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The map label for this gene is tviB

Identifier: 29144603

GI number: 29144603

Start: 4505427

End: 4506704

Strand: Reverse

Name: tviB

Synonym: t4352

Alternate gene names: 29144603

Gene position: 4506704-4505427 (Counterclockwise)

Preceding gene: 29144604

Following gene: 29144602

Centisome position: 94.05

GC content: 43.97

Gene sequence:

>1278_bases
GTGTTCGGTATAGACGAGGTAAAAATCGCGATTATTGGGCTGGGATATGTTGGGCTTCCTCTGGCAGTTGAATTTGGCAA
ATCTCGTCAGGTTGTTGGCTTCGACGTTAATAAAAAGCGTATTCTTGAATTAAAGAATGGGGTGGATGTCAATCTGGAAA
CCACTGAAGAAGAATTACGTGAGGCTCGTTATCTGAAATTTACTTCCGAGATTGAGAAGATCAAAGAATGTAATTTTTAC
ATCATCACCGTCCCGACGCCGATAAATACCTACAAGCAACCAGACCTCACCCCACTAATCAAGGCGAGTGAAACCGTTGG
TACAGTGCTGAATCGGGGAGATATTGTGGTATATGAATCTACGGTATATCCGGGATGTACCGAAGAAGAATGCGTGCCGA
TCCTTGCTCGTATGTCCGGAATGACTTTCAACCAGGATTTCTATGTCGGTTATAGCCCGGAAAGGATCAATCCCGGTGAT
AAAAAGCACCGTTTAACCAACATCAAGAAAATCACCTCCGGTTCAACCGCACAGATCGCCGAACTTATCGATGAAGTATA
TCAGCAGATCATCAGCGCAGGTACATATAAAGCAGAGAGCATCAAAGTTGCTGAGGCAGCGAAGGTGATTGAAAATACGC
AACGCGATCTGAATATTGCCTTGGTCAATGAGCTGGCGATTATTTTTAATCGTTTAAATATCGATACTGAAGCCGTGCTA
CGTGCCGCTGGCAGCAAATGGAATTTCCTGCCATTCCGTCCGGGACTGGTCGGTGGTCACTGTATTGGCGTAGATCCCTA
TTATCTGACACATAAATCTCAGGGCATTGGCTATTATCCAGAAATCATACTTGCAGGACGCCGCCTGAACGACAACATGG
GCAACTATGTCTCCGAGCAGTTGATCAAAGCAATGATCAAAAAAGGAATTAACGTTGAGGGTTCCAGCGTGCTGATTCTC
GGCTTTACCTTTAAAGAAAACTGTCCGGACATCAGAAATACACGCATTATTGATGTGGTAAAGGAACTCGGTAAATATAG
TTGTAAAGTGGATATTTTTGATCCATGGGTGGATGCCGAAGAGGTAAGACGAGAGTATGGCATTATCCCGGTATCGGAAG
TCAAATCAAGCCACTACGATGCGATCATTGTTGCAGTAGGACATCAGCAATTTAAACAGATGGGAAGTGAGGATATTCGC
GGCTTCGGAAAAGATAAACATGTACTTTATGATTTGAAGTATGTTCTTCCGGCTGAGCAGTCAGATGTGAGATTGTAA

Upstream 100 bases:

>100_bases
TTAACCATACTGGCTGCTACGGCCAGGGGCGGTAGCGTATCTGAATAAACACCTAGAATTAACTTTGTAAATATAAAATT
TTAGTAAAGGATTAATAAGA

Downstream 100 bases:

>100_bases
TCATGACGGCTTACGAAGAACTACGGACCAAACTGGTTCTGGCACCAAAGCGCTGGCTGATCACTGGCGTAGCAGGCTTT
ATTGGCTCCGGCTTATTAGA

Product: VI polysaccharide biosynthesis UDP-glucose/GDP-mannose dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELREARYLKFTSEIEKIKECNFY
IITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYESTVYPGCTEEECVPILARMSGMTFNQDFYVGYSPERINPGD
KKHRLTNIKKITSGSTAQIAELIDEVYQQIISAGTYKAESIKVAEAAKVIENTQRDLNIALVNELAIIFNRLNIDTEAVL
RAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYPEIILAGRRLNDNMGNYVSEQLIKAMIKKGINVEGSSVLIL
GFTFKENCPDIRNTRIIDVVKELGKYSCKVDIFDPWVDAEEVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIR
GFGKDKHVLYDLKYVLPAEQSDVRL

Sequences:

>Translated_425_residues
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELREARYLKFTSEIEKIKECNFY
IITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYESTVYPGCTEEECVPILARMSGMTFNQDFYVGYSPERINPGD
KKHRLTNIKKITSGSTAQIAELIDEVYQQIISAGTYKAESIKVAEAAKVIENTQRDLNIALVNELAIIFNRLNIDTEAVL
RAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYPEIILAGRRLNDNMGNYVSEQLIKAMIKKGINVEGSSVLIL
GFTFKENCPDIRNTRIIDVVKELGKYSCKVDIFDPWVDAEEVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIR
GFGKDKHVLYDLKYVLPAEQSDVRL
>Mature_425_residues
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELREARYLKFTSEIEKIKECNFY
IITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYESTVYPGCTEEECVPILARMSGMTFNQDFYVGYSPERINPGD
KKHRLTNIKKITSGSTAQIAELIDEVYQQIISAGTYKAESIKVAEAAKVIENTQRDLNIALVNELAIIFNRLNIDTEAVL
RAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYPEIILAGRRLNDNMGNYVSEQLIKAMIKKGINVEGSSVLIL
GFTFKENCPDIRNTRIIDVVKELGKYSCKVDIFDPWVDAEEVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIR
GFGKDKHVLYDLKYVLPAEQSDVRL

Specific function: Catalyzes The Oxidation Of Udp-Mannac To Udp-N- Acetylmannosaminuronic Acid (Udp-Mannaca). [C]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family

Homologues:

Organism=Escherichia coli, GI48994968, Length=413, Percent_Identity=26.8765133171913, Blast_Score=152, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17136908, Length=386, Percent_Identity=27.720207253886, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIPA_SALTI (Q04972)

Other databases:

- EMBL:   X67785
- EMBL:   D14156
- EMBL:   AL627283
- EMBL:   AE014613
- PIR:   B36892
- RefSeq:   NP_458740.1
- RefSeq:   NP_807945.1
- ProteinModelPortal:   Q04972
- SMR:   Q04972
- GeneID:   1067316
- GeneID:   1250862
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t4352
- KEGG:   sty:STY4661
- HOGENOM:   HBG400967
- OMA:   LKFNVDF
- ProtClustDB:   PRK15182
- BioCyc:   SENT209261:T4352-MONOMER
- BioCyc:   SENT220341:STY4661-MONOMER
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11374
- PIRSF:   PIRSF000124
- TIGRFAMs:   TIGR03026

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N; SSF48179 6DGDH_C_like; SSF52413 UDP-Glc/GDP-Man_DH_C

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47675; Mature: 47675

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: ACT_SITE 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELR
CCCCCEEEEEEEEECHHCCCEEEECCCCCEEEEECCCHHHHHEECCCCEEEEECCHHHHH
EARYLKFTSEIEKIKECNFYIITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYES
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCEEEEEC
TVYPGCTEEECVPILARMSGMTFNQDFYVGYSPERINPGDKKHRLTNIKKITSGSTAQIA
CCCCCCCHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
ELIDEVYQQIISAGTYKAESIKVAEAAKVIENTQRDLNIALVNELAIIFNRLNIDTEAVL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYPEIILAGRRLNDNMGNYVSEQ
HHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHEEECCCCCCCHHHHHHHH
LIKAMIKKGINVEGSSVLILGFTFKENCPDIRNTRIIDVVKELGKYSCKVDIFDPWVDAE
HHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHH
EVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIRGFGKDKHVLYDLKYVLPAEQ
HHHHHHCCCCHHHHCCCCCCEEEEEECHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCCC
SDVRL
CCCCC
>Mature Secondary Structure
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELR
CCCCCEEEEEEEEECHHCCCEEEECCCCCEEEEECCCHHHHHEECCCCEEEEECCHHHHH
EARYLKFTSEIEKIKECNFYIITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYES
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCEEEEEC
TVYPGCTEEECVPILARMSGMTFNQDFYVGYSPERINPGDKKHRLTNIKKITSGSTAQIA
CCCCCCCHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
ELIDEVYQQIISAGTYKAESIKVAEAAKVIENTQRDLNIALVNELAIIFNRLNIDTEAVL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYPEIILAGRRLNDNMGNYVSEQ
HHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHEEECCCCCCCHHHHHHHH
LIKAMIKKGINVEGSSVLILGFTFKENCPDIRNTRIIDVVKELGKYSCKVDIFDPWVDAE
HHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHH
EVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIRGFGKDKHVLYDLKYVLPAEQ
HHHHHHCCCCHHHHCCCCCCEEEEEECHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCCC
SDVRL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504