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The map label for this gene is insA [H]

Identifier: 30062132

GI number: 30062132

Start: 619450

End: 619734

Strand: Reverse

Name: insA [H]

Synonym: S0607

Alternate gene names: 30062132

Gene position: 619734-619450 (Counterclockwise)

Preceding gene: 30062133

Following gene: 30062131

Centisome position: 13.47

GC content: 52.98

Gene sequence:

>285_bases
GTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACCGGCAAAAGCAC
TGCCGGACATCAGCGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCG
GTACGCACCAGAAAATCATTGATATGGCCATGAATGACGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTC
AACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAA

Upstream 100 bases:

>100_bases
TATTAAGAATAATTAATTGTTAACATATCTAATCCAATAAGATGTTATGTTGTGGTAATGCTGCCAACTTACTGATTTAG
TGTATGATGGTGATTTTAAG

Downstream 100 bases:

>100_bases
CCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGTGCTAAATCA
CGTCAGCGCTGGCTGTTTTA

Product: IS1 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MLAWLPFPSDVLPAPLLKAWCVTGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNDVGCRASARIMGVGL
NTVLRHLKNSGRSR

Sequences:

>Translated_94_residues
MLAWLPFPSDVLPAPLLKAWCVTGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNDVGCRASARIMGVGL
NTVLRHLKNSGRSR
>Mature_94_residues
MLAWLPFPSDVLPAPLLKAWCVTGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNDVGCRASARIMGVGL
NTVLRHLKNSGRSR

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=71, Percent_Identity=95.7746478873239, Blast_Score=146, Evalue=2e-37,
Organism=Escherichia coli, GI1788204, Length=71, Percent_Identity=95.7746478873239, Blast_Score=146, Evalue=2e-37,
Organism=Escherichia coli, GI1786204, Length=71, Percent_Identity=95.7746478873239, Blast_Score=146, Evalue=2e-37,
Organism=Escherichia coli, GI87081814, Length=71, Percent_Identity=94.3661971830986, Blast_Score=145, Evalue=5e-37,
Organism=Escherichia coli, GI1786471, Length=71, Percent_Identity=94.3661971830986, Blast_Score=145, Evalue=5e-37,
Organism=Escherichia coli, GI1786460, Length=71, Percent_Identity=94.3661971830986, Blast_Score=145, Evalue=5e-37,
Organism=Escherichia coli, GI1790747, Length=72, Percent_Identity=94.4444444444444, Blast_Score=142, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10483; Mature: 10483

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAWLPFPSDVLPAPLLKAWCVTGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKII
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHH
DMAMNDVGCRASARIMGVGLNTVLRHLKNSGRSR
HHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAWLPFPSDVLPAPLLKAWCVTGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKII
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHH
DMAMNDVGCRASARIMGVGLNTVLRHLKNSGRSR
HHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]