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The map label for this gene is ordL

Identifier: 30062821

GI number: 30062821

Start: 1358244

End: 1359524

Strand: Direct

Name: ordL

Synonym: S1388

Alternate gene names: 30062821

Gene position: 1358244-1359524 (Clockwise)

Preceding gene: 30062820

Following gene: 30062822

Centisome position: 29.53

GC content: 57.69

Gene sequence:

>1281_bases
ATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGCGAATAAATATGCACCATTCGACACACTGAATGAGTCAATCAC
CTGCGACGTTTGCGTGGTTGGCGGCGGCTATACCGGGCTCTCCTCCGCGCTGCATCTGGCGGAAGCGGGCTTTGACGTAG
TGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGGCAGCTTGTGAACTCCTACAGCCGCGAC
ATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAGGGTGGCGAGATCAT
CCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGTCTGTTTGTGGCGATGAATGATAAACAAC
TCGCCACACTCGAAGAGCAGAAAGAGAACTGGGTACGCTACGGCAATAAACAGCTGGAGCTGCTGGACGTCAACGCTATT
CGCCGCGAGGTCGCCAGCGATCGCTACACTGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCACCCGCTAAACCTCGC
CATTGGTGAAGCCGATGCCATCCGCCTGAACGGCGGGCGCGTGTATGAGCTTTCCGCCGTGACGCAGATCCAGCACACCA
CGCCAGCCGTGGTTCGGACTGCCAATGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGAT
AAAGTAGAGCCGGAACTAGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTT
AGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACA
ACCGCCTGCTGTACGGCGGGGGTGTAGTGTATGGTGCACGCAACCCGGATGACGTTGAGCGTCTTGTGGTACCGAAACTA
CTAAAAACCTTCCCTCAGTTGAAGGGCGTGAAAATTGATTACCGCTGGACGGGCAACTTCCTGCTGACCTTGTCGCACAT
GCCGCAGTTCGGCCGCCTTGATACCAACATCTATTACATGCAGGGTTACAGCGGCCACGGCGTCACCTGTACCCATCTTG
CCGGGCGTTTGATTGCTGAACTGCTGCGCGGTGACGCCGAGCGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTC
CCCGGCGGGCGCACGCTGCGCGTGCCATTTACCGCGATGGGCGCGGCTTATTACAGCCTGCGCGATCGTCTGGGCGTTTA
A

Upstream 100 bases:

>100_bases
GGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACTGAACTGAAAACCATCTG
GATAAGCCTGGAGGCCTGAA

Downstream 100 bases:

>100_bases
TTTCCGATTAACAGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCTACCC
CACGCGGGGTTGGCGTGATG

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV

Sequences:

>Translated_426_residues
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV
>Mature_425_residues
TEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDI
DVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAIR
REVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGDK
VEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLL
KTFPQLKGVKIDYRWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFP
GGRTLRVPFTAMGAAYYSLRDRLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=98.8262910798122, Blast_Score=868, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47134; Mature: 47003

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCCEEEECCCCCCCHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEEEEEECCEECCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSHMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRLGV
HHHHCC
>Mature Secondary Structure 
TEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCEEEECCCCCCCHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEEEEEECCEECCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSHMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]