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The map label for this gene is tviC

Identifier: 29144602

GI number: 29144602

Start: 4504378

End: 4505424

Strand: Reverse

Name: tviC

Synonym: t4351

Alternate gene names: 29144602

Gene position: 4505424-4504378 (Counterclockwise)

Preceding gene: 29144603

Following gene: 29144601

Centisome position: 94.02

GC content: 42.5

Gene sequence:

>1047_bases
ATGACGGCTTACGAAGAACTACGGACCAAACTGGTTCTGGCACCAAAGCGCTGGCTGATCACTGGCGTAGCAGGCTTTAT
TGGCTCCGGCTTATTAGAAGAATTACTCTTTCTCAACCAGACTGTCATTGGACTGGATAACTTTTCCACCGGTTATCAGC
ATAATCTAGACGACGTTCGCACGTCCGTCAGTGAGGAGCAATGGTCGCGATTTATTTTTATTCAGGGTGACATCAGGAAA
TTTACTGACTGTCAGAAAGCGTGTAAGAACGTTGACTATGTTCTCCACCAAGCCGCGCTAGGAAGCGTGCCACGTTCCCT
AAAGGATCCCATCGCGACTAATAGCGCCAATATTGATGGTTTTTTAAATATGTTGACGGCGGCGAGAGATGCTCATGTCT
CTAGTTTCACCTACGCCGCAAGCAGTAGCACCTATGGAGACCATCCCGATTTACCTAAAATTGAGGAACGGATCGGTCGA
CCACTCAGCCCGTATGCGGTAACAAAATACGTCAATGAATTGTACGCTGATGTGTTTGCACGTAGCTATGAATTTAACGC
TATTGGCCTACGCTACTTTAATGTCTTTGGTCGCCGCCAAAATCCTAACGGAGCGTACTCGGCAGTTATTCCTCGCTGGA
TACTATCGCTTCTTAAAGATGAACCAATTTATATCAATGGCGATGGCTCAACAAGCAGGGATTTTTGCTATATAGAGAAT
GTGATTCAGGCCAATCTATTATCAGCAACAACTAATGATTTAGCCTCTAAAAATAAGGTCTATAATGTGGCAGTTGGAGA
TAGAACTTCGTTAAATGAGCTTTATTATCTAATTCGCGATGGGCTTAATTTATGGCGGAACGAACAAAGTAGAGCTGAAC
CAATTTATAAAGATTTTCGTGACGGTGACGTTAAGCATAGCCAGGCAGATATTACCAAAATAAAAACATTTCTTTCATAT
GAGCCTGAATTTGATATCAAAGAAGGACTTAAGCAGACTCTAAAATGGTATATCGATAAACATTCTACTTTGTATTCCTC
GGTATAA

Upstream 100 bases:

>100_bases
TGGGAAGTGAGGATATTCGCGGCTTCGGAAAAGATAAACATGTACTTTATGATTTGAAGTATGTTCTTCCGGCTGAGCAG
TCAGATGTGAGATTGTAATC

Downstream 100 bases:

>100_bases
CTACTCACTTTCCTTTCACGTGGATGAATTTAATGAAATCGTCAGGGATGTTTACGCTTACAGCCATTGGCAGTTGCCGT
ATTGTGAGTCCGGTAAAACG

Product: VI polysaccharide biosynthesis epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTAYEELRTKLVLAPKRWLITGVAGFIGSGLLEELLFLNQTVIGLDNFSTGYQHNLDDVRTSVSEEQWSRFIFIQGDIRK
FTDCQKACKNVDYVLHQAALGSVPRSLKDPIATNSANIDGFLNMLTAARDAHVSSFTYAASSSTYGDHPDLPKIEERIGR
PLSPYAVTKYVNELYADVFARSYEFNAIGLRYFNVFGRRQNPNGAYSAVIPRWILSLLKDEPIYINGDGSTSRDFCYIEN
VIQANLLSATTNDLASKNKVYNVAVGDRTSLNELYYLIRDGLNLWRNEQSRAEPIYKDFRDGDVKHSQADITKIKTFLSY
EPEFDIKEGLKQTLKWYIDKHSTLYSSV

Sequences:

