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The map label for this gene is rna

Identifier: 30062069

GI number: 30062069

Start: 545935

End: 546759

Strand: Reverse

Name: rna

Synonym: S0535

Alternate gene names: 30062069

Gene position: 546759-545935 (Counterclockwise)

Preceding gene: 30062070

Following gene: 30062068

Centisome position: 11.89

GC content: 50.91

Gene sequence:

>825_bases
ATGAGTTCCACACCCATTATGAAAGCATTCTGCCGTAACGCCGCGTTGCTCGCGGTTTCTCTGCTTCCCTTATCTTCTGC
CAACGCCGTAGCGTTGCAGGCAAAACAGTATGGCGATTTTGATCGCTATGTCCTGGCCCTCTCCTGGCAAACCGGATTTT
GCCAGAGTCAACACGATCGAAATCGTAACGAACGAGATGAATGTCGCCTGCAAACCGAAACGACCAACAAAACTGATTTT
CTGACCGTACATGGTCTGTGGCCAGGATTGCCTAAATCGGTTGCTGCCCGTGGTGTTGATGAACGCCGCTGGATGCGCTT
CGGTTGCGCTACTCGCCCAATCCCGAATCTACCAGAAGCACGCGCCAGCCGAATGTGTTCATCGCCGGAAACCGGGTTAT
CACTGGAAACGGCCGCTAAACTAAGTGAAGTCATGCCAGGAGCTGGCGGACGTTCCTGCCTGGAACGCTACGAATATGCC
AAACACGGTGCCTGCTTTGGTTTTGATCCGGACGCATACTTCGGTACGATGGTACGCCTGAATCAAGAAATTAAAGAGAG
CGAAGCCGGAAAATTCCTTGCGGATAATTACGGTAAAACAGTGAGCCGCCGTGACTTTGACGCCGCCTTTGCCAAAAGCT
GGGGAAAAGAGAACGTGAAAGCAGTTAAGCTAACGTGCCAGGGTAACCCTGCGTATTTGACTGAAATTCAGATCTCGATC
AAAGCTGACGCCATCAACGCTCCGCTTTCTGCAAACTCATTCTTGCCACAACCTCACCCAGGTAACTGTGGCAAAACCTT
TGTGATTGATAAAGCGGGTTATTAA

Upstream 100 bases:

>100_bases
ACTAATACGTTCGCCAAACGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAAT
AGATAGTATTCAGTGTATGT

Downstream 100 bases:

>100_bases
TCCTCTGATTTACAAAACACGCTAATTCGTCTTCCATAACCATGCCGATTAAGAATTATACATAATGCGCTGCATTGAGG
AATTCCCTGAATGTGACGCA

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSSTPIMKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKTDF
LTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYA
KHGACFGFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISI
KADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY

Sequences:

>Translated_274_residues
MSSTPIMKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKTDF
LTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYA
KHGACFGFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISI
KADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY
>Mature_273_residues
SSTPIMKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKTDFL
TVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAK
HGACFGFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIK
ADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=268, Percent_Identity=98.5074626865672, Blast_Score=553, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 30133; Mature: 30002

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPIMKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDR
CCCCHHHHHHHCCHHEEEHEEECCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHC
NRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEA
CCCCCHHHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHH
RASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFGTMVRL
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISI
HHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEE
KADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY
EECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
SSTPIMKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDR
CCCHHHHHHHCCHHEEEHEEECCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHC
NRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEA
CCCCCHHHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHH
RASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFGTMVRL
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISI
HHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEE
KADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY
EECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]