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The map label for this gene is wcaK

Identifier: 30063496

GI number: 30063496

Start: 2105618

End: 2106898

Strand: Reverse

Name: wcaK

Synonym: S2231

Alternate gene names: 30063496

Gene position: 2106898-2105618 (Counterclockwise)

Preceding gene: 30063497

Following gene: 30063495

Centisome position: 45.81

GC content: 54.25

Gene sequence:

>1281_bases
ATGAAATTACTTATTCTGGGCAACCACACTTGCGGCAATCGTGGCGACAGCGCCATCTTGCGCGGCTTACTTGATGCCAT
CAACATCCTCAATCCACATACCGAAGTGGACGTGATGAGCCGCTATCCGGTCAGTTCTTCCTGGCTGCTCAACCGCCCGG
TAATGGGCGATCCGCTGTTCCTGCAGATGACACAACACAATAGCGCGGCGGGCGTTGTCGGGCGCATTAAAAAAGTCCTC
CGTCGCCGCTACCAGCATCAGGTATTGCTCTCACGCGTCACCGACACTGGCAAGCTGCGCAATATCGCCATCGCCCAGGG
ATTCACCGACTTCGTGCGCCTGCTGTCAGGTTACGACGCCATTATTCAGGTCGGCGGATCGTTTTTTGTCGATCTCTACG
GCGTGCCGCAGTTTGAACATGCGCTTTGCACATTTATGGCGAAAAAGCCGCTGTTTATGATTGGTCACAGCGTCGGCCCC
TTCCAGGATGAGCAATTTAACCAACTGGCGAACTATGTCTTTGGTCACTGCGACGCGCTGATCCTGCGCGAATCGGTCAG
CCTTGATTTGATGAAACGCAGAAATATCACCACCGCAAAAGTGGAACATGGCGTCGATACCGCGTGGCTGGTCGATCACC
ACACCGAAGACTTCACCGCCAGCTATGCCGTTCAACACTGGCTGGACGTTGCCGCACAACAGAAAACAGTGGCAATTACC
CTGCGCGAACTGGCACCGTTCGACAAACGTCTCGGCACCACTCAACAAGCGTATGAAAAAGCCTTTGCCGGGGTGGTCAA
TCGCATTCTCGATGAAGGCTATCAGGTGATTGCGCTCTCCACCTGTACGGGTATCGACAGCTATAACAAAGACGACCGCA
TGGTGGCGCTCAATCTGCGCCAGCACATCAGCGATCCTGCCCGTTACCACGTAGTGATGGATGAACTCAACGATCTGGAA
ATGGGCAAAATTCTCGGAGCCTGTGAACTCACCGTCGGTACGCGCCTGCACTCCGCCATTATCTCAATGAACTTTGCTAC
TCCGGCGATTGCCATCAACTATGAACATAAATCCGCCGGGATTATGCAGCAGCTGGGACTGCCAGAGATGGCGATTGATA
TTCGTCATTTACTGGACGGCAGCCTGCAAGCGATGGTTGCGGATACCTTAGGCCAGCTTCCGGTGCTGAACGCTCGATTT
AACGAAGCCGTCAGTCGCGAACGTCAGACGGGAATGCAGATGGTGCAGTCTGTGCTTGAGCGCATCGGGGAGGTGAAATG
A

Upstream 100 bases:

>100_bases
CCTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAAAAACGGCAACGAACACAACAAACACAGCCTCACCTGGC
ATCAAATTTATGAGGAAAAA

Downstream 100 bases:

>100_bases
AGGTCGGCTTCTTCTTATTGAAATTCCCGCTGTCGTCGGAAACCTTCGTCCTCAATCAAATTACCGCGTTTATTGATATG
GGCTTTGAAGTGGAGATTGT

Product: putative pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPVSSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARF
NEAVSRERQTGMQMVQSVLERIGEVK

Sequences:

>Translated_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPVSSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARF
NEAVSRERQTGMQMVQSVLERIGEVK
>Mature_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPVSSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARF
NEAVSRERQTGMQMVQSVLERIGEVK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=426, Percent_Identity=98.1220657276995, Blast_Score=871, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47485; Mature: 47485

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCCEE
LQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARFNEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC
>Mature Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCCEE
LQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARFNEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]