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The map label for this gene is ygaA

Identifier: 161486446

GI number: 161486446

Start: 2799083

End: 2800597

Strand: Reverse

Name: ygaA

Synonym: S2923

Alternate gene names: 161486446

Gene position: 2800597-2799083 (Counterclockwise)

Preceding gene: 30064071

Following gene: 30064061

Centisome position: 60.89

GC content: 56.77

Gene sequence:

>1515_bases
ATGAGTTTTTCCGTTGATGTGCTGGCGAATATCGCCATCGAATTGCAGCGTGGGATTGGTCATCAGGATCGTTTTCAGCG
CCTGATCACCACGCTACGTCAGGTGCTGGAGTGCGATGCGTCTGCGTTGCTACGTTACGATTCGCGGCAGTTTATTCCGC
TTGCCATCGACGGTCTGGCAAAGGATGTACTCGGTAGACGCTTTGCGCTGGAAGGGCATCCACGGCTGGAAGCGATTGCC
CGCGCCGGGGATGTGGTGCGCTTTCCCGCAGACAGCGAATTGCCCGATCCCTATGACGGTTTGATTCCTGGGCAGGAGAG
TCTGAAGGTTCACGCCTGCGTTGGTCTGCCATTGTTTGTCGGACAAAACCTGATCGGCGCACTGACGCTCGACGGGATGC
AGCCCGATCAGTTCGATGTTTTCAGCGACGAAGAGTTACGGCTGATTGCCGCGCTGGCGGCGGGAGCGTTAAGCAATGCG
TTGCTGATTGAACAACTGGAAAGCCAGAATATGCTGCCAGGCGATGCCACGCCGTTTGAAGCGGTGAAACAGACGCAGAT
GATTGGCTTGTCCCCTGGCATGACGCAACTGAAAAAAGAGATTGAGATTGTGGCGGCGTCCGATCTCAACGTTCTGATCA
GCGGTGAGACGGGAACCGGTAAGGAGCTGGTGGCGAAAGCGATTCATGAAGCCTCGCCACGGGCGGTGAATCCGCTGGTC
TATCTCAACTGTGCCGCACTGCCGGAAAGTGTGGCGGAAAGTGAGTTGTTCGGGCATGTGAAAGGAGCGTTTACTGGCGC
TATCAGTAACCGCAGCGGGAAGTTTGAAATGGCGGATAACGGTACTCTGTTTCTCGATGAGATCGGCGAGTTGTCGTTGG
CATTGCAGGCCAAGCTGCTGAGGGTGTTGCAGTATGGCGATATTCAGCGCGTTGGCGATGACCGTAGTTTGCGGGTCGAT
GTGCGCGTGCTGGCGGCGACTAACCGCGACTTACGCGAAGAGGTGCTGGCAGGGCGATTCCGCGCCGATTTGTTTCATCG
CCTGAGCGTGTTTCCACTTTCGGTGCCGCCGCTGCGTGAGCGGGGCGATGATGTCATTCTGCTGGCGGGGTATTTCTGCG
AGCAGTGTCGTTTGCGGCAGGGGCTCTCCCGCGTGGTATTAAGTGCCGGGGCGCGAAATTTACTGCAACACTACAGTTTT
CCGGGAAACGTGCGCGAACTGGAACATGCTATTCATCGGGCGGTAGTGCTGGCGAGAGCCACCCGCAACGGCGATGAAGT
GATTCTTGAGGCGCAACATTTTGCTTTTCCTGAGGTGACGTTGCCGCCGCCAGAAGCGGCGGCGGTGCCCGTTGTTAAGC
AAAACCTGCGTGAAGCGACAGAAGCGTTCCAGCGTGAAACTATTCGCCAGGCACTGGCACAAAATCATCACAACTGGGCA
GCCTGTGCGCGAATGCTGGAAACTGACGTCGCCAACCTGCATCGGCTGGCGAAACGTCTGGGATTGAAGGATTAA

Upstream 100 bases:

>100_bases
TCTATAAATAAAATTATGCTCATTAATGAGTAGTCAAAATGACTATCAATCAAATGGTCGATATGACAATATCTATAGTC
AAATTGACAGTGAGGCAAAG

Downstream 100 bases:

>100_bases
ATAATCCCGGCCTGATAGAAATCCTGCAGGTTAATTGCGCCGGTGAGTTTGCCGTTTTCATCTACCACTGGTGCGGCAGT
GATTTTGCGTTTCATCAGAA

Product: anaerobic nitric oxide reductase transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIA
RAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGARNLLQHYSF
PGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
ACARMLETDVANLHRLAKRLGLKD

Sequences:

>Translated_504_residues
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIA
RAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGARNLLQHYSF
PGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
ACARMLETDVANLHRLAKRLGLKD
>Mature_503_residues
SFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIAR
AGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNAL
LIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLVY
LNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDV
RVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGARNLLQHYSFP
GNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWAA
CARMLETDVANLHRLAKRLGLKD

Specific function: Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082152, Length=504, Percent_Identity=99.0079365079365, Blast_Score=1004, Evalue=0.0,
Organism=Escherichia coli, GI1789087, Length=363, Percent_Identity=40.771349862259, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI87082117, Length=318, Percent_Identity=47.1698113207547, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788550, Length=299, Percent_Identity=46.1538461538462, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1788905, Length=316, Percent_Identity=39.873417721519, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1790299, Length=336, Percent_Identity=40.4761904761905, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=38.4384384384384, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=38.3435582822086, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=338, Percent_Identity=38.1656804733728, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1786524, Length=246, Percent_Identity=45.9349593495935, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=36.9294605809129, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI87081858, Length=220, Percent_Identity=34.0909090909091, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55244; Mature: 55113

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHH
KDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFV
HHHHCCEEECCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
GQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNALLIEQLESQNMLPGDATPFE
CCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
HHHHHHHCCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RGDDVILLAGYFCEQCRLRQGLSRVVLSAGARNLLQHYSFPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
CCCCCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ACARMLETDVANLHRLAKRLGLKD
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHH
KDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFV
HHHHCCEEECCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
GQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNALLIEQLESQNMLPGDATPFE
CCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
HHHHHHHCCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RGDDVILLAGYFCEQCRLRQGLSRVVLSAGARNLLQHYSFPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
CCCCCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ACARMLETDVANLHRLAKRLGLKD
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA