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The map label for this gene is yggJ

Identifier: 30064262

GI number: 30064262

Start: 3021626

End: 3022357

Strand: Direct

Name: yggJ

Synonym: S3141

Alternate gene names: 30064262

Gene position: 3021626-3022357 (Clockwise)

Preceding gene: 30064261

Following gene: 30064263

Centisome position: 65.7

GC content: 53.01

Gene sequence:

>732_bases
ATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCA
TATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAA
TTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATT
CACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCAT
TACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGA
TTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGT
GCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCTTTACCGGTTGA
ACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTG
ATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGC
GATTTGGGCTAA

Upstream 100 bases:

>100_bases
TGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGA
GACGACGCGGATTTTTAACT

Downstream 100 bases:

>100_bases
CGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAAACATCAACATCAAGAAAGATTCCAGTTTCGC
TATGTTGCTGGAAGCACAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=100, Blast_Score=500, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME_ECOL6 (P0AGL8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755407.2
- ProteinModelPortal:   P0AGL8
- SMR:   P0AGL8
- EnsemblBacteria:   EBESCT00000041209
- GeneID:   1039445
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3532
- GeneTree:   EBGT00050000010165
- HOGENOM:   HBG664092
- OMA:   YLFAPLK
- ProtClustDB:   PRK11713
- GO:   GO:0005737
- InterPro:   IPR015947
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA; SSF88697 PUA-like

EC number: =2.1.1.193

Molecular weight: Translated: 26978; Mature: 26978

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCC
SVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLD
CEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEC
SERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCC
TLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHHCCEEEHHHHHHHHHHHHHEEEEC
DLG
CCC
>Mature Secondary Structure
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCC
SVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLD
CEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEC
SERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCC
TLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHHCCEEEHHHHHHHHHHHHHEEEEC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157