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The map label for this gene is intB [H]

Identifier: 29144761

GI number: 29144761

Start: 4666742

End: 4668007

Strand: Direct

Name: intB [H]

Synonym: t4518

Alternate gene names: 29144761

Gene position: 4666742-4668007 (Clockwise)

Preceding gene: 29144759

Following gene: 29144768

Centisome position: 97.39

GC content: 49.61

Gene sequence:

>1266_bases
ATGAAGCTCAACGCCAGACAGGTCGAGACCGCAAAGCCAAAAGACAAAACCTACAAAATGGCCGATGGCGGCGGTTTGTA
CCTTGAGGTTTCGGCCAAGGGATCCAAATACTGGCGCATGAAATACAGACGCCCCTCTGACAAAAAAGAGGATCGCCTCG
CTTTTGGTGTTTGGCCTACTGTGACACTTGCTCAGGCAAGAGCAAAGCGCGATGAAGCCAAAAAGTTGCTAGTGCAGGGC
ATTGATCCAAAAACCGAACAGAAAGAGGCTCAGGCCGAGAACTCGGGGGCGTATACTTTCGAAACTATCGCTCGTGAATG
GCATGCCAGTAACAAGCGCTGGAGCGAAGACCATCGATCGCGCGTTCTTCGCTATCTTGAACTTTATATTTTCCCTCATA
TCGGTTCGTCCGACATTCGCCAGCTCAAAACCAGTCACCTGTTAGCCCCGATCAAAAAAGTTGATGCCAGTGGTAAACAC
GACGTCGCTCAGCGCCTGCAACAGCGCGTCACGGCTATTATGCGTTATGCCGTACAGAACGATTACATCGACTCTAATCC
AGCCAGTGACATGGCCGGTGCGTTATCGACTACCAAAGCACGACACTATCCAGCCTTACCTTCTAGCCGTTTCCCTGAGT
TTCTTGCTCGCCTTGCTGCATATCGTGGCCGTGTAATGACACGGATTGCTGTCGAGCTTTCCTTACTAACTTTTGTACGT
TCCAGCGAGTTACGTTTCGCGCGTTGGGACGAGTTCGACTTCGATAAATCCCTTTGGCGCGTACCGGCAAAACGAGAAGA
AATTAAGGGTGTGCGTTATTCGTACCGTGGCATGAAGATGAAAGAGGAGCATATCGTTCCTCTCAGTCGGCAGGCGATGG
TTTTGTTAGAGCAGCTCAAGCAGATTAGTGGTGATAAAGAGCTGTTGTTTCCGGGGGATCACGACGCAACTAAGGTTATG
AGCGAAAACACGGTAAATAGCGCATTGCGTGCGATGGGTTATGACACAAAAACCGAGGTCTGCGGGCATGGGTTTCGGAC
GATGGCGCGTGGTGCTTTGGGTGAGTCAGGATTATGGAGCGATGACGCGATAGAGCGTCAGTTAAGCCATTCTGAGCGTA
ACAATGTGCGTGCTGCCTATATTCACACTTCAGAGCATTTGGATGAGCGTCGTTTGATGGTGCAGTGGTGGGCTGATTAT
CTCGATATTATTCAGTATGAGCATATTACACCTTATGAATATGCAAGAGTCTGCAACATAAAATGA

Upstream 100 bases:

>100_bases
TTTTTACGTCCTGAGTCGTCCGAGGTTGTCCGTTGAAATCCGGAAGTCATTGGGGGCATAATTGGGGGCATCTTAACTTC
GATTAGAAATGTGCCCCCAA

Downstream 100 bases:

>100_bases
ATGATATTTATAGATGATAATTTAATGGATTAAAATAGGCACAATTACTTGTGCCTATTAATTCAATCTTTCCAAAGAAT
TACAGGGTGTGCTCTAGGCC

Product: integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQG
IDPKTEQKEAQAENSGAYTFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LDIIQYEHITPYEYARVCNIK

Sequences:

>Translated_421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQG
IDPKTEQKEAQAENSGAYTFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LDIIQYEHITPYEYARVCNIK
>Mature_421_residues
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQG
IDPKTEQKEAQAENSGAYTFETIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDATKVM
SENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADY
LDIIQYEHITPYEYARVCNIK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=403, Percent_Identity=36.2282878411911, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788690, Length=402, Percent_Identity=34.0796019900498, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI145693166, Length=416, Percent_Identity=33.1730769230769, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48612; Mature: 48612

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPT
CCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEECCCH
VTLAQARAKRDEAKKLLVQGIDPKTEQKEAQAENSGAYTFETIAREWHASNKRWSEDHRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
RVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKHDVAQRLQQRVTAIMRYAVQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVPLSRQAMVLLEQLK
CCCCCEEECCCCCCCHHHHHCCCCHHHHCCCHHHHCCCCCCHHCCCCCCHHHHHHHHHHH
QISGDKELLFPGDHDATKVMSENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWS
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCC
DDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADYLDIIQYEHITPYEYARVCNI
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
K
C
>Mature Secondary Structure
MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRRPSDKKEDRLAFGVWPT
CCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEECCCH
VTLAQARAKRDEAKKLLVQGIDPKTEQKEAQAENSGAYTFETIAREWHASNKRWSEDHRS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
RVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKHDVAQRLQQRVTAIMRYAVQN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARLAAYRGRVMTRIAVELSLLTFVR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYRGMKMKEEHIVPLSRQAMVLLEQLK
CCCCCEEECCCCCCCHHHHHCCCCHHHHCCCHHHHCCCCCCHHCCCCCCHHHHHHHHHHH
QISGDKELLFPGDHDATKVMSENTVNSALRAMGYDTKTEVCGHGFRTMARGALGESGLWS
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCC
DDAIERQLSHSERNNVRAAYIHTSEHLDERRLMVQWWADYLDIIQYEHITPYEYARVCNI
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]