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The map label for this gene is wcaL

Identifier: 30063495

GI number: 30063495

Start: 2104401

End: 2105621

Strand: Reverse

Name: wcaL

Synonym: S2230

Alternate gene names: 30063495

Gene position: 2105621-2104401 (Counterclockwise)

Preceding gene: 30063496

Following gene: 30063494

Centisome position: 45.78

GC content: 54.22

Gene sequence:

>1221_bases
ATGAAGGTCGGCTTCTTCTTATTGAAATTCCCGCTGTCGTCGGAAACCTTCGTCCTCAATCAAATTACCGCGTTTATTGA
TATGGGCTTTGAAGTGGAGATTGTCGCTTTGCAAAAAGGCGATACAGAGAACACCCACGCGGCATGGACGAAATACAACC
TTGTCGCCAGAACCCGCTGGTTACAGGACGAACCTGCGGGCAAAGTAGCGAAACTGCGCCACCGCGCCAGCCAGACCTTA
CGTGGCATTCATCGTAAAAATACCTGGCATGCGCTCAACCTCAAACGCTATGGTGCCGAGTCGCGGAACCTGATTTTGTC
TGCCATTTGTGGTCAGGTCGCAACACCGTTTCGCGCCGATGTGTTCATCGCTCATTTTGGCCCGGCGGGGGTAACCGCAG
CAAAACTACGCGAACTGGGTATCATTCGCGGCAAAATTGCCACCATCTTCCACGGTATTGATATCTCCAGTCGGGAAGTG
CTCAACCACTACACTCCCGAATATCAACAACTGTTTCGCCGTGGCGACCTGATGTTACCGATAAGCGATCTGTGGGCCGG
AAGGCTGCAAAAAATGGGCTGCCCCAGAGAAAAAATCGCCGTATCGCGTATGGGCGTGGACATGACGCGCTTTAGCCCGC
GTCCGGTGAAAGCGCCCGCAACGCCGCTGGAAATCATATCTGTCGCACGCTTAACCGAGAAAAAAGGCCTGCATGTGGCG
ATTGAAGCCTGCCGTCAGTTGAAAGAGCAGGGCGTGGCATTTCGCTATCGCATCCTCGGCATTGGCCTGTGGGAACGACG
CCTGCGCACCATCATCGAACAATATCAACTGGAAGATGTGGTAGAGATGCCGGGCTTTAAACCGAGCCATGAAGTGAAGG
AGATGCTCGACGACGCGGATGTCTTCCTGTTGCCATCGGTAACGGGCGCGGATGGCGATATGGAAGGCATTCCGGTGGCG
CTAATGGAAGCGATGGCGGTCGGTATTCCGGTGGTTTCTACTCTGCATAGCGGAATTCCTGAACTGGTGGAGGCCGATAA
ATCCGGCTGGCTGCTGCCTGAGAACGATGCTCGCGCACTGGCACAACGTCTGGCGGCGTTTAGCCAACTGGATACCGACG
AACTGGCTCCGGTCGTCAAACGTGCGTGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCC
AGCTTGCTGCAGGCTTTATAG

Upstream 100 bases:

>100_bases
TTCCGGTGCTGAACGCTCGATTTAACGAAGCCGTCAGTCGCGAACGTCAGACGGGAATGCAGATGGTGCAGTCTGTGCTT
GAGCGCATCGGGGAGGTGAA

Downstream 100 bases:

>100_bases
AGGAGAACGTATGCCATTTAAAACACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCC
CTTTCGCTCGCGCACTTCCC

Product: putative colanic acid biosynthesis glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTENTHAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTL
RGIHRKNTWHALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQLEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQQVINRELA
SLLQAL

Sequences:

>Translated_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTENTHAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTL
RGIHRKNTWHALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQLEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQQVINRELA
SLLQAL
>Mature_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTENTHAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTL
RGIHRKNTWHALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQLEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQQVINRELA
SLLQAL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=97.2906403940887, Blast_Score=793, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45405; Mature: 45405

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTENTHAAWTKYNLVARTRW
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESRNLILSAICGQVATPFRAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHHHHCCCCCCC
VFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQLEDVVEMPGFKPSHEVKEMLDDAD
HHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLLPENDARAL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRACEKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTENTHAAWTKYNLVARTRW
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESRNLILSAICGQVATPFRAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHHHHCCCCCCC
VFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQLEDVVEMPGFKPSHEVKEMLDDAD
HHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLLPENDARAL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRACEKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]