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The map label for this gene is sefB [H]

Identifier: 29144774

GI number: 29144774

Start: 4680464

End: 4681216

Strand: Direct

Name: sefB [H]

Synonym: t4534

Alternate gene names: 29144774

Gene position: 4680464-4681216 (Clockwise)

Preceding gene: 29144773

Following gene: 29144775

Centisome position: 97.67

GC content: 28.82

Gene sequence:

>753_bases
ATGTATATTTTGAATAAATGTATACGTATAACTGTTATCTTTTTCTTTTTTTGCTACCTTCCAATTGCTTCTTCGGAAAG
TAAAAAAATTGAGCAACCATTATTAACACAAAAATATTATGGCCTAAGATTGGGCACTACACGTGTTATTTATAAAGAAG
ATGCTCCATCAACAAGTTTTTGGATTATGAATGAAAAAGAATATCCAATCCTTGTTCAAACTCAAGTATATAATGATGAT
AAATCATCAAAAGCTCCATTTATTGTAACACCACCTATTTTGAAAGTTGAAAGTAATGCGCGAACAAGATTGAAGGTAAT
ACCAACAAGTAATCTATTCAATAAAAATGAGGAGTCTTTGTATTGGTTGTGTGTAAAAGGAGTCCCACCACTAAATGATA
ATGAAAGCAATAATAAAAACAACATAACTACGAATCTTAATGTGAATGTGGTTACGAATAGTTGTATTAAATTAATTTAT
AGGCCTAAAACTATAGACTTAACGACAATGGAGATTGCAGATAAATTAAAGTTAGAGAGAAAAGGAAATAGTATAGTTAT
AAAGAATCCAACATCATCATATGTGAATATTGCAAATATTAAATCTGGTAATTTAAGTTTTAATATTCCAAATGGATATA
TTGAGCCATTTGGATATGCTCAATTACCTGGTGGAGTACATAGTAAAATAACTTTGACTATTTTGGATGATAACGGCGCT
GAAATTATAAGAGATTATTATTTTAAGGTGTAA

Upstream 100 bases:

>100_bases
GCCCTCAATATGAGGGCATTTGGATAATTTTATTATTTTAAAAATATCTATTTTGAATAGATAGGTTTTATGCTTCCATG
CAAAAACTTAAAGAGGGATT

Downstream 100 bases:

>100_bases
ACAAATGAAGAAAACCACAATTACTCTATTTGTTTTAACCAGTGTATTTCACTCTGGAAATGTTTTCTCCAGACAATATA
ATTTCGACTATGGAAGTTTG

Product: fimbrial chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MYILNKCIRITVIFFFFCYLPIASSESKKIEQPLLTQKYYGLRLGTTRVIYKEDAPSTSFWIMNEKEYPILVQTQVYNDD
KSSKAPFIVTPPILKVESNARTRLKVIPTSNLFNKNEESLYWLCVKGVPPLNDNESNNKNNITTNLNVNVVTNSCIKLIY
RPKTIDLTTMEIADKLKLERKGNSIVIKNPTSSYVNIANIKSGNLSFNIPNGYIEPFGYAQLPGGVHSKITLTILDDNGA
EIIRDYYFKV

Sequences:

>Translated_250_residues
MYILNKCIRITVIFFFFCYLPIASSESKKIEQPLLTQKYYGLRLGTTRVIYKEDAPSTSFWIMNEKEYPILVQTQVYNDD
KSSKAPFIVTPPILKVESNARTRLKVIPTSNLFNKNEESLYWLCVKGVPPLNDNESNNKNNITTNLNVNVVTNSCIKLIY
RPKTIDLTTMEIADKLKLERKGNSIVIKNPTSSYVNIANIKSGNLSFNIPNGYIEPFGYAQLPGGVHSKITLTILDDNGA
EIIRDYYFKV
>Mature_250_residues
MYILNKCIRITVIFFFFCYLPIASSESKKIEQPLLTQKYYGLRLGTTRVIYKEDAPSTSFWIMNEKEYPILVQTQVYNDD
KSSKAPFIVTPPILKVESNARTRLKVIPTSNLFNKNEESLYWLCVKGVPPLNDNESNNKNNITTNLNVNVVTNSCIKLIY
RPKTIDLTTMEIADKLKLERKGNSIVIKNPTSSYVNIANIKSGNLSFNIPNGYIEPFGYAQLPGGVHSKITLTILDDNGA
EIIRDYYFKV

Specific function: Required for the biogenesis of the sefA (SEF14) fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786743, Length=235, Percent_Identity=29.7872340425532, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790771, Length=204, Percent_Identity=31.8627450980392, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789532, Length=184, Percent_Identity=31.5217391304348, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787171, Length=205, Percent_Identity=27.8048780487805, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788428, Length=208, Percent_Identity=28.8461538461538, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788677, Length=206, Percent_Identity=29.6116504854369, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786936, Length=205, Percent_Identity=25.3658536585366, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786333, Length=169, Percent_Identity=30.1775147928994, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 28481; Mature: 28481

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYILNKCIRITVIFFFFCYLPIASSESKKIEQPLLTQKYYGLRLGTTRVIYKEDAPSTSF
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCEEECCEEEEEECCCCCCEE
WIMNEKEYPILVQTQVYNDDKSSKAPFIVTPPILKVESNARTRLKVIPTSNLFNKNEESL
EEECCCCCCEEEEEEEECCCCCCCCCEEECCCEEEECCCCCEEEEEEECCCCCCCCCCEE
YWLCVKGVPPLNDNESNNKNNITTNLNVNVVTNSCIKLIYRPKTIDLTTMEIADKLKLER
EEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCEEEEEECCCEEEEEHHHHHHHHEEEE
KGNSIVIKNPTSSYVNIANIKSGNLSFNIPNGYIEPFGYAQLPGGVHSKITLTILDDNGA
CCCEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCEECCCCCCCEEEEEEECCCCH
EIIRDYYFKV
HHHHHHHCCC
>Mature Secondary Structure
MYILNKCIRITVIFFFFCYLPIASSESKKIEQPLLTQKYYGLRLGTTRVIYKEDAPSTSF
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCEEECCEEEEEECCCCCCEE
WIMNEKEYPILVQTQVYNDDKSSKAPFIVTPPILKVESNARTRLKVIPTSNLFNKNEESL
EEECCCCCCEEEEEEEECCCCCCCCCEEECCCEEEECCCCCEEEEEEECCCCCCCCCCEE
YWLCVKGVPPLNDNESNNKNNITTNLNVNVVTNSCIKLIYRPKTIDLTTMEIADKLKLER
EEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCEEEEEECCCEEEEEHHHHHHHHEEEE
KGNSIVIKNPTSSYVNIANIKSGNLSFNIPNGYIEPFGYAQLPGGVHSKITLTILDDNGA
CCCEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCEECCCCCCCEEEEEEECCCCH
EIIRDYYFKV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8097515 [H]