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The map label for this gene is ygbD

Identifier: 30064070

GI number: 30064070

Start: 2802220

End: 2803353

Strand: Direct

Name: ygbD

Synonym: S2925

Alternate gene names: 30064070

Gene position: 2802220-2803353 (Clockwise)

Preceding gene: 30064069

Following gene: 30064080

Centisome position: 60.93

GC content: 54.59

Gene sequence:

>1134_bases
ATGAGCAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCAC
TATTCCATTAACCCTGATTGCCGCCGACAGCATAGATGAGTACAACAAACCCGACCTCAGCCATGTTATCAGTCAGGGGC
AACGTGCCGACGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGGGCAGTTTAATCTGCGCCTGTTTCCGCACACCTGG
GTGACGGATATCGATGCCGAAGCCCATGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGTTAGTGCTGGCAAC
CGGTGCCAGCGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCT
GTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTCGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTT
TGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGCCAGTATTCTGGCGTCATTAATGCCACCAGAAGTAAGCAG
CCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTGCAGGGGCTGGAAAAAACGGATT
CTGGCATTCTGGCAACGCTGGACCACCAGCGTAGTATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAA
ACAGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATGCCGA
TATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTGCAGCCGATTCAACTTAGCGGGATGG
TGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTACCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTA
CCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAGATTAATACCGAACGCCAGGGAATGGTGGCGCG
CGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGTATTGTTGAAAA
CGTTGCCGGTGTAG

Upstream 100 bases:

>100_bases
TTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAC
GAACTAGCATCGGAGGCAAA

Downstream 100 bases:

>100_bases
GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAT
AAGGCATTTACGCCGCATCC

Product: nitric oxide reductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: Flavorubredoxin reductase; FlRd-reductase; FlavoRb reductase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKPDLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTW
VTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDHQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV

Sequences:

>Translated_377_residues
MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKPDLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTW
VTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDHQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV
>Mature_376_residues
SNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKPDLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWV
TDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFC
RAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDHQRSIEVDAVIAATGLRPET
ALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELP
LHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV

Specific function: One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=315, Percent_Identity=21.9047619047619, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI65787454, Length=321, Percent_Identity=21.4953271028037, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI21389617, Length=321, Percent_Identity=21.4953271028037, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4757732, Length=209, Percent_Identity=26.7942583732057, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI22202629, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789065, Length=377, Percent_Identity=96.816976127321, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=25.077399380805, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788892, Length=274, Percent_Identity=27.3722627737226, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787352, Length=246, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=315, Percent_Identity=24.1269841269841, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639257, Length=373, Percent_Identity=23.5924932975871, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281359715, Length=373, Percent_Identity=23.5924932975871, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281359713, Length=373, Percent_Identity=23.5924932975871, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639250, Length=373, Percent_Identity=23.5924932975871, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18543267, Length=373, Percent_Identity=23.5924932975871, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639252, Length=373, Percent_Identity=23.5924932975871, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585130, Length=280, Percent_Identity=22.1428571428571, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 41228; Mature: 41097

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKPDLSHVISQGQRADDLT
CCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHCCEEEEEECCCCCEECCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDHQRSIEVDAVIAATGLRPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSGMVLAKNLL
HHHHHHCCCEEECCCEEHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCHHCHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
CCCCCEEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEECCCCCHHHHHHHEE
SEDRMKEAFVLLKTLPV
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKPDLSHVISQGQRADDLT
CCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHCCEEEEEECCCCCEECCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDHQRSIEVDAVIAATGLRPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSGMVLAKNLL
HHHHHHCCCEEECCCEEHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCHHCHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
CCCCCEEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEECCCCCHHHHHHHEE
SEDRMKEAFVLLKTLPV
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA