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The map label for this gene is nqrF [H]

Identifier: 29346565

GI number: 29346565

Start: 1441178

End: 1442446

Strand: Reverse

Name: nqrF [H]

Synonym: BT_1155

Alternate gene names: 29346565

Gene position: 1442446-1441178 (Counterclockwise)

Preceding gene: 29346566

Following gene: 29346564

Centisome position: 23.04

GC content: 46.57

Gene sequence:

>1269_bases
ATGAATTTAATATTATCAAGCATTGGGGTATTCCTTGTGGTTATTCTGTTGCTTGTTGTTATCTTGCTGGTTGCCAAGAA
ATTTCTGGTACCGTCAGGTAACGTGAAACTAACGATCAATGGCGAAACAGGACTGGAAGTAGAGTCCGGTTCCACATTGC
TTAATACGCTGGCCGTAAACGGTGTATTCCTGTCATCCGCTTGTGGTGGCAAGGGTTCTTGCGGACAGTGCAAATGTCAG
GTACTCGAAGGTGGTGGCGAAATCCTGCCTTCTGAAGTTCCTCACTTCAGCCGTAAACAGCAACAAGACCACTGGCGTCT
GGGCTGTCAGGTGAAAGTGAAGAGTGACATGGCTATCAAGATTGACGAGTCTGTACTCGGCGTGAAAGAGTGGGAGTGCG
AAGTGATCTCCAACAAGAATGTGGCTACATTTATCAAAGAGTTTATCGTGGCATTGCCTAAAGGCGAACACATGGACTTT
ATCCCGGGTTCTTACGCACAGATCAAGATACCTAAATTCTCAATGGATTATGATAAGGACATTGATAAGAGCCTGATCGG
TGAAGAATATCTGCCTGCATGGGAGAAATTCGGTTTGCTGGGCTTGAAATGTAAGAATGAAGAGGAAACAATCCGTGCTT
ACTCTATGGCCAACTATCCTGCAGAAGGTGACCGTATCATGCTGACTGTACGTATCGCTACCCCTCCGTTCAAGCCGAAA
GAACAAGGTCCCGGATTTATGGATGTAATGCCGGGTATCGCTTCTTCTTATATCTTTACGCTGAAACCGGGTGACAAGGT
AATAATGAGTGGGCCTTATGGAGACTTCCATCCGATTTTCGACTCTAACAAGGAAATGATGTGGATCGGTGGTGGTGCCG
GTATGGCTCCGTTGCGTGCACAGATCATGCACTTGACGAAAACATTGCATACGACCGACCGCAAGATGTCATACTTCTAT
GGTGCCCGTGCGCTGAACGAAGTATTCTATCTGGAAGACTTCCTGCAAATTGAAAAGGATTTCCCGAACTTCACATTCCA
TCTGGCACTCGACCGTCCGGATCCTGCTGCTGATGCTGCCGGTGTGAAATATACTCCGGGCTTCGTACACAACGTAATTT
ATGAAACTTACTTGAAGAACCACGAAGCTCCTGAAGATATTGAATACTATATGTGTGGTCCTGGTCCGATGTCGAAAGCT
GTAGAGAAGATGCTCGACGATCTGGGCGTTCCGGCTCAGAACCTGATGTTCGATAACTTTGGAGGATAA

Upstream 100 bases:

>100_bases
AGGTCTGGGTATCACATTTATCACTGTAGGTCTGATGGCAATCGCATTTATGTGTTTCTCTGGTTTGAACATCTAAAAAG
AAAGGAATAAGACAATGGAT

Downstream 100 bases:

>100_bases
TCTTCGGAAGTTGATTTCCGGTCGGAGAGGACCGGAAGAGTAACATAAAGAAACTGGCGGGAAATCTGTAAAAGACAATG
GTGCTTTTCAGATTTCCCGC

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVNGVFLSSACGGKGSCGQCKCQ
VLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIKIDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDF
IPGSYAQIKIPKFSMDYDKDIDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRAQIMHLTKTLHTTDRKMSYFY
GARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAAGVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKA
VEKMLDDLGVPAQNLMFDNFGG

Sequences:

>Translated_422_residues
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVNGVFLSSACGGKGSCGQCKCQ
VLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIKIDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDF
IPGSYAQIKIPKFSMDYDKDIDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRAQIMHLTKTLHTTDRKMSYFY
GARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAAGVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKA
VEKMLDDLGVPAQNLMFDNFGG
>Mature_422_residues
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVNGVFLSSACGGKGSCGQCKCQ
VLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIKIDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDF
IPGSYAQIKIPKFSMDYDKDIDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRAQIMHLTKTLHTTDRKMSYFY
GARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAAGVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKA
VEKMLDDLGVPAQNLMFDNFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 46729; Mature: 46729

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCHHHHHHHHC
GVFLSSACGGKGSCGQCKCQVLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIK
CEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCHHEECEEEEECCCEEEE
IDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPKFSMDYDKD
ECCHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
IDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
HHHHHCCCHHCCHHHHCCEEEEEECCCHHHEEHHEECCCCCCCCEEEEEEEEECCCCCCC
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRA
CCCCCHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHH
QIMHLTKTLHTTDRKMSYFYGARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
GVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKAVEKMLDDLGVPAQNLMFDNF
CCEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCC
GG
CC
>Mature Secondary Structure
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCHHHHHHHHC
GVFLSSACGGKGSCGQCKCQVLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIK
CEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCHHEECEEEEECCCEEEE
IDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPKFSMDYDKD
ECCHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
IDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
HHHHHCCCHHCCHHHHCCEEEEEECCCHHHEEHHEECCCCCCCCEEEEEEEEECCCCCCC
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRA
CCCCCHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHH
QIMHLTKTLHTTDRKMSYFYGARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
GVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKAVEKMLDDLGVPAQNLMFDNF
CCEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA