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The map label for this gene is hycF

Identifier: 30064074

GI number: 30064074

Start: 2807586

End: 2807705

Strand: Reverse

Name: hycF

Synonym: S2929

Alternate gene names: 30064074

Gene position: 2807705-2807586 (Counterclockwise)

Preceding gene: 30064075

Following gene: 30064073

Centisome position: 61.05

GC content: 49.17

Gene sequence:

>120_bases
ATGTTTACCTTTATCAAAAAAGTCATCAAAACCGGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGCGCAAGGACA
ATCGGCAAACGGTGCAGTACACTGCGTTGATTGCGTGTAA

Upstream 100 bases:

>100_bases
AGTCGATGTGCGTAAGAAGAAGAGCAAAGTGGTGCCGTACAAAGAACTCGAGCGTTACAGCATTGAGCGTAAAAACTCGC
CGCTGAAATAAGGAATCGCC

Downstream 100 bases:

>100_bases
AGGGATGAAAATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGTTTCAAAAGCGACCGTTTCCCGCGTGCTTT
CAGGTAATGGCTACGTCAGC

Product: iron-sulfur protein of hydrogenase 3 (part of FHL complex)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 39; Mature: 39

Protein sequence:

>39_residues
MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV

Sequences:

>Translated_39_residues
MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV
>Mature_39_residues
MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 4114; Mature: 4114

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA