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The map label for this gene is gtrBI

Identifier: 30061871

GI number: 30061871

Start: 322766

End: 323695

Strand: Reverse

Name: gtrBI

Synonym: S0320

Alternate gene names: 30061871

Gene position: 323695-322766 (Counterclockwise)

Preceding gene: 30061872

Following gene: 30061870

Centisome position: 7.04

GC content: 42.26

Gene sequence:

>930_bases
ATGAAAATATCTCTTGTCGTTCCTGTCTTCAATGAAGAAGAAGCGATACCTGTTTTCTATAAAACGGTACGTGAATTCCA
AGAGTTGAAGCCATATGAAGTAGAAATTGTATTCATAAATGACGGAAGTAAAGATGCCACAGAGTCAATTATTAACGCGC
TGGCTGTTTCAGACCCGCTAGTTGTTCCGCTGTCATTTACACGCAACTTTGGTAAAGAACCAGCCTTATTTGCAGGGTTA
GACCATGCAAGCGGCGATGCTGTAATTCCTATTGATGTCGACCTGCAAGACCCAATTGAGGTTATCCCTCATCTTATTGA
AAAGTGGCAGGCAGGTGCTGACATGGTGCTTGCTAAACGTTCAGACCGCTCAACTGATGGACGACTGAAACGTAAGACAG
CTGAGTGGTTCTATAAATTACACAACAAAATAAGCACCCCAAAGATCGAGGAAAATGTCGGAGATTTTCGACTCATGTCT
CGTGAGGTTGTGGAGAACATTAAACTGTTGCCTGAGCGCAATCTTTTCATGAAAGGCATACTGAGCTGGGTGGGTGGTCA
GACGGATGTCGTTGAATATGTACGCGCAGAGCGTGTTGCTGGCATCTCAAAATTTAATGGCTGGAAATTATGGAATCTGG
CACTGGAAGGTATCACAAGTTTTTCAACCTTTCCTCTTCGCGTATGGACTTATATAGGCTTGTTTGTTGCAAGCATTTCA
TTTTTATATGGTGCATGGATGATTATAGACACCCTTGTCTTTGGTAACCCAGTACGCGGGTATCCCTCCCTGCTTGTATC
AATACTTTTCTTGGGTGGAGTGCAACTGATCGGGATTGGTGTTCTCGGAGAATATATAGGTAGAATCTATATTGAAGTAA
AAAATAGACCCAAATACATCATAAAAAAATCTCATCGAGGTAACCCATGA

Upstream 100 bases:

>100_bases
AATGCTCACTTCCTCCAATAGTCACTCTTATCACCTTCTCCGCCATCAGTCTGGTGTGCGGTTTCGTCTATTCAAAGTTC
ATTGTCTTTAGGGATGCGAA

Downstream 100 bases:

>100_bases
TTAAAATTAATTTATTTAAAAATGCAAATCTCCTTGCCTTCATATCATGTTTTGCAATATCAATTTATTGTTATTGGGGG
TGGTTGTACGATGGAACACT

Product: putative bactoprenol glucosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSKDATESIINALAVSDPLVVPLSFTRNFGKEPALFAGL
DHASGDAVIPIDVDLQDPIEVIPHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIGLFVASIS
FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP

Sequences:

>Translated_309_residues
MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSKDATESIINALAVSDPLVVPLSFTRNFGKEPALFAGL
DHASGDAVIPIDVDLQDPIEVIPHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIGLFVASIS
FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP
>Mature_309_residues
MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSKDATESIINALAVSDPLVVPLSFTRNFGKEPALFAGL
DHASGDAVIPIDVDLQDPIEVIPHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIGLFVASIS
FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP

Specific function: Involved in O antigen modification. Catalyzes the transfer of the glucose residue from UDP-glucose to a lipid carrier

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family. GtrB subfamily

Homologues:

Organism=Escherichia coli, GI1788692, Length=306, Percent_Identity=89.8692810457516, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1788588, Length=309, Percent_Identity=32.3624595469256, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GTRB_SHIFL (P68667)

Other databases:

- EMBL:   AE005674
- EMBL:   AE014073
- RefSeq:   NP_706258.1
- RefSeq:   NP_836042.1
- ProteinModelPortal:   P68667
- SMR:   P68667
- EnsemblBacteria:   EBESCT00000085784
- EnsemblBacteria:   EBESCT00000090174
- GeneID:   1025741
- GeneID:   1076753
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF0306
- KEGG:   sfx:S0320
- GeneTree:   EBGT00050000010589
- HOGENOM:   HBG752660
- OMA:   FVASISF
- ProtClustDB:   CLSK891776
- BioCyc:   SFLE198214:AAN41965.1-MONOMER
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: 2.4.1.- [C]

Molecular weight: Translated: 34847; Mature: 34847

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf916948)-; HASH(0x103ef234)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSKDATESIINALAVSDPL
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCE
VVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVIPHLIEKWQAGADMVLAKR
EEEEHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
SDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMSREVVENIKLLPERNLFMKGI
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIGLFVASIS
HHHHCCCHHHHHHHHHHHHHCCHHCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCCCCEE
IKKSHRGNP
EECCCCCCH
>Mature Secondary Structure
MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSKDATESIINALAVSDPL
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCE
VVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVIPHLIEKWQAGADMVLAKR
EEEEHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
SDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMSREVVENIKLLPERNLFMKGI
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIGLFVASIS
HHHHCCCHHHHHHHHHHHHHCCHHCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRPKYI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCCCCEE
IKKSHRGNP
EECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152