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The map label for this gene is yfiE

Identifier: 30063978

GI number: 30063978

Start: 2704924

End: 2705850

Strand: Reverse

Name: yfiE

Synonym: S2812

Alternate gene names: 30063978

Gene position: 2705850-2704924 (Counterclockwise)

Preceding gene: 30063980

Following gene: 30063976

Centisome position: 58.83

GC content: 48.11

Gene sequence:

>927_bases
ATGTTTTTATATTCTAAATTCGATATTTTCGATAGGTTTGGGGTTATGGATCTGCGCCGTTTTATTACGCTTAAAACCGT
GGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTGTACACAATCGACGGTGACTTTTCATATTCAGCAGC
TTGAGCAGGAGTTTTCAGTCCAGTTATTTGAGAAAATTGGCCGACGGATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTG
CCGCACATTTATGAACTTACCCGAGTGATGGATACGCTTCGTGAGGCTGCCAAAAAGGAGTCAGATCCGGACGGGGAACT
ACGCGTTGTTTCGGGCGAAACGTTGCTCTCTTACCGAATGCCGCAGGTGTTGCAGCGTTTTCGGCAACGCGCACCAAAAG
TGCGTTTATCGTTGCAGGCGCTGAACTGTTACGTGATCCGTGATGCGCTACTGAATGATGAGGCCGATGTCGGCGTCTTT
TATCGCGTAGGGAATGATGATGCCCTGAACCGACGAGAGTTGGGTGAACAATCGCTGGTGCTGGTGGCTTCACCACAAAT
TGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAGCTTTATTATCAACGAACCGCAATGTGTCTTCCGGC
AGATATTTGAGAGCACGCTGCGACAGCGGCGGATCACGGTGGAAAACACCATTGAGCTTATAAGTATCGAAAGCATCAAG
CGTTGTGTCGCGGCGAATATCGGTGTCAGTTACTTGCCCCACTTTGCGGTGGAAAAAGAGATTGAGAGCGGAGAATTAAT
TGAATTGCCTTTTGGCGAACAGTCGCAGACCATTACGGCAATGTGCGCACATTATGCTGGAAAAGCGGTTAGCCCGGCGA
TGCACATTTTTATTCAGTGTGTTGAAGAGTGTTTTGTGGTTGCGTAA

Upstream 100 bases:

>100_bases
TCAGGGTGTAAGTCCAAAAAGCACTTAAAAGGATCGGTGTCACTTGTTATCTCCTCTGTTTTGGTAGACAGTACCCGACA
CGGAACATTTAAAAAAATGA

Downstream 100 bases:

>100_bases
AAAAACAATGCCGGGCTTAAACCCGGCATTGAGATTACTCTTCTGTCGTTTGCGGTGGCACGCCCGTTCCGCTCGGTTTA
CGGCGCTTACCAATATTTTT

Product: putative LYSR-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFLYSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIK
RCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSPAMHIFIQCVEECFVVA

Sequences:

>Translated_308_residues
MFLYSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIK
RCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSPAMHIFIQCVEECFVVA
>Mature_308_residues
MFLYSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIK
RCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSPAMHIFIQCVEECFVVA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=96.5870307167236, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=25.1700680272109, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.0418118466899, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=27.1565495207668, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=21.8106995884774, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=23.3576642335766, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788706, Length=274, Percent_Identity=25.1824817518248, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35214; Mature: 35214

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLYSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHHHHHEEHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHEEHHHHH
RCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSPAMHIFIQC
HHHHHCCCHHHCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
VEECFVVA
HHHHHHCC
>Mature Secondary Structure
MFLYSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHHHHHEEHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHEEHHHHH
RCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSPAMHIFIQC
HHHHHCCCHHHCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
VEECFVVA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]