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The map label for this gene is hemL

Identifier: 30061714

GI number: 30061714

Start: 164748

End: 166028

Strand: Reverse

Name: hemL

Synonym: S0149

Alternate gene names: 30061714

Gene position: 166028-164748 (Counterclockwise)

Preceding gene: 30061717

Following gene: 30061707

Centisome position: 3.61

GC content: 55.74

Gene sequence:

>1281_bases
ATGAGTAAGTCTGAAAATCTTTACAGCGCAGCGCGCGAGCTGATCCCTGGCGGTGTGAACTCCCCTGTTCGCGCCTTTAC
TGGCGTGGGCGGCACTCCACTGTTTATCGAAAAAGCGGACGGCGCTTATCTGTACGATGTTGATGGCAAAGCCTATATCG
ATTATGTCGGTTCCTGGGGGCCGATGGTGCTGGGCCATAACCATCCGGCGATCCGCAATGCCGTGATTGAAGCCGCCGAG
CGTGGTTTAAGCTTTGGTGCACCGACCGAAATGGAAGTGAAAATGGCGCAACTGGTGACCGAACTGGTCCCGACCATGGA
TATGGTGCGCATGGTGAACTCCGGCACCGAGGCGACGATGAGCGCCATCCGCCTGGCCCGTGGTTTTACCGGTCGCGACA
AAATCATTAAATTTGAAGGTTGTTACCACGGTCACGCTGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCACTCACGTTA
GGCCAGCCAAACTCGCCGGGCGTTCCGGCAGATTTCGCCAAACATACCTTAACCTGTACTTATAACGATCTGGCCTCTGT
ACGCGCCGCGTTTGAGCAATACCCGCAAGAGATTGCCTGTATTATCGTCGAGCCGGTGGCAGGCAATATGAACTGTGTTC
CGCCGCTGCCAGAGTTCCTGCCAGGTCTGCGTGCGCTGTGCGACGAATTTGGCGCGTTGCTGATCATCGATGAAGTGATG
ACCGGTTTCCGCGTAGCGCTAGCTGGCGCACAGGATTATTTCGCCGTAGTGCCAGATTTAACCTGCCTCGGAAAAATCAT
CGGCGGTGGAATGCCGGTAGGCGCATTCGGTGGTCGTCGTGATGTAATGGATGCGCTGGCCCCGACAGGTCCGGTCTATC
AGGCGGGTACGCTTTCCGGTAACCCGATTGCGATGGCAGCGGGTTTCGCCTGTCTGAATGAAGTCGCGCAGCCGGGCATT
CACGAAACGCTGGATGAGCTGACAACACGTCTGGCAGAAGGTCTGCTGGAAGCGGCAGAAGAAGCGGGAATTCCGCTGGT
CGTTAACCACGTCGGTGGTATGTTCGGTATCTTCTTTACCGACGCCGAGTCCGTGACGTGCTATCAGGATGTGATGGCCT
GTGACGTCGAGCGTTTTAAGCGTTTCTTCCATATGATGCTGGATGAAGGTGTTTACCTGGCACCGTCAGCGTTTGAAGCA
GGCTTTATGTCTGTGGCGCACAGCATGGAAGATATCAATAACACCATCGATGCTGCACGTCGGGTGTTCGCGAAGTTGTG
A

Upstream 100 bases:

>100_bases
TGCACCAGTACAAGCAGCCTGATGTTTGACGAGTATTTAACTTGTTATGAATAACATGGAATAGCAGCCATTCACTTTTT
CTACGAATCAGGAACCCTCC

Downstream 100 bases:

>100_bases
TAATTTTGCCTCGCCCACCGGGCGAGGCATTCTATTAACGGCTCTGCTTTCTCAGCAAATAGATAAAATATGGCGCACCG
ATAAAGGTCGACAGCAGCCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGI
HETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL

Sequences:

>Translated_426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGI
HETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL
>Mature_425_residues
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAER
GLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLG
QPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVMT
GFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIH
ETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAG
FMSVAHSMEDINNTIDAARRVFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=315, Percent_Identity=29.8412698412698, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI13994255, Length=376, Percent_Identity=27.1276595744681, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI284507298, Length=230, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=99.0610328638498, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=447, Percent_Identity=29.9776286353468, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=32.0809248554913, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=32.3717948717949, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=30.9148264984227, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI145693181, Length=334, Percent_Identity=29.6407185628743, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786991, Length=384, Percent_Identity=25.78125, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=305, Percent_Identity=28.1967213114754, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=364, Percent_Identity=28.021978021978, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=329, Percent_Identity=25.2279635258359, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=298, Percent_Identity=30.5369127516779, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=26.9349845201238, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357415, Length=301, Percent_Identity=27.2425249169435, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356575, Length=325, Percent_Identity=28, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45353; Mature: 45221

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGIHETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC
>Mature Secondary Structure 
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGIHETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA