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The map label for this gene is mltD [H]

Identifier: 29349407

GI number: 29349407

Start: 5211775

End: 5213070

Strand: Reverse

Name: mltD [H]

Synonym: BT_3999

Alternate gene names: 29349407

Gene position: 5213070-5211775 (Counterclockwise)

Preceding gene: 29349408

Following gene: 29349406

Centisome position: 83.27

GC content: 45.06

Gene sequence:

>1296_bases
ATGAAGAAATTAGAAAACTATTGTTCGATTATTTTCCTTTTCCTGTTGGCAACCACACAGGTAAAAGCTCAAAGTGTAGA
TGTAGTTATTCGTGAAAACGGAACCGAACGCAAAGAAAGCATCGATCTTCCAAAGAGTATGACTTACCCGCTCGACAGTC
TGCTGAACGACTGGAAAGCTAAAAACTATATTGATTTAGGCAAAGATTGCAGTACGGCGGAAGTGAATCCTTTATTCAGT
GATTCTGTCTACATTGACCGCCTTTCACGTATTCCGGCTGTCATGGAAATGCCTTATAATGACATTGTACGCAAATTCAT
CGATATGTACGCGGGTCGCCTGCGCAATCAGGTTTCATTCATGCTGAGCGCCTGCAATTTCTATATGCCTATTTTCGAAG
AAGCACTGGACGCTTACGGACTTCCTTTGGAACTGAAGTACCTGCCGATTATCGAATCTGCACTGAATCCTTCAGCTGTG
TCACGTGCCGGTGCTTCTGGACTGTGGCAATTCATGATTGGAACAGGAAAGATTTACGGTCTTGAATCCAATAGTCTGAT
AGACGAACGCCGCGACCCGATCAAAGCAACCTGGGCTGCCGCCCGTTACTTGAAAGAGATGTACGATATTTATGGAGACT
GGAATCTGGTTATAGCAGCCTATAACTGTGGCCCAGGTACTATCAACAAGGCAATCCGCCGCGCCGGAGGCGAAACGGAT
TACTGGAAAATCTATAACCTGCTGCCTAAAGAGACACGCGGATATGTACCTGCTTTCATCGCAGCCAACTACGTGATGAC
ATACTATTGCGACCACAACATCTGTCCGATGGAAACAAACATTCCGGCAAGCACAGATACGGTGCAAGTCACTAAAAACC
TTCATTTCGAGCAGATTGCCGAACTTTGCAATGTTCCGCTGGAGGAAGTGAAAAGCCTGAATCCCCAATACAAGAAACAA
GTGATTCCCGGCACGACCAAACCTTGTACATTACGTTTACCACAAGGTGCCATCAGTACGTTTATTGACAAGCAAGATAC
AATCTACGCACACCGTGCCGACGAGCTATTCCGCAACCGCAAAACCGTTGCAGTGAAAGAAGTTACTCCAACCACCCGCA
GACAGACAAGCGCTGTTGCCGGTAAAGGAAAATTAACTTATTATAAGATAAAAAGTGGAGACACTCTTTCATCGATTGCC
GAAAAGCTAGGCGTACGTGTCAAAGATATACAGCAGTGGAACGGTATGTCGAACACCAGAATCGCAGCAGGAAAACAATT
GAAAATCTACAAATAA

Upstream 100 bases:

>100_bases
CAGCCAGTTCCGCTCCAACAGTTACAAGAATAAGTCAGTGGGACTGCAATGCGTACTACGATTTTAAAAACACAATTTAG
ATTGAACTTATAATTAAAGC

Downstream 100 bases:

>100_bases
ATGACTTCCCGCTTGCTTCATTCGGAAATTTGCTTATTTTTGCAAAAATAAGCGAATGAAAGGATAACGAATATGGATAA
TATAACCCCCAAAGAAATAG

Product: membrane-bound lytic murein transglycosylase D presursor

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKKLENYCSIIFLFLLATTQVKAQSVDVVIRENGTERKESIDLPKSMTYPLDSLLNDWKAKNYIDLGKDCSTAEVNPLFS
DSVYIDRLSRIPAVMEMPYNDIVRKFIDMYAGRLRNQVSFMLSACNFYMPIFEEALDAYGLPLELKYLPIIESALNPSAV
SRAGASGLWQFMIGTGKIYGLESNSLIDERRDPIKATWAAARYLKEMYDIYGDWNLVIAAYNCGPGTINKAIRRAGGETD
YWKIYNLLPKETRGYVPAFIAANYVMTYYCDHNICPMETNIPASTDTVQVTKNLHFEQIAELCNVPLEEVKSLNPQYKKQ
VIPGTTKPCTLRLPQGAISTFIDKQDTIYAHRADELFRNRKTVAVKEVTPTTRRQTSAVAGKGKLTYYKIKSGDTLSSIA
EKLGVRVKDIQQWNGMSNTRIAAGKQLKIYK

