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The map label for this gene is yfiC

Identifier: 30063976

GI number: 30063976

Start: 2702686

End: 2703543

Strand: Reverse

Name: yfiC

Synonym: S2810

Alternate gene names: 30063976

Gene position: 2703543-2702686 (Counterclockwise)

Preceding gene: 30063978

Following gene: 30063975

Centisome position: 58.78

GC content: 52.56

Gene sequence:

>858_bases
GTGTGGGGCGCTGATTATAGACGTTACGGCTGCAATCTTCATCTGTTTGTATGGATATCGCTTCTCGGGTATTGTTCTCA
CCCGGTGCTATCGCCGTTTTTCCTGCAAGGTTTTATTTTCATGTCACAGTCTACATCCGTGCTTCGTCGTAATGGATTTA
CTTTTAAACAGTTTTTTGTTGCTCACGATCGCTGTGCGATGAAAGTGGGAACGGATGGCATTTTGCTGGGCGCATGGGCA
CCGGTGGCTGGGGTGAAACGTTGCCTTGATATCGGCGCGGGTAGCGGGTTGCTGGCACTGATGCTGGCGCAGCGAACCAG
TGACAGCGTGATAATTGATGCCGTTGAGCTGGAAAGTGAAGCTGCCGCGCAGGCGCAGGAAAATATCAACCAGTCCCCGT
GGGCAGAGCGGATTAACGTCCATACGGCGGATATTCAGCAGTGGATCACACAGCAGACAGTACGCTTCGATTTAATCATC
AGTAACCCACCTTACTATCAGCAGGGAGTAGAGTGCGCGACACCTCAACGGGAACAGGCTCGCTATACCACTACGCTTGA
TCACCCATCGCTGCTGACCTGTGCGGCGGAGTGCATTACCGAAGAGGGATTTTTCTGCGTGGTGCTGCCAGAGCAGATTG
GTAATGGTTTTACTGAACTGGCATTAAGCATGGGCTGGCATTTACGTTTGCGGACGGATGTGGCGGAAAACGAAGCGCGA
CTGCCGCATCGGGTGCTGCTGGCATTCTCCCCGCAGGCGGGAGAATGCTTTAGCGATCGCTTAGTCATTCGTGGGCCAGA
CCAGAACTATTCCGAAGCGTATACGGCGCTGACCCAGGCTTTTTATCTGTTTATGTAA

Upstream 100 bases:

>100_bases
ATGGCGGTCGGGCGAGTGAAACCTTTATCCTGGAGGGCTTCCAGCAGGCTTTCGTCGAGTTCAAGTTCGGAAAAAGTCGT
TACAGTCATGTTCTACCTCT

Downstream 100 bases:

>100_bases
TGATTGCCGGGGGAAAGGATCGATGGACCGGAATGCGTGAGCAGTTCCGGGTAATCCAGCGTAAAATGCAGCCCCCGGCT
TTCTTTGCGCATCATCGCGC

Product: putative enzyme

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MWGADYRRYGCNLHLFVWISLLGYCSHPVLSPFFLQGFIFMSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWA
PVAGVKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEAR
LPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM

Sequences:

>Translated_285_residues
MWGADYRRYGCNLHLFVWISLLGYCSHPVLSPFFLQGFIFMSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWA
PVAGVKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEAR
LPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM
>Mature_285_residues
MWGADYRRYGCNLHLFVWISLLGYCSHPVLSPFFLQGFIFMSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWA
PVAGVKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEAR
LPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val) [H]

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082131, Length=245, Percent_Identity=98.7755102040816, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007848
- InterPro:   IPR022882 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.55 [H]

Molecular weight: Translated: 31946; Mature: 31946

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGADYRRYGCNLHLFVWISLLGYCSHPVLSPFFLQGFIFMSQSTSVLRRNGFTFKQFFV
CCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEECCCHHHHHHCCCHHHHHHH
AHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESE
HHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEHHHHH
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQA
HHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCCCCCHHHC
RYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEAR
CEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHCCCEEEEECCCCCCCCC
LPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM
CCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWGADYRRYGCNLHLFVWISLLGYCSHPVLSPFFLQGFIFMSQSTSVLRRNGFTFKQFFV
CCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEECCCHHHHHHCCCHHHHHHH
AHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESE
HHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEHHHHH
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQA
HHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCCCCCHHHC
RYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEAR
CEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHCCCEEEEECCCCCCCCC
LPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM
CCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA