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The map label for this gene is emrB

Identifier: 30064051

GI number: 30064051

Start: 2781149

End: 2782687

Strand: Direct

Name: emrB

Synonym: S2900

Alternate gene names: 30064051

Gene position: 2781149-2782687 (Clockwise)

Preceding gene: 30064050

Following gene: 30064062

Centisome position: 60.47

GC content: 56.08

Gene sequence:

>1539_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCGCAACTGGTCATTATGACGATTGCGCTGTCACTGGCGACATTCATGCAGGT
GCTGAACTCCACCATTGCTAACGTGGCGATCCCCACTATCGCCGGGAATCTGGGCTCATCGCTCAGCCAAGGAACGTGGG
TAATCACTTCTTTCGGGGTGGCGAATGCCATCTCGATCCCGCTTACCGGCTGGCTGGCAAAGCGCGTCGGGGAAGTGAAA
CTGTTCCTTTGGTCCACCATCGCCTTTGCTATTGCGTCTTGGGCGTGTGGTGTCTCCAGCAGCCTGAATATGCTGATCTT
CTTCCGCGTGATTCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAG
CCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTAT
ATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCA
AACCCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCG
GCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGTTTTTCATCACAGGAAATTATCATTCTTACCGTGGTG
GCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTC
GCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGT
TGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATTCTG
TCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTG
CTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTTGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGGTTTG
CGGTGGTCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCGGAACGACTGGCGGCGGCATCGAGC
CTCTCTAACTTTACGCGAACGCTGGCGGGGTCTATCGGCACGTCGATAACCACGACCATGTGGACCAACCGCGAGTCGAT
GCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTG
GGATGACGCAACAGCAGGCATCAGGCTGGATTGCCCAGCAGATCACCAATCAGGGGCTGATTATTTCCGCCAATGAGATC
TTCTGGATGTCAGCCGGGATATTCCTCGTACTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGG
CGGAGGCGGTGCGCACTAA

Upstream 100 bases:

>100_bases
GTCGCGGTAAGTACCGCGCGTGAAATCAGCCTGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGCTAACGCTGG
CTAATCCAGAGGTGCGTGTG

Downstream 100 bases:

>100_bases
GTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCT
GCAACTTCTCTTTCGGCAGT

Product: multidrug resistant protein emrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDCFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Sequences:

>Translated_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDCFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
>Mature_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDCFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=512, Percent_Identity=99.21875, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=61.6302186878728, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=31.924882629108, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=28.960396039604, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=27.0588235294118, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=532, Percent_Identity=22.1804511278195, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=26.8370607028754, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=29.9319727891156, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55615; Mature: 55615

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDCFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCHHHHHHH
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FCASAWPQFIQGFAVVCFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
ECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCH
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDCFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCHHHHHHH
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FCASAWPQFIQGFAVVCFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
ECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCH
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]