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The map label for this gene is ndhF [H]

Identifier: 29349468

GI number: 29349468

Start: 5294040

End: 5295971

Strand: Reverse

Name: ndhF [H]

Synonym: BT_4060

Alternate gene names: 29349468

Gene position: 5295971-5294040 (Counterclockwise)

Preceding gene: 29349469

Following gene: 29349467

Centisome position: 84.6

GC content: 48.71

Gene sequence:

>1932_bases
ATGGAACTAACAATTCTAATACTCCTTCTTCCCTTCCTCTCCTTCCTCATTCTGGGAATCGGAGGAAAATGGATGTCTCA
TCGGACAGCGGGTGCTATCGGTACGCTGATACTGGGAGCAGTAGTAGTACTGTCGTACGTTACGGCTTTCCAATATTTCT
CCGCCCCTCGTTTGGAAGACGGAACATTTGCCACGTTGATACCTTATAATTTTACGTGGCTTCCTTTCACCGAGACTTTA
CGATTTGATCTGGGCATTCTGCTCGATCCCATATCCGTGATGATGCTGATTGTCATCTCTACCGTGTCACTGATGGTGCA
TATCTACTCCTTCGGTTACATGAAAGGCGAGACAGGCTTTCAACGTTATTACGCCTTCCTGTCCCTGTTTACAATGTCTA
TGCTGGGACTGGTGGTTGCCACAAACATTTTCCAGATGTATCTTTTCTGGGAACTGGTGGGTGTCAGTTCTTATCTGCTG
ATCGGTTTTTACTATACAAAGCCTGCTGCCATTGCCGCTTCCAAGAAAGCGTTCATTGTCACCCGCTTTGCAGACTTAGG
CTTCCTTATCGGTATTCTGATTTACGGATACTACGGTGGCACATTCGGCTTCACACCGGATACGGTATCATTGATCAGTG
GCGGCGCTTCCATGCTTCCATTGGCTTTGGGACTGATGTTTATCGGTGGCGCCGGAAAGAGTGCCATGTTCCCGTTGCAT
ATCTGGCTGCCCGATGCCATGGAAGGTCCTACTCCTGTCAGTGCCTTGATCCATGCGGCTACGATGGTAGTTGCCGGCGT
TTATCTGGTAGCGCGTATGTTCCCGCTTTTCATTGCTTATGCACCGGACACCTTACATATGATTGCCTGGGTAGGTGCTT
TCACCGCCTTTTATGCAGCAAGCGTGGCTTGCGTACAATCGGATATCAAACGTGTGCTGGCCTTCTCGACCATTTCACAG
ATTGGTTTTATGATGGTAGCACTGGGCGTTTGTACTTCTATGGACCCGCACCACGGAGGACTGGGATATATGGCTTCCAT
GTTCCACCTCTTCACACACGCCATGTTTAAGGCATTACTCTTCTTGGGTGCCGGCAGCATTATCCATGCAGTACATTCCA
ACGAAATGTCAGCCATGGGAGGACTACGCAAATACATGCCTATTACTCACTGGACATTCCTGATTGCCTGTCTCGCCATT
GCCGGTATTCCTCCGTTCTCCGGTTTCTTCTCAAAAGATGAGATTTTGGCTGCCTGCTTCCAATATAGCCCTGCAATGGG
TTGGGTGATGACAGTAATCGCCGCAATGACCGCCTTCTATATGTTCCGCCTCTACTACGGCATCTTCTGGGGGGCCGAGC
CGCCCCGGGCAAGCTCCCATTCGGATCATAGCACACCGCACGGAAACTTGGAAGCGGCACCTTGTCGCCCGCATGAGAGT
CCGTTGGCTATGACTTTTCCGTTGATATTTCTGGCGGTTGTTACCTGCCTTGCAGGATTCATTCCTTTCGGACATTTTAT
CAGTTCGAATGGGGAGTCTTACTCTATTCACCTCGATCTGTCGGTAGCTGTCACAAGCGTTGTAATTGCGATTATTTCTA
TCGGGATTGCCACATGGATGTATAAGAATGCCAAACAGCCTGTTGCCAATGCACTTGCCAAACGGTTCAATGGATTGTGG
ACAGCTGCTTATCATCGTTTTTATATTGATGATATATACCAGTTTATTACGCATAAGATCATCTTCCGTTGCATTTCGAC
TCCTATCGCATGGTTCGACCGTCATGTAGTGGATGGATTCTTTAATTTCCTGGCATGGGCTACGAATACGACAAGCGACG
AGATACGTGGTCTTCAAAGCGGCCAGGTACAGCAATACGCATACGTGTTCCTTTGCGGAGCGCTGGCGCTTATCCTGCTG
TTAGTTCTATAG

Upstream 100 bases:

>100_bases
TCTGAAGTGGTAGTGCCAAAGCGGCACGACCCAATTTCCATTCGGATGGATATCGTGATAAATATGAACAATAATAAAAC
AAGTAATTAAAATAAACTGT

Downstream 100 bases:

>100_bases
CAAGTATTAACTATCAGGTATTAGATATTAAGCTGCGCAACAAGTCGCTGCGAATTAATACTTAAAACTTAATAATTCAA
TTATGAATTTTTTATCAATC

Product: NADH dehydrogenase I subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MELTILILLLPFLSFLILGIGGKWMSHRTAGAIGTLILGAVVVLSYVTAFQYFSAPRLEDGTFATLIPYNFTWLPFTETL
RFDLGILLDPISVMMLIVISTVSLMVHIYSFGYMKGETGFQRYYAFLSLFTMSMLGLVVATNIFQMYLFWELVGVSSYLL
IGFYYTKPAAIAASKKAFIVTRFADLGFLIGILIYGYYGGTFGFTPDTVSLISGGASMLPLALGLMFIGGAGKSAMFPLH
IWLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIAYAPDTLHMIAWVGAFTAFYAASVACVQSDIKRVLAFSTISQ
IGFMMVALGVCTSMDPHHGGLGYMASMFHLFTHAMFKALLFLGAGSIIHAVHSNEMSAMGGLRKYMPITHWTFLIACLAI
AGIPPFSGFFSKDEILAACFQYSPAMGWVMTVIAAMTAFYMFRLYYGIFWGAEPPRASSHSDHSTPHGNLEAAPCRPHES
PLAMTFPLIFLAVVTCLAGFIPFGHFISSNGESYSIHLDLSVAVTSVVIAIISIGIATWMYKNAKQPVANALAKRFNGLW
TAAYHRFYIDDIYQFITHKIIFRCISTPIAWFDRHVVDGFFNFLAWATNTTSDEIRGLQSGQVQQYAYVFLCGALALILL
LVL

Sequences:

>Translated_643_residues
MELTILILLLPFLSFLILGIGGKWMSHRTAGAIGTLILGAVVVLSYVTAFQYFSAPRLEDGTFATLIPYNFTWLPFTETL
RFDLGILLDPISVMMLIVISTVSLMVHIYSFGYMKGETGFQRYYAFLSLFTMSMLGLVVATNIFQMYLFWELVGVSSYLL
IGFYYTKPAAIAASKKAFIVTRFADLGFLIGILIYGYYGGTFGFTPDTVSLISGGASMLPLALGLMFIGGAGKSAMFPLH
IWLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIAYAPDTLHMIAWVGAFTAFYAASVACVQSDIKRVLAFSTISQ
IGFMMVALGVCTSMDPHHGGLGYMASMFHLFTHAMFKALLFLGAGSIIHAVHSNEMSAMGGLRKYMPITHWTFLIACLAI
AGIPPFSGFFSKDEILAACFQYSPAMGWVMTVIAAMTAFYMFRLYYGIFWGAEPPRASSHSDHSTPHGNLEAAPCRPHES
PLAMTFPLIFLAVVTCLAGFIPFGHFISSNGESYSIHLDLSVAVTSVVIAIISIGIATWMYKNAKQPVANALAKRFNGLW
TAAYHRFYIDDIYQFITHKIIFRCISTPIAWFDRHVVDGFFNFLAWATNTTSDEIRGLQSGQVQQYAYVFLCGALALILL
LVL
>Mature_643_residues
MELTILILLLPFLSFLILGIGGKWMSHRTAGAIGTLILGAVVVLSYVTAFQYFSAPRLEDGTFATLIPYNFTWLPFTETL
RFDLGILLDPISVMMLIVISTVSLMVHIYSFGYMKGETGFQRYYAFLSLFTMSMLGLVVATNIFQMYLFWELVGVSSYLL
IGFYYTKPAAIAASKKAFIVTRFADLGFLIGILIYGYYGGTFGFTPDTVSLISGGASMLPLALGLMFIGGAGKSAMFPLH
IWLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIAYAPDTLHMIAWVGAFTAFYAASVACVQSDIKRVLAFSTISQ
IGFMMVALGVCTSMDPHHGGLGYMASMFHLFTHAMFKALLFLGAGSIIHAVHSNEMSAMGGLRKYMPITHWTFLIACLAI
AGIPPFSGFFSKDEILAACFQYSPAMGWVMTVIAAMTAFYMFRLYYGIFWGAEPPRASSHSDHSTPHGNLEAAPCRPHES
PLAMTFPLIFLAVVTCLAGFIPFGHFISSNGESYSIHLDLSVAVTSVVIAIISIGIATWMYKNAKQPVANALAKRFNGLW
TAAYHRFYIDDIYQFITHKIIFRCISTPIAWFDRHVVDGFFNFLAWATNTTSDEIRGLQSGQVQQYAYVFLCGALALILL
LVL

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=371, Percent_Identity=38.8140161725067, Blast_Score=234, Evalue=2e-61,
Organism=Escherichia coli, GI1788614, Length=591, Percent_Identity=38.409475465313, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI1788829, Length=519, Percent_Identity=31.5992292870906, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788827, Length=409, Percent_Identity=33.2518337408313, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788831, Length=342, Percent_Identity=30.4093567251462, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788613, Length=386, Percent_Identity=29.5336787564767, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI2367154, Length=516, Percent_Identity=24.031007751938, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=337, Percent_Identity=25.5192878338279, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 70661; Mature: 70661

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTILILLLPFLSFLILGIGGKWMSHRTAGAIGTLILGAVVVLSYVTAFQYFSAPRLED
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTFATLIPYNFTWLPFTETLRFDLGILLDPISVMMLIVISTVSLMVHIYSFGYMKGETGF
CCEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QRYYAFLSLFTMSMLGLVVATNIFQMYLFWELVGVSSYLLIGFYYTKPAAIAASKKAFIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEE
TRFADLGFLIGILIYGYYGGTFGFTPDTVSLISGGASMLPLALGLMFIGGAGKSAMFPLH
EHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IWLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIAYAPDTLHMIAWVGAFTAFYAA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SVACVQSDIKRVLAFSTISQIGFMMVALGVCTSMDPHHGGLGYMASMFHLFTHAMFKALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FLGAGSIIHAVHSNEMSAMGGLRKYMPITHWTFLIACLAIAGIPPFSGFFSKDEILAACF
HHCCCHHHHHHHCCCHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QYSPAMGWVMTVIAAMTAFYMFRLYYGIFWGAEPPRASSHSDHSTPHGNLEAAPCRPHES
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLAMTFPLIFLAVVTCLAGFIPFGHFISSNGESYSIHLDLSVAVTSVVIAIISIGIATWM
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
YKNAKQPVANALAKRFNGLWTAAYHRFYIDDIYQFITHKIIFRCISTPIAWFDRHVVDGF
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FNFLAWATNTTSDEIRGLQSGQVQQYAYVFLCGALALILLLVL
HHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MELTILILLLPFLSFLILGIGGKWMSHRTAGAIGTLILGAVVVLSYVTAFQYFSAPRLED
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTFATLIPYNFTWLPFTETLRFDLGILLDPISVMMLIVISTVSLMVHIYSFGYMKGETGF
CCEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QRYYAFLSLFTMSMLGLVVATNIFQMYLFWELVGVSSYLLIGFYYTKPAAIAASKKAFIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEE
TRFADLGFLIGILIYGYYGGTFGFTPDTVSLISGGASMLPLALGLMFIGGAGKSAMFPLH
EHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IWLPDAMEGPTPVSALIHAATMVVAGVYLVARMFPLFIAYAPDTLHMIAWVGAFTAFYAA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SVACVQSDIKRVLAFSTISQIGFMMVALGVCTSMDPHHGGLGYMASMFHLFTHAMFKALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FLGAGSIIHAVHSNEMSAMGGLRKYMPITHWTFLIACLAIAGIPPFSGFFSKDEILAACF
HHCCCHHHHHHHCCCHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QYSPAMGWVMTVIAAMTAFYMFRLYYGIFWGAEPPRASSHSDHSTPHGNLEAAPCRPHES
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLAMTFPLIFLAVVTCLAGFIPFGHFISSNGESYSIHLDLSVAVTSVVIAIISIGIATWM
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
YKNAKQPVANALAKRFNGLWTAAYHRFYIDDIYQFITHKIIFRCISTPIAWFDRHVVDGF
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FNFLAWATNTTSDEIRGLQSGQVQQYAYVFLCGALALILLLVL
HHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]