>Translated_348_residues
MTAYEELRTKLVLAPKRWLITGVAGFIGSGLLEELLFLNQTVIGLDNFSTGYQHNLDDVRTSVSEEQWSRFIFIQGDIRK
FTDCQKACKNVDYVLHQAALGSVPRSLKDPIATNSANIDGFLNMLTAARDAHVSSFTYAASSSTYGDHPDLPKIEERIGR
PLSPYAVTKYVNELYADVFARSYEFNAIGLRYFNVFGRRQNPNGAYSAVIPRWILSLLKDEPIYINGDGSTSRDFCYIEN
VIQANLLSATTNDLASKNKVYNVAVGDRTSLNELYYLIRDGLNLWRNEQSRAEPIYKDFRDGDVKHSQADITKIKTFLSY
EPEFDIKEGLKQTLKWYIDKHSTLYSSV
>Mature_347_residues
TAYEELRTKLVLAPKRWLITGVAGFIGSGLLEELLFLNQTVIGLDNFSTGYQHNLDDVRTSVSEEQWSRFIFIQGDIRKF
TDCQKACKNVDYVLHQAALGSVPRSLKDPIATNSANIDGFLNMLTAARDAHVSSFTYAASSSTYGDHPDLPKIEERIGRP
LSPYAVTKYVNELYADVFARSYEFNAIGLRYFNVFGRRQNPNGAYSAVIPRWILSLLKDEPIYINGDGSTSRDFCYIENV
IQANLLSATTNDLASKNKVYNVAVGDRTSLNELYYLIRDGLNLWRNEQSRAEPIYKDFRDGDVKHSQADITKIKTFLSYE
PEFDIKEGLKQTLKWYIDKHSTLYSSV

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family

Homologues:

Organism=Homo sapiens, GI7657641, Length=331, Percent_Identity=29.3051359516616, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=28.1818181818182, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI56237023, Length=345, Percent_Identity=22.6086956521739, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI56118217, Length=345, Percent_Identity=22.6086956521739, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI189083684, Length=345, Percent_Identity=22.6086956521739, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=26.1627906976744, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=27.2463768115942, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=26.5536723163842, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=26.8072289156627, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=348, Percent_Identity=24.1379310344828, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=24.1379310344828, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=25.2199413489736, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=351, Percent_Identity=23.9316239316239, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=27.1641791044776, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=27.5071633237822, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIPB_SALTI (Q04973)

Other databases:

- EMBL:   X67785
- EMBL:   D14156
- EMBL:   AL627283
- EMBL:   AE014613
- PIR:   C36892
- RefSeq:   NP_458739.1
- RefSeq:   NP_807944.1
- ProteinModelPortal:   Q04973
- SMR:   Q04973
- GeneID:   1071186
- GeneID:   1250861
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t4351
- KEGG:   sty:STY4660
- HOGENOM:   HBG755066
- OMA:   HNLDEVQ
- ProtClustDB:   PRK15181
- BioCyc:   SENT209261:T4351-MONOMER
- BioCyc:   SENT220341:STY4660-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR01713

Pfam domain/function: PF01370 Epimerase

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 39503; Mature: 39372

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 165-165 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYEELRTKLVLAPKRWLITGVAGFIGSGLLEELLFLNQTVIGLDNFSTGYQHNLDDVR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCHHHHH
TSVSEEQWSRFIFIQGDIRKFTDCQKACKNVDYVLHQAALGSVPRSLKDPIATNSANIDG
HHHCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHH
FLNMLTAARDAHVSSFTYAASSSTYGDHPDLPKIEERIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RSYEFNAIGLRYFNVFGRRQNPNGAYSAVIPRWILSLLKDEPIYINGDGSTSRDFCYIEN
HCCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
VIQANLLSATTNDLASKNKVYNVAVGDRTSLNELYYLIRDGLNLWRNEQSRAEPIYKDFR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHC
DGDVKHSQADITKIKTFLSYEPEFDIKEGLKQTLKWYIDKHSTLYSSV
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAYEELRTKLVLAPKRWLITGVAGFIGSGLLEELLFLNQTVIGLDNFSTGYQHNLDDVR
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCHHHHH
TSVSEEQWSRFIFIQGDIRKFTDCQKACKNVDYVLHQAALGSVPRSLKDPIATNSANIDG
HHHCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHH
FLNMLTAARDAHVSSFTYAASSSTYGDHPDLPKIEERIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RSYEFNAIGLRYFNVFGRRQNPNGAYSAVIPRWILSLLKDEPIYINGDGSTSRDFCYIEN
HCCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
VIQANLLSATTNDLASKNKVYNVAVGDRTSLNELYYLIRDGLNLWRNEQSRAEPIYKDFR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHC
DGDVKHSQADITKIKTFLSYEPEFDIKEGLKQTLKWYIDKHSTLYSSV
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504