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The map label for this gene is nuoI [H]

Identifier: 29349471

GI number: 29349471

Start: 5296978

End: 5297466

Strand: Reverse

Name: nuoI [H]

Synonym: BT_4063

Alternate gene names: 29349471

Gene position: 5297466-5296978 (Counterclockwise)

Preceding gene: 29349472

Following gene: 29349470

Centisome position: 84.62

GC content: 44.79

Gene sequence:

>489_bases
ATGGAATATAAAGATCAAAAATATACGTATTTGGGAGGACTGATACATGGTATCAGCACTCTGGCAACGGGTATGAAAAC
CAGTATCAAGGTATACTTCCGTAAGAAGGTAACCGAACAGTATCCTGAAAACCGGAAAGAGCTGAAAATGTTCGACCGGT
TCCGTGGTACACTGGCAATGCCCCACAATGAGAATAACGAACACCGCTGTGTAGCCTGCGGATTGTGTCAGATAGCTTGT
CCGAACGATACAATCACCGTAACCAGCGAAACGATCGAGACTGAAGACGGCAAGAAGAAGAAAATCCTGGCAAAATATGA
ATATGACCTCGGAGCTTGTATGTTCTGCCAACTGTGTGTCAACGCTTGTCCGCATGACGCCATCACGTTCGACCAGAACT
TCGAACATGCCGTCTTTGACCGGACGAAGCTTGTTCTGCAACTGAATCATGCCGGTAGTAAAGTAATTGAAAAGAAAAAA
GAAGTTTAG

Upstream 100 bases:

>100_bases
CTGAATCTGGAATGGAAATATCTGGTTCCAATTTCTATGGTAAACCTATTATTAATGGCATGTTGTGTTGCCTTTGGCTT
CCACTTCTAAAATAAGAATT

Downstream 100 bases:

>100_bases
ATATGGGATCAACACTTGAAACAGTAGTATTCTACTTTTTGGCAGCATTTATTATTGCAATGTCCATCATGACGGTGACT
ACCCAACGTATTGTCCGCTC

Product: NADH dehydrogenase I subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MEYKDQKYTYLGGLIHGISTLATGMKTSIKVYFRKKVTEQYPENRKELKMFDRFRGTLAMPHNENNEHRCVACGLCQIAC
PNDTITVTSETIETEDGKKKKILAKYEYDLGACMFCQLCVNACPHDAITFDQNFEHAVFDRTKLVLQLNHAGSKVIEKKK
EV

Sequences:

>Translated_162_residues
MEYKDQKYTYLGGLIHGISTLATGMKTSIKVYFRKKVTEQYPENRKELKMFDRFRGTLAMPHNENNEHRCVACGLCQIAC
PNDTITVTSETIETEDGKKKKILAKYEYDLGACMFCQLCVNACPHDAITFDQNFEHAVFDRTKLVLQLNHAGSKVIEKKK
EV
>Mature_162_residues
MEYKDQKYTYLGGLIHGISTLATGMKTSIKVYFRKKVTEQYPENRKELKMFDRFRGTLAMPHNENNEHRCVACGLCQIAC
PNDTITVTSETIETEDGKKKKILAKYEYDLGACMFCQLCVNACPHDAITFDQNFEHAVFDRTKLVLQLNHAGSKVIEKKK
EV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1788617, Length=115, Percent_Identity=34.7826086956522, Blast_Score=77, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17555194, Length=150, Percent_Identity=32.6666666666667, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17864306, Length=138, Percent_Identity=32.6086956521739, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18564; Mature: 18564

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKDQKYTYLGGLIHGISTLATGMKTSIKVYFRKKVTEQYPENRKELKMFDRFRGTLAM
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEC
PHNENNEHRCVACGLCQIACPNDTITVTSETIETEDGKKKKILAKYEYDLGACMFCQLCV
CCCCCCCCEEEEECEEEEECCCCEEEEEECEEECCCCCCCEEEEEECCCCHHHHHHHHHH
NACPHDAITFDQNFEHAVFDRTKLVLQLNHAGSKVIEKKKEV
HCCCCCCEECCCCCCHHHHHHEEEEEEECCCCHHHHHHHCCC
>Mature Secondary Structure
MEYKDQKYTYLGGLIHGISTLATGMKTSIKVYFRKKVTEQYPENRKELKMFDRFRGTLAM
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEC
PHNENNEHRCVACGLCQIACPNDTITVTSETIETEDGKKKKILAKYEYDLGACMFCQLCV
CCCCCCCCEEEEECEEEEECCCCEEEEEECEEECCCCCCCEEEEEECCCCHHHHHHHHHH
NACPHDAITFDQNFEHAVFDRTKLVLQLNHAGSKVIEKKKEV
HCCCCCCEECCCCCCHHHHHHEEEEEEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA