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The map label for this gene is tolC [C]

Identifier: 29346660

GI number: 29346660

Start: 1556809

End: 1558128

Strand: Reverse

Name: tolC [C]

Synonym: BT_1250

Alternate gene names: 29346660

Gene position: 1558128-1556809 (Counterclockwise)

Preceding gene: 29346661

Following gene: 29346659

Centisome position: 24.89

GC content: 48.03

Gene sequence:

>1320_bases
ATGTTCTTGAAAACGTATAGGGTATATCTGATGGGGCCGCTCTGCCTGATGTTCGCCGGTCAGCCGGCAAAGGCGCAGAC
GGATTCTTTATTTCTTTCCGTAGACCAGCTTTTCGAACGGGGGGTGCAGCATAGTTTGCAGCTTCAGGCGGATGCGCTCA
AAGAAGCCATGGCGCAAGAACGGACACGGACGGCACGCACATCATCACTGCCCGATCTGCAAGTGGGATTGAAAGGAGGT
TTTGTGGGACAGCCGGTCGTTTGGGAACGGGGATTGTCCGGCCCGACATATCCGGATATTCCGGACTGGTCACAGAATTA
CGCCATCGACTTTTCCCAACCACTGTATCAAGGTGGCAAGATTCGGAGGACTATCCATAAGGCAGAGATGGAAAAACAAG
TTGCCGAGTTGCAGACATTAACAGACCAGGCGGAAATCAAACTTGGTTTGCTGAACCAATACATGAATCTGTTCAGCCTT
TTCAAGCAGCATGAGATATTGATGCGGAACATTGAGGAATCCGAATTACGCCTGCGGGATATTCGCCGGATGAAAAAGGA
GGGGGTGATAACGAATAATGATGTATTGCGCAGCGAGATGCAGTTGACGAACGACCGTTTGTCTTTGCAGGAAACGGAAA
ATAGCATCGTACTCGTGTCGCAGCAGCTGGATATTTTATTGGGACAGGATGAGAACCTGTTACTGAAACCGGATACGACC
CTACTGCATCAGGCAGTTGCTCTGGAAGCGTATGACGACTATATCACCCTTGCTTACACAAATGATCCGGCAATGAAATT
GCTCAGAAAACAAACGGAACTTGCCCGGAACGAGATCCGGTTGGCTCAGTCGTTGTCTCTTCCCAGCATATCGCTGTATG
CCTCAAATACACTGGCACGCCCTGTTTCGCGTACATTGACGGATATGTACAACAATAACTGGAATGTAGGACTGTCGGTT
TCTTATCCTTTATCCTCAATCTACAAAAACAGTCATAAAATAAAGGAAAGCAAACTGATGGTGTCTTTGCGTAAGAATGA
TGAAGAGCAGAAGATGCAGAGAATCCGTATGGATGTGCGCACTGCTTTTCTGCGGCATCAGGAAGCTTTGCAACGGGTAG
AAGCCTTACAGTTGTCTGTCCGGCAGGCGCAGGAGAACTATCGTATTATGCAGAACCGTTATCTGAATCAGTTGGCTATT
CTGACTGACTTGCTGGATGCCAACTCTGTCCGTTTGAATGTAGAGTTGCAGCTGGTCACTGCCCGCACTCGTGTGATTTA
TACTTATTATCAACTTCAGAAGGCTTGTGGTCGTCTATAA

Upstream 100 bases:

>100_bases
CAGATTCGTCCCATACTGGATCAGGTTTATGTATACGCAGAACAGGTGATTGGCATAGAAAGCATTGAACTCATGTTATC
GGAATTAAAGGGAGTATACG

Downstream 100 bases:

>100_bases
TATATTAGGGTTATGGCAACATTAAAGGAAAAAAAACGGAAACTTAAAAAAATGAGAGCTCGCAACATCATATTAAATAT
GGTGTGTGTATGTTTGGCTG

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: Outer Membrane Protein; Acriflavin Resistance Protein; Type I Secretion Outer Membrane Protein; RND Family Efflux Pump Inner Membrane Protein; LC Family Type I Secretion Outer Membrane Protein; Outer Membrane Protein CyaE; Outer Membrane Efflux Family Protein

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MFLKTYRVYLMGPLCLMFAGQPAKAQTDSLFLSVDQLFERGVQHSLQLQADALKEAMAQERTRTARTSSLPDLQVGLKGG
FVGQPVVWERGLSGPTYPDIPDWSQNYAIDFSQPLYQGGKIRRTIHKAEMEKQVAELQTLTDQAEIKLGLLNQYMNLFSL
FKQHEILMRNIEESELRLRDIRRMKKEGVITNNDVLRSEMQLTNDRLSLQETENSIVLVSQQLDILLGQDENLLLKPDTT
LLHQAVALEAYDDYITLAYTNDPAMKLLRKQTELARNEIRLAQSLSLPSISLYASNTLARPVSRTLTDMYNNNWNVGLSV
SYPLSSIYKNSHKIKESKLMVSLRKNDEEQKMQRIRMDVRTAFLRHQEALQRVEALQLSVRQAQENYRIMQNRYLNQLAI
LTDLLDANSVRLNVELQLVTARTRVIYTYYQLQKACGRL

Sequences:

>Translated_439_residues
MFLKTYRVYLMGPLCLMFAGQPAKAQTDSLFLSVDQLFERGVQHSLQLQADALKEAMAQERTRTARTSSLPDLQVGLKGG
FVGQPVVWERGLSGPTYPDIPDWSQNYAIDFSQPLYQGGKIRRTIHKAEMEKQVAELQTLTDQAEIKLGLLNQYMNLFSL
FKQHEILMRNIEESELRLRDIRRMKKEGVITNNDVLRSEMQLTNDRLSLQETENSIVLVSQQLDILLGQDENLLLKPDTT
LLHQAVALEAYDDYITLAYTNDPAMKLLRKQTELARNEIRLAQSLSLPSISLYASNTLARPVSRTLTDMYNNNWNVGLSV
SYPLSSIYKNSHKIKESKLMVSLRKNDEEQKMQRIRMDVRTAFLRHQEALQRVEALQLSVRQAQENYRIMQNRYLNQLAI
LTDLLDANSVRLNVELQLVTARTRVIYTYYQLQKACGRL
>Mature_439_residues
MFLKTYRVYLMGPLCLMFAGQPAKAQTDSLFLSVDQLFERGVQHSLQLQADALKEAMAQERTRTARTSSLPDLQVGLKGG
FVGQPVVWERGLSGPTYPDIPDWSQNYAIDFSQPLYQGGKIRRTIHKAEMEKQVAELQTLTDQAEIKLGLLNQYMNLFSL
FKQHEILMRNIEESELRLRDIRRMKKEGVITNNDVLRSEMQLTNDRLSLQETENSIVLVSQQLDILLGQDENLLLKPDTT
LLHQAVALEAYDDYITLAYTNDPAMKLLRKQTELARNEIRLAQSLSLPSISLYASNTLARPVSRTLTDMYNNNWNVGLSV
SYPLSSIYKNSHKIKESKLMVSLRKNDEEQKMQRIRMDVRTAFLRHQEALQRVEALQLSVRQAQENYRIMQNRYLNQLAI
LTDLLDANSVRLNVELQLVTARTRVIYTYYQLQKACGRL

Specific function: Required For Proper Expression Of Outer Membrane Protein Genes Such As Ompf, Nmpc, Protein 2, Hemolysin, Colicin V, Or Colicin E1. May Be Specialized For Signal Sequence Independent, Extracellular Secretion In Gram-Negative Bacteria. [C]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Outer Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50579; Mature: 50579

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKTYRVYLMGPLCLMFAGQPAKAQTDSLFLSVDQLFERGVQHSLQLQADALKEAMAQE
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTRTARTSSLPDLQVGLKGGFVGQPVVWERGLSGPTYPDIPDWSQNYAIDFSQPLYQGGK
HHHHHHCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCH
IRRTIHKAEMEKQVAELQTLTDQAEIKLGLLNQYMNLFSLFKQHEILMRNIEESELRLRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRRMKKEGVITNNDVLRSEMQLTNDRLSLQETENSIVLVSQQLDILLGQDENLLLKPDTT
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEEECEEEEECCCCCEEECCCHH
LLHQAVALEAYDDYITLAYTNDPAMKLLRKQTELARNEIRLAQSLSLPSISLYASNTLAR
HHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
PVSRTLTDMYNNNWNVGLSVSYPLSSIYKNSHKIKESKLMVSLRKNDEEQKMQRIRMDVR
HHHHHHHHHHCCCCCEEEEEECCHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
TAFLRHQEALQRVEALQLSVRQAQENYRIMQNRYLNQLAILTDLLDANSVRLNVELQLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
ARTRVIYTYYQLQKACGRL
ECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLKTYRVYLMGPLCLMFAGQPAKAQTDSLFLSVDQLFERGVQHSLQLQADALKEAMAQE
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTRTARTSSLPDLQVGLKGGFVGQPVVWERGLSGPTYPDIPDWSQNYAIDFSQPLYQGGK
HHHHHHCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCH
IRRTIHKAEMEKQVAELQTLTDQAEIKLGLLNQYMNLFSLFKQHEILMRNIEESELRLRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRRMKKEGVITNNDVLRSEMQLTNDRLSLQETENSIVLVSQQLDILLGQDENLLLKPDTT
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEEECEEEEECCCCCEEECCCHH
LLHQAVALEAYDDYITLAYTNDPAMKLLRKQTELARNEIRLAQSLSLPSISLYASNTLAR
HHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
PVSRTLTDMYNNNWNVGLSVSYPLSSIYKNSHKIKESKLMVSLRKNDEEQKMQRIRMDVR
HHHHHHHHHHCCCCCEEEEEECCHHHHHHCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
TAFLRHQEALQRVEALQLSVRQAQENYRIMQNRYLNQLAILTDLLDANSVRLNVELQLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
ARTRVIYTYYQLQKACGRL
ECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA