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The map label for this gene is endA

Identifier: 30064261

GI number: 30064261

Start: 3020839

End: 3021546

Strand: Direct

Name: endA

Synonym: S3140

Alternate gene names: 30064261

Gene position: 3020839-3021546 (Clockwise)

Preceding gene: 30064260

Following gene: 30064262

Centisome position: 65.68

GC content: 54.52

Gene sequence:

>708_bases
ATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGACGTTGGCCGAAGGTATCAATAG
TTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGATGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACT
GGCAGGGCAAAAAAGGCGTTGTTGATCTGCAATCGTGCGGCTATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTA
GAGTGGGAACACATCGTTCCCGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGC
TAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGCGAGGTGAATGGCGATCGCG
GCAATTTTATGTACAGCCAGTGGAATGGCGGCGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAA
GCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACT
CTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAAC
GCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAA

Upstream 100 bases:

>100_bases
AAATAACCACCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAACTTTCGCTACGTTGCTGGCTC
GTTTTAACACGGAGTAAGTG

Downstream 100 bases:

>100_bases
CCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTA
TGCGTATCCCCCGCATTTAT

Product: DNA-specific endonuclease I

Products: NA

Alternate protein names: Endonuclease I; Endo I [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHIVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS

Sequences:

>Translated_235_residues
MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHIVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
>Mature_235_residues
MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHIVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS

Specific function: Has double-strand break activity [H]

COG id: COG2356

COG function: function code L; Endonuclease I

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endA/nucM nuclease family [H]

Homologues:

Organism=Escherichia coli, GI1789314, Length=235, Percent_Identity=99.1489361702128, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007346 [H]

Pfam domain/function: PF04231 Endonuclease_1 [H]

EC number: =3.1.21.1 [H]

Molecular weight: Translated: 26753; Mature: 26753

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCE
VDLQSCGYQVRKNENRASRVEWEHIVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEECCCCCHHHHCCHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEKAAEPPARARGAIARTYFYMR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHEEEE
DQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
CCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCE
VDLQSCGYQVRKNENRASRVEWEHIVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEECCCCCHHHHCCHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEKAAEPPARARGAIARTYFYMR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHEEEE
DQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
CCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867949; 9278503; 4955462; 8113204 [H]