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The map label for this gene is rstB

Identifier: 30063124

GI number: 30063124

Start: 1698900

End: 1700201

Strand: Direct

Name: rstB

Synonym: S1763

Alternate gene names: 30063124

Gene position: 1698900-1700201 (Clockwise)

Preceding gene: 30063123

Following gene: 30063125

Centisome position: 36.94

GC content: 50.31

Gene sequence:

>1302_bases
ATGAAAAAATTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTTGGGCTGGTGTA
CAAATTTACCGCCGAACGCGCGGGCAAACAGTCGCTGGATGATTTGATGAACAGTTCGCTGTATCTGATGCGCAGCGAAT
TGCGAGAGATCCCCCCACACGACTGGGGTAAGACGCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAG
CCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTA
CACGTTTTTGCAGCGTATCCCGCGCAGCCACTACGTGCTGGCAGTTGGTCCTGTTCCTTATCTTTATTACCTCCATCAAA
TGCGATTGCTGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCAC
TGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAACGTATCCACTTTGATGA
GGGTTCGAGCTTTGAACGACTTGGTATCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAAC
AGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTG
AGCGCCGCCGAATCCCAGGCGTTGAATCGTGATATCAGTCAACTTGAAGCTTTAATTGAAGAGCTGCTGACTTATGCCCG
ACTCGATCGCCCACAAAACGAGCTTCATCTTAGCGAACCAGACCTGCCGTTGTGGCTGTCAACGCATCTGGCAGATATTC
AGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCATGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATG
GAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGCTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGG
GAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTG
TTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCAATAGCACTGACA
ATGGGCGGTACGGTTAATTGTGACATCAGCGAACTGGGTGGTGCTCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACAT
CCCGCAATTTACCTCTGCCTGA

Upstream 100 bases:

>100_bases
CCGGACATTATCTTCCAAACAAGGTCTTATCCAGTTGGCGGTTTACATTGCTCCACAGTCGTGAATTAATACGAATAGTA
TTCTGTTGCGGGCGATGCCA

Downstream 100 bases:

>100_bases
CACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGC
GCGTTACGATCTCGTAGACC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALT
MGGTVNCDISELGGARFSFSWPLWHNIPQFTSA

Sequences:

>Translated_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALT
MGGTVNCDISELGGARFSFSWPLWHNIPQFTSA
>Mature_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALT
MGGTVNCDISELGGARFSFSWPLWHNIPQFTSA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=99.0762124711316, Blast_Score=883, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=34.5588235294118, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=32.0754716981132, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786783, Length=311, Percent_Identity=29.2604501607717, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=31.140350877193, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789403, Length=457, Percent_Identity=26.6958424507659, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=23.7918215613383, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787374, Length=283, Percent_Identity=26.8551236749117, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=23.6220472440945, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=28.3464566929134, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=278, Percent_Identity=28.7769784172662, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=27.7533039647577, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=248, Percent_Identity=26.2096774193548, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=24.3589743589744, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790551, Length=213, Percent_Identity=29.5774647887324, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=26.8722466960352, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49372; Mature: 49372

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLMQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALTMGGTVNCDISELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC
>Mature Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLMQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALTMGGTVNCDISELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]