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The map label for this gene is ascG

Identifier: 30064073

GI number: 30064073

Start: 2806562

End: 2807572

Strand: Reverse

Name: ascG

Synonym: S2928

Alternate gene names: 30064073

Gene position: 2807572-2806562 (Counterclockwise)

Preceding gene: 30064074

Following gene: 30064072

Centisome position: 61.04

GC content: 52.62

Gene sequence:

>1011_bases
ATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGTTTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGGCTA
CGTCAGCCAGGAGACTAAAGATCGCGTGTTTCAGGCGGTAGAAGAGAGCAGTTACCGCCCAAATTTGCTGGCCCGCAATC
TGTCGGCGAAGAGTACTCAGACGCTGGGGCTGGTAGTGACCAACACGCTTTATCACGGCATTTATTTTAGTGAATTACTG
TTTCATGCCGCGCGAATGGCGGAAGAGAAAGGGCGACAGTTGCTATTGGCAGATGGTAAACACAGCGCCGAAGAAGAGCG
CCAGGCGATTCAGTATCTGCTGGATTTGCGCTGCGACGCCATTATGATTTACCCGCGTTTTTTAAGCGTGGATGAGATTG
ATGACATCATTGACGCGCACAGTCAGCCGATAATGGTGCTTAATCGCCGCCTGCGCAATAATAGCAGCCATAGCGTCTGG
TGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGAGTTGATAAACGCCGGGCATCAGGAGATTGCTTTCCTTACCGG
CTCGATGGATTCCCCCACCAGCATTGAACGTCTTGCCGGGTATAAAGACGCGCTGTCGCAGCATGGTATTGCGCTCAATG
AAAAACTTATCGCTAACGGTAAATGGACGCCCGCCAGCGGGGCCGAAGGGGTAGAAACGTTGCTCGAACGAGGGGCTAAA
TTTAGCGCGTTAGTTGCCAGTAACGACGATATGGCGATAGGTGCGATCAAAGCGTTGCACGAGCGCGGCGTAGCGGTGCC
AGAGCAGGTGTCAGTTATCGGATTCGATGATATCGCAATTGCCCCCTATATCGTTCCGGCGCTCTCCAGCGTAAAAATTC
CGGTGACTGAGATGATTCAGGAAATTATTGGACGGCTGATTTTTATGCTCGATGGCGGGGATTTCTCACCGCCGAAAACC
TTTAGCGGAAAACTGATCCGCCGCGACTCACTCATTGCCCTTTCGCGATAA

Upstream 100 bases:

>100_bases
GGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGCGCAAGGACAATCGGCAAACGGTGCAGTACACTGCGTTGATTG
CGTGTAAAGGGATGAAAATG

Downstream 100 bases:

>100_bases
ACATTGTCATCACATGTCATCGTCACTAGTGTCGATGACATCTTTTTTCCTGCATTACAGAAATTAATTTCCTTAAATAA
CAGCAAATTAAAAACTGGCA

Product: ascBF operon repressor

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESSYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELL
FHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPASGAEGVETLLERGAK
FSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKT
FSGKLIRRDSLIALSR

Sequences:

>Translated_336_residues
MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESSYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELL
FHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPASGAEGVETLLERGAK
FSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKT
FSGKLIRRDSLIALSR
>Mature_335_residues
TTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESSYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLF
HAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVWC
DHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPASGAEGVETLLERGAKF
SALVASNDDMAIGAIKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTF
SGKLIRRDSLIALSR

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=97.9166666666667, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.3293413173653, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787948, Length=301, Percent_Identity=30.2325581395349, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.2250803858521, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=28.75, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=27.6872964169381, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=27.9761904761905, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=25.4237288135593, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=284, Percent_Identity=29.5774647887324, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=24.9211356466877, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=26.4026402640264, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=24.9180327868852, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36826; Mature: 36694

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESSYRPNLLARNLSAKSTQ
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC
TLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCE
IMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVWCDHKQTSFNAVAELINAGHQ
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCC
EIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPASGAEGVETLLERGAK
EEEEEECCCCCCHHHHHHHCHHHHHHHCCCEECCCEEECCCCCCCCCCHHHHHHHHCCCC
FSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQ
EEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHH
EIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIALSR
HHHHHHEEEECCCCCCCCCCCCCCEEECCCEEEECC
>Mature Secondary Structure 
TTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESSYRPNLLARNLSAKSTQ
CHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC
TLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCE
IMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVWCDHKQTSFNAVAELINAGHQ
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCC
EIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPASGAEGVETLLERGAK
EEEEEECCCCCCHHHHHHHCHHHHHHHCCCEECCCEEECCCCCCCCCCHHHHHHHHCCCC
FSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQ
EEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHH
EIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIALSR
HHHHHHEEEECCCCCCCCCCCCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]