGGCGGAAGGGCTGATTAACGAACTGGCTGGTGCCTGGA
AACACAGTCGTGCACGTCCGTTTGTCCATATCATGCAGGACAAAGATATCGAGGAAAACTATCACGCGCAGTTTATGGAG
CAGGCGCTGCACCAGGCGGGCTTTGAAACGCGTATCTTGCGCGGGCTGGATGAACTGGGCTGGGATGCTGCCGGGCAACT
GATTGATGGGGAAGGGCGACTGGTTAACTGCGTGTGGAAAACCTGGGCGTGGGAAACCGCGTTTGATCAGATTCGTGAAG
TTAGCGACCGTGAGTTTGCTGCGGTGCCAATCCGTACCGGTCATCCGCAAAACGAAGTGCGTCTTATCGACGTATTGCTG
CGCCCGGAAGTGCTGGTCTTTGAGCCGCTGTGGACGGTGATCCCCGGCAACAAAGCGATTCTGCCGATCCTCTGGTCGCT
GTTCCCGCACCATCGTTACCTGCTGGATACCGATTTCACTGTTAATGATGAATTGGTGAAAACAGGTTACGCAGTGAAAC
CGATCGCCGGTCGCTGTGGTAGCAATATCGACCTGGTCAGCCATCATGAAGAGGTGCTGGACAAAACCAGCGGTAAATTT
GCCGAGCAGAAAAACATCTATCAGCAACTGTGGTGTTTGCCGAAAGTGGACGGTAAATACATTCAGGTATGTACCTTCAC
CGTTGGCGGCAACTACGGTGGGACGTGTTTGCGCGGTGATGAATCACTGGTCATCAAAAAAGAGAGTGATATTGAACCGT
TAATTGTGGTGAAAAAGTAA

Upstream 100 bases:

>100_bases
TATGATTAGTCTGACCTCTGTCCGGTTAATGTCGCTGAGCAGGTAATCAGAGCACAACAATTACGCCAACTGCTTAGGTT
GTATTGTTAAAGGTAAAGTG

Downstream 100 bases:

>100_bases
TACATGTTGTAGCAACCCCGTACTGATTATGAAGAATAATTAGTACGGGGATTAATCAAAATATTCAATATTTATATATT
CCAATATTTATTCATTTCAT

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFME
QALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKF
AEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK

Sequences:

>Translated_619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFME
QALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKF
AEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK
>Mature_618_residues
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
WEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQW
TRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQAN
GQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQ
ALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFA
EQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain

Homologues:

Organism=Escherichia coli, GI1789361, Length=619, Percent_Identity=100, Blast_Score=1289, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=391, Percent_Identity=27.3657289002558, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1790629, Length=374, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSP_ECOLI (P0AES0)

Other databases:

- EMBL:   U23148
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A57538
- RefSeq:   AP_003543.1
- RefSeq:   NP_417462.1
- PDB:   2IO7
- PDB:   2IO8
- PDB:   2IO9
- PDB:   2IOA
- PDB:   2IOB
- PDB:   3A2Y
- PDB:   3A2Z
- PDB:   3A30
- PDBsum:   2IO7
- PDBsum:   2IO8
- PDBsum:   2IO9
- PDBsum:   2IOA
- PDBsum:   2IOB
- PDBsum:   3A2Y
- PDBsum:   3A2Z
- PDBsum:   3A30
- ProteinModelPortal:   P0AES0
- SMR:   P0AES0
- DIP:   DIP-36018N
- IntAct:   P0AES0
- MINT:   MINT-1234290
- STRING:   P0AES0
- EnsemblBacteria:   EBESCT00000001855
- EnsemblBacteria:   EBESCT00000015881
- GeneID:   947474
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2956
- KEGG:   eco:b2988
- EchoBASE:   EB2720
- EcoGene:   EG12882
- eggNOG:   COG0754
- GeneTree:   EBGT00050000009387
- HOGENOM:   HBG523203
- OMA:   NKAILPV
- ProtClustDB:   PRK10507
- BioCyc:   EcoCyc:GSP-MONOMER
- Genevestigator:   P0AES0
- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth; SSF52440 PreATP-grasp-like

EC number: =6.3.1.8; =3.5.1.78

Molecular weight: Translated: 70533; Mature: 70401

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
CCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEECCCHHCCC
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
ESLVIKKESDIEPLIVVKK
CEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
CCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEECCCHHCCC
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
ESLVIKKESDIEPLIVVKK
CEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503