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The map label for this gene is yadP

Identifier: 30061707

GI number: 30061707

Start: 152770

End: 153309

Strand: Reverse

Name: yadP

Synonym: S0142

Alternate gene names: 30061707

Gene position: 153309-152770 (Counterclockwise)

Preceding gene: 30061714

Following gene: 30061706

Centisome position: 3.33

GC content: 55.93

Gene sequence:

>540_bases
GTGCCTCACATGTCTGAACCGCAACGTCTGTTCTTTGCTATCGACTTACCTGCAGAAATCCGCGAACAGATTATCCATTG
GCGCGCCACACACTTCCCACCTGAGGCGGGACGTCCGGTCGCCGCCGATAATTTGCACCTGACTCTGGCATTTTTAGGCG
AAGTGAGCGCAGAGAAAGAGAAGGCGCTTTCTCTTTTAGCCGGACGGATTCGTCAACCTGGTTTCACACTCACGCTTGAT
GACGCCGGACAATGGCTGCGTTCGCGTGTGGTGTGGTTAGGGATGCGTCAGCCTCCACGCGGCTTAATCCAGCTGGCGAA
TATGCTCCGTTCACAGGCTGCCCGCAGCGGTTGTTTTCAAAGCAATCGTCCGTTTCATCCACATATTACCTTATTGCGCG
ACGCCAGCGAGGCGGTGACATTCCCGCCGCCGGGTTTTAACTGGTCTTATGCGGTGACGGAGTTCACCCTTTACGCCTCC
TCGTTTGCCCGTGGACGCACACGCTACACGCCGCTAAAACGCTGGGCGCTAACGCAATAA

Upstream 100 bases:

>100_bases
TTCAGGTAAGACGGCAGCAACGGGCAACGACGACACAAAACGCTCCAGAGGGTTAACATTCTTCGCGCCACATTGTAGCA
TCGCGGTAATTCATAACCGA

Downstream 100 bases:

>100_bases
CAAGGATTGTCGCAATGGAATTTTCTCCCCCTCTACAGCGCGCGACGCTAATTCAGCGTTACAAACGTTTTTTAGCCGAT
GTGATCACACCCGATGGTCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPHMSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLD
DAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYAS
SFARGRTRYTPLKRWALTQ

Sequences:

>Translated_179_residues
MPHMSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLD
DAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYAS
SFARGRTRYTPLKRWALTQ
>Mature_178_residues
PHMSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDD
AGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASS
FARGRTRYTPLKRWALTQ

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=99.4318181818182, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20334; Mature: 20202

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHMSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKE
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHH
KALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQ
HHHHHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCC
SNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
PHMSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKE
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHH
KALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQ
HHHHHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCC
SNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]