Sequences:

>Translated_431_residues
MKKLENYCSIIFLFLLATTQVKAQSVDVVIRENGTERKESIDLPKSMTYPLDSLLNDWKAKNYIDLGKDCSTAEVNPLFS
DSVYIDRLSRIPAVMEMPYNDIVRKFIDMYAGRLRNQVSFMLSACNFYMPIFEEALDAYGLPLELKYLPIIESALNPSAV
SRAGASGLWQFMIGTGKIYGLESNSLIDERRDPIKATWAAARYLKEMYDIYGDWNLVIAAYNCGPGTINKAIRRAGGETD
YWKIYNLLPKETRGYVPAFIAANYVMTYYCDHNICPMETNIPASTDTVQVTKNLHFEQIAELCNVPLEEVKSLNPQYKKQ
VIPGTTKPCTLRLPQGAISTFIDKQDTIYAHRADELFRNRKTVAVKEVTPTTRRQTSAVAGKGKLTYYKIKSGDTLSSIA
EKLGVRVKDIQQWNGMSNTRIAAGKQLKIYK
>Mature_431_residues
MKKLENYCSIIFLFLLATTQVKAQSVDVVIRENGTERKESIDLPKSMTYPLDSLLNDWKAKNYIDLGKDCSTAEVNPLFS
DSVYIDRLSRIPAVMEMPYNDIVRKFIDMYAGRLRNQVSFMLSACNFYMPIFEEALDAYGLPLELKYLPIIESALNPSAV
SRAGASGLWQFMIGTGKIYGLESNSLIDERRDPIKATWAAARYLKEMYDIYGDWNLVIAAYNCGPGTINKAIRRAGGETD
YWKIYNLLPKETRGYVPAFIAANYVMTYYCDHNICPMETNIPASTDTVQVTKNLHFEQIAELCNVPLEEVKSLNPQYKKQ
VIPGTTKPCTLRLPQGAISTFIDKQDTIYAHRADELFRNRKTVAVKEVTPTTRRQTSAVAGKGKLTYYKIKSGDTLSSIA
EKLGVRVKDIQQWNGMSNTRIAAGKQLKIYK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=344, Percent_Identity=31.1046511627907, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48754; Mature: 48754

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLENYCSIIFLFLLATTQVKAQSVDVVIRENGTERKESIDLPKSMTYPLDSLLNDWKA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCC
KNYIDLGKDCSTAEVNPLFSDSVYIDRLSRIPAVMEMPYNDIVRKFIDMYAGRLRNQVSF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MLSACNFYMPIFEEALDAYGLPLELKYLPIIESALNPSAVSRAGASGLWQFMIGTGKIYG
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHCCHHHHHHHEECCCEEEE
LESNSLIDERRDPIKATWAAARYLKEMYDIYGDWNLVIAAYNCGPGTINKAIRRAGGETD
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCC
YWKIYNLLPKETRGYVPAFIAANYVMTYYCDHNICPMETNIPASTDTVQVTKNLHFEQIA
HHHHHHCCCHHHCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEHHHCCCHHHHH
ELCNVPLEEVKSLNPQYKKQVIPGTTKPCTLRLPQGAISTFIDKQDTIYAHRADELFRNR
HHHCCCHHHHHCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCEEHHHHHHHHHCC
KTVAVKEVTPTTRRQTSAVAGKGKLTYYKIKSGDTLSSIAEKLGVRVKDIQQWNGMSNTR
CCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCE
IAAGKQLKIYK
EECCCEEEEEC
>Mature Secondary Structure
MKKLENYCSIIFLFLLATTQVKAQSVDVVIRENGTERKESIDLPKSMTYPLDSLLNDWKA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCC
KNYIDLGKDCSTAEVNPLFSDSVYIDRLSRIPAVMEMPYNDIVRKFIDMYAGRLRNQVSF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MLSACNFYMPIFEEALDAYGLPLELKYLPIIESALNPSAVSRAGASGLWQFMIGTGKIYG
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHCCHHHHHHHEECCCEEEE
LESNSLIDERRDPIKATWAAARYLKEMYDIYGDWNLVIAAYNCGPGTINKAIRRAGGETD
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCC
YWKIYNLLPKETRGYVPAFIAANYVMTYYCDHNICPMETNIPASTDTVQVTKNLHFEQIA
HHHHHHCCCHHHCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEHHHCCCHHHHH
ELCNVPLEEVKSLNPQYKKQVIPGTTKPCTLRLPQGAISTFIDKQDTIYAHRADELFRNR
HHHCCCHHHHHCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCEEHHHHHHHHHCC
KTVAVKEVTPTTRRQTSAVAGKGKLTYYKIKSGDTLSSIAEKLGVRVKDIQQWNGMSNTR
CCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCE
IAAGKQLKIYK
EECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]