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The map label for this gene is pilQ [H]

Identifier: 29144502

GI number: 29144502

Start: 4413760

End: 4415475

Strand: Direct

Name: pilQ [H]

Synonym: t4245

Alternate gene names: 29144502

Gene position: 4413760-4415475 (Clockwise)

Preceding gene: 29144501

Following gene: 29144503

Centisome position: 92.11

GC content: 54.55

Gene sequence:

>1716_bases
ATGTCTGAACACAACGATGCGGTTCTGCTTTACCACAACCCGGACACCACGATCCTCGAGCTGCTGGTTGACAGCCAGCG
GCGTACCGACGTGGCGGTGCAGCAGAAGGTAATGTCTCTGATGGAGGAAAACCCCGGTGTCACAATGAGGCTGGTAACCC
GTTCAGAGTTGCTCAGGTACAGTGAAATGGCTCGCCAGCAGTCAGAGAAGGGAGAAAAAGGACTTCGCCTTGAGGACATC
AAAAAAGAGGTCAGCAGCCGTCAGGGCGATGTTCTCAACTATTTTATGGTCGCGAAAGATATCGGCGCGTCTGATATTCA
CGGTACCACCCGGCCAGGACTGACGCGCATCGAGATGCGTGTGCACGGTGAACTGGAGACCGTTTCGGAACTGGCTGAAG
AAGACGGAAAAGCACAGGCGGCCACTATCATCCTTTCCATGTGTGATGTGACCGAACCCCAGTTCTACGCCGGCCGAAAA
CAGGATGGTCGTGTGGCTGCAAAGTTTCTGCGAAAGGTGGGGTTATTTGGCGCCCGTTACAGCCACACCCCGACAGCAGA
TGGCCTTCACTTTGTGATGCGCGTGATCCCGGATGATGGTGATGTGGTTCCCACGCTCGAAAAACTCGGTTTTCTGCCGA
AACAAATCCGCCTGATAAAGCGCACTTTGCGCCTGCCAGAAGGGATGATTATTTTGTCCGGCCCGACCGGAGCCGGGAAA
TCCACCACCCTGCGTGCCTGCAGCGACCTCTATCTGAAGCGTACCCGGTATAAAAAGCGCCTACTGACGGTTGAAGATCC
GCCGGAGGGACGCATCGTTGGGGCAATACAGACTCCTATTATCTGTGACAAAGCCGATGAGGCGGAAGTCAGGCTGGCCT
GGCAGCGGGTGCTGACCTCAGCGTTACGTCTCGATCCGGATGCCATTTTACCTGGTGAACTTCGTGACCTTATCTCCATT
CTGGCCGGGATTTTTGCCGCCCAGACCGGCCATCTGGTGCTGAGTACCCTGCATTCAAACTCGGCCTTTTCCATCCCTGA
GCGAATGATAACGATGGGTGCAAATGCCGGACTGGTGGCAGATGCCCAGTTGTTGGTCGGGCTTATCAGCCAGCGTCTGG
TTCAGGTGCTGTGTCCCGAGTGCCGTATTCCCTGGTCGGTTAAAAAAGAACATCTCACCACCGAAGAACGTGAATATCTG
GAAAAGTACTGTTCAGTGGAGGGGGTTTGTCAGCCCGAAAATCTTCATTTCAGAAATGAGGAGGGGTGCCCGACCTGCCG
GAAGGCGGTCATTATCGGTGGCAAACAGGTGGCAACAGTCGGTAAGGGGGTGGCCGGACGCAGTGTGGTGGCGGAGGTGG
TTCGCACGGATTCTACGCTTTTCAGGCTGTTATTACAGCAGGGTAAGGAAGCTGCAAAAGCTTACTGGATCCGTGAAATG
GGGGGGATTACCCGTCGCATTCATCTGCTTCACAAACTGAATGACGGGCTGGTTGACCCACTGGATGCCGATTTGATTAT
TCCTCTTGATGAGGATGAGGTGATGCAGGTGGTAAGTGATCAGGTTGCATCGTCGAGTGACATTGATACGTCGCTGATTG
AACGCCTTGCCATGGTAATTGCAGCCAGCGTCAGAGAGGGGATGAAGCAGGGTGTTGAGGAAGCCATTAAAGAAGGCGTT
GCTGCCGGGATGGCTGCGCTGAAGGAGGTTGCATGA

Upstream 100 bases:

>100_bases
GGGAAGTGTGATTCCGGGGACATCACTGAAGGTGAAGGCAATAACGCTCTCATCCGTCACGCTCAGTGACGGTCAGCAAC
TGACGTTTTAGGGACGAATT

Downstream 100 bases:

>100_bases
GTCCTTTATACCGTCTTCGCCGGGCGCTGGCGCGACGTTTTTTTACGTCCGGATACCGTCTGCGTTTTTACCGCATGCTG
TCGTTGCAