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The map label for this gene is gph [H]

Identifier: 29349592

GI number: 29349592

Start: 5517450

End: 5518100

Strand: Reverse

Name: gph [H]

Synonym: BT_4184

Alternate gene names: 29349592

Gene position: 5518100-5517450 (Counterclockwise)

Preceding gene: 29349593

Following gene: 29349590

Centisome position: 88.14

GC content: 44.09

Gene sequence:

>651_bases
ATGAAGAAACTAGTCATTTTCGATTTGGATGGTACATTACTGAATACCATTGCCGACCTTGCACACAGTACAAATTATGC
CTTGAATAAGTTAGGATATCCCACACATGAGATCGAGAAATATAACTTCATGGTAGGAAACGGCATCGACAAACTTTTTG
AGCGTGCCTTGCCGGAAGGAGAAAAGTCCAAAGAGAATGTACTGCGCGTACGCAAGGAATTCGTCCCTTACTATGACATT
CATAATGCCGATGACAGCCGCCCTTATCCCGGTATTCCGGAATTACTCTCATACTTACAGGATGCAGGTATTCAAATTAC
TGTGGCTTCCAACAAATATCAGGCAGCCACCCAAAAGTTGATTGACCATTATTTTCCGGAAATCCATTTTACCGCTGTGT
TCGGGCAACGTGAAGGGATAAAGGTTAAACCCGATCCAACCGTTGTATTCGATATTCTGGAAGTTGCCAAAGTAACCAAA
GAAGAAGTGCTGTACGTGGGTGATTCCGGCGTGGATATGCAAACGGCTGCTAATGCCGGAGTGACAGTTTGTGGAGTAAC
ATGGGGATTCCGCCCACGTGCCGAACTGGAAGAATTCAGTCCTCAATATATTGTAGATACCGCTGAGGAGATCAAACGTC
TTATTCTCTAA

Upstream 100 bases:

>100_bases
GACTGGTTCCGAATTACAAGGAAATGATTCGTTTTTTATTCCTATCTTTGTAAGCAGAAAGAAAGGAAAACAGGATTAAC
ATAAAAAAACAAGAAGTTCA

Downstream 100 bases:

>100_bases
TATCTGAATCTACAGAAAGATCTCATTCTTCAAGCTGTACGATAGATCTATGCGGATTATATCAACATAGATCTATCATG
CAGCTATTTTTCTTCAATAA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKKLVIFDLDGTLLNTIADLAHSTNYALNKLGYPTHEIEKYNFMVGNGIDKLFERALPEGEKSKENVLRVRKEFVPYYDI
HNADDSRPYPGIPELLSYLQDAGIQITVASNKYQAATQKLIDHYFPEIHFTAVFGQREGIKVKPDPTVVFDILEVAKVTK
EEVLYVGDSGVDMQTAANAGVTVCGVTWGFRPRAELEEFSPQYIVDTAEEIKRLIL

Sequences:

>Translated_216_residues
MKKLVIFDLDGTLLNTIADLAHSTNYALNKLGYPTHEIEKYNFMVGNGIDKLFERALPEGEKSKENVLRVRKEFVPYYDI
HNADDSRPYPGIPELLSYLQDAGIQITVASNKYQAATQKLIDHYFPEIHFTAVFGQREGIKVKPDPTVVFDILEVAKVTK
EEVLYVGDSGVDMQTAANAGVTVCGVTWGFRPRAELEEFSPQYIVDTAEEIKRLIL
>Mature_216_residues
MKKLVIFDLDGTLLNTIADLAHSTNYALNKLGYPTHEIEKYNFMVGNGIDKLFERALPEGEKSKENVLRVRKEFVPYYDI
HNADDSRPYPGIPELLSYLQDAGIQITVASNKYQAATQKLIDHYFPEIHFTAVFGQREGIKVKPDPTVVFDILEVAKVTK
EEVLYVGDSGVDMQTAANAGVTVCGVTWGFRPRAELEEFSPQYIVDTAEEIKRLIL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=205, Percent_Identity=28.780487804878, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24344; Mature: 24344

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVIFDLDGTLLNTIADLAHSTNYALNKLGYPTHEIEKYNFMVGNGIDKLFERALPEG
CCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHEEEEECCCHHHHHHHHCCCC
EKSKENVLRVRKEFVPYYDIHNADDSRPYPGIPELLSYLQDAGIQITVASNKYQAATQKL
CCHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHH
IDHYFPEIHFTAVFGQREGIKVKPDPTVVFDILEVAKVTKEEVLYVGDSGVDMQTAANAG
HHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCC
VTVCGVTWGFRPRAELEEFSPQYIVDTAEEIKRLIL
EEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLVIFDLDGTLLNTIADLAHSTNYALNKLGYPTHEIEKYNFMVGNGIDKLFERALPEG
CCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHEEEEECCCHHHHHHHHCCCC
EKSKENVLRVRKEFVPYYDIHNADDSRPYPGIPELLSYLQDAGIQITVASNKYQAATQKL
CCHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHH
IDHYFPEIHFTAVFGQREGIKVKPDPTVVFDILEVAKVTKEEVLYVGDSGVDMQTAANAG
HHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCC
VTVCGVTWGFRPRAELEEFSPQYIVDTAEEIKRLIL
EEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]