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The map label for this gene is queA [H]

Identifier: 29349381

GI number: 29349381

Start: 5173216

End: 5174436

Strand: Reverse

Name: queA [H]

Synonym: BT_3973

Alternate gene names: 29349381

Gene position: 5174436-5173216 (Counterclockwise)

Preceding gene: 29349383

Following gene: 29349380

Centisome position: 82.65

GC content: 49.3

Gene sequence:

>1221_bases
ATGAAAGAAGACCCGAAACATATTCACATCAGCGAATACAACTATCCACTACCGGATGAACGAATCGCCAAATTTCCTTT
AGCAGTACGAGACCAGTCAAAACTGTTGGTATATCGCCACGGTGAAGTAAGCGAAGATGTCTTCACATCCTTGCCGGACT
ATCTCCCGAAAGGAAGCCTGATGGTATTCAACAACACGAAGGTGATTCAGGCACGCCTCCACTTCCGCAAAGAAACGGGG
GCGCTGATCGAGGTCTTCTGTCTGGAACCGATTCAGCCCAACGATTATGTATTGAACTTCCAGCAGACGGAACACGCTGC
CTGGCTCTGCATGATCGGTAACCTGAAGAAATGGAAAGACGGAGCACTGAAACGGGAGATGACGGTAAAAGGTTTCCCGA
TCACTCTGACCGCTACCCGTGGAGAATGCAGGGGCACCAGCCACTGGATTGATTTCTCCTGGGACAATCCGGAAGTAACC
TTTGCCGATATCCTCGAAGTGTTCGGAGAACTGCCGATTCCCCCATATCTGAACCGGGATACGGAAGAGAGCGATAAAGA
AACTTATCAGACCGTTTATTCCAAAATAAAAGGCTCGGTAGCCGCACCGACAGCCGGACTGCACTTCACTCCCCGTGTAC
TGGAAGCATTGCAGGAAAAAGGCATCGATCTGGAAGAACTGACACTTCATGTAGGCGCGGGAACCTTCAAGCCCGTGAAA
AGCGAAGAGATAGAAGGGCATGAGATGCATACGGAGTATATCTCCGTCAATCGGGCTACGATCAAAAAGCTGATCGATCA
TGACGGTTGCGCTGTTGCTGTAGGCACCACTTCGGTGCGTACGCTCGAAAGCCTGTATCACATCGGAGTAACATTGGCGG
AAAATCCCGATGCTACGGAAGAAGAACTGCACGTCAAGCAATGGCAGCCATACGAAAAGTATGATCAGATTCCGCCTGTA
GTCGCACTGCAAAAGATTTTAGGTTACCTCGACAGGAACGGGCTGGAAGCCCTTCACAGCAGTACGCAGATTATCATAGC
TCCGGGATATCAGTATAAGATAGTGAAAGCGATGGTGACCAACTTTCACCAGCCGCAAAGTACACTGTTACTTTTGGTTT
CCGCCTTCGTGAAGGGAAACTGGCGCACAATTTATGATTATGCGCTGGCGCACGATTTCCGTTTTCTGAGTTATGGAGAT
TCTTCTCTGCTGATACCGTAA

Upstream 100 bases:

>100_bases
CTGGGGGGCGTGTGGTCGCAAGTTCGAATCTTGTCATCCCGACTACCGACAAAGCATTGATAATCGATGGGTTATTGATG
CTTTTTTTATTGAAACGAGA

Downstream 100 bases:

>100_bases
ACCAAAAAGTAAAAACAACTATGCCAAGCGATAATAATCAAGAAATGTTTCCCATCGTAGACGAACAGGGAAACATCACC
GGAGCCGCCACACGCGGAGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKEDPKHIHISEYNYPLPDERIAKFPLAVRDQSKLLVYRHGEVSEDVFTSLPDYLPKGSLMVFNNTKVIQARLHFRKETG
ALIEVFCLEPIQPNDYVLNFQQTEHAAWLCMIGNLKKWKDGALKREMTVKGFPITLTATRGECRGTSHWIDFSWDNPEVT
FADILEVFGELPIPPYLNRDTEESDKETYQTVYSKIKGSVAAPTAGLHFTPRVLEALQEKGIDLEELTLHVGAGTFKPVK
SEEIEGHEMHTEYISVNRATIKKLIDHDGCAVAVGTTSVRTLESLYHIGVTLAENPDATEEELHVKQWQPYEKYDQIPPV
VALQKILGYLDRNGLEALHSSTQIIIAPGYQYKIVKAMVTNFHQPQSTLLLLVSAFVKGNWRTIYDYALAHDFRFLSYGD
SSLLIP

Sequences:

>Translated_406_residues
MKEDPKHIHISEYNYPLPDERIAKFPLAVRDQSKLLVYRHGEVSEDVFTSLPDYLPKGSLMVFNNTKVIQARLHFRKETG
ALIEVFCLEPIQPNDYVLNFQQTEHAAWLCMIGNLKKWKDGALKREMTVKGFPITLTATRGECRGTSHWIDFSWDNPEVT
FADILEVFGELPIPPYLNRDTEESDKETYQTVYSKIKGSVAAPTAGLHFTPRVLEALQEKGIDLEELTLHVGAGTFKPVK
SEEIEGHEMHTEYISVNRATIKKLIDHDGCAVAVGTTSVRTLESLYHIGVTLAENPDATEEELHVKQWQPYEKYDQIPPV
VALQKILGYLDRNGLEALHSSTQIIIAPGYQYKIVKAMVTNFHQPQSTLLLLVSAFVKGNWRTIYDYALAHDFRFLSYGD
SSLLIP
>Mature_406_residues
MKEDPKHIHISEYNYPLPDERIAKFPLAVRDQSKLLVYRHGEVSEDVFTSLPDYLPKGSLMVFNNTKVIQARLHFRKETG
ALIEVFCLEPIQPNDYVLNFQQTEHAAWLCMIGNLKKWKDGALKREMTVKGFPITLTATRGECRGTSHWIDFSWDNPEVT
FADILEVFGELPIPPYLNRDTEESDKETYQTVYSKIKGSVAAPTAGLHFTPRVLEALQEKGIDLEELTLHVGAGTFKPVK
SEEIEGHEMHTEYISVNRATIKKLIDHDGCAVAVGTTSVRTLESLYHIGVTLAENPDATEEELHVKQWQPYEKYDQIPPV
VALQKILGYLDRNGLEALHSSTQIIIAPGYQYKIVKAMVTNFHQPQSTLLLLVSAFVKGNWRTIYDYALAHDFRFLSYGD
SSLLIP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=404, Percent_Identity=37.1287128712871, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 46104; Mature: 46104

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDPKHIHISEYNYPLPDERIAKFPLAVRDQSKLLVYRHGEVSEDVFTSLPDYLPKGSL
CCCCCCEEEEECCCCCCCHHHHHHCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCE
MVFNNTKVIQARLHFRKETGALIEVFCLEPIQPNDYVLNFQQTEHAAWLCMIGNLKKWKD
EEECCCEEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHCC
GALKREMTVKGFPITLTATRGECRGTSHWIDFSWDNPEVTFADILEVFGELPIPPYLNRD
CCCCCEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCC
TEESDKETYQTVYSKIKGSVAAPTAGLHFTPRVLEALQEKGIDLEELTLHVGAGTFKPVK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCC
SEEIEGHEMHTEYISVNRATIKKLIDHDGCAVAVGTTSVRTLESLYHIGVTLAENPDATE
CCCCCCCCEEHHHEECCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCEEEECCCCCCH
EELHVKQWQPYEKYDQIPPVVALQKILGYLDRNGLEALHSSTQIIIAPGYQYKIVKAMVT
HHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCHHHHHHHHH
NFHQPQSTLLLLVSAFVKGNWRTIYDYALAHDFRFLSYGDSSLLIP
CCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCEEEEEECCCEEECC
>Mature Secondary Structure
MKEDPKHIHISEYNYPLPDERIAKFPLAVRDQSKLLVYRHGEVSEDVFTSLPDYLPKGSL
CCCCCCEEEEECCCCCCCHHHHHHCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCE
MVFNNTKVIQARLHFRKETGALIEVFCLEPIQPNDYVLNFQQTEHAAWLCMIGNLKKWKD
EEECCCEEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHCC
GALKREMTVKGFPITLTATRGECRGTSHWIDFSWDNPEVTFADILEVFGELPIPPYLNRD
CCCCCEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCC
TEESDKETYQTVYSKIKGSVAAPTAGLHFTPRVLEALQEKGIDLEELTLHVGAGTFKPVK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCC
SEEIEGHEMHTEYISVNRATIKKLIDHDGCAVAVGTTSVRTLESLYHIGVTLAENPDATE
CCCCCCCCEEHHHEECCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCEEEECCCCCCH
EELHVKQWQPYEKYDQIPPVVALQKILGYLDRNGLEALHSSTQIIIAPGYQYKIVKAMVT
HHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCHHHHHHHHH
NFHQPQSTLLLLVSAFVKGNWRTIYDYALAHDFRFLSYGDSSLLIP
CCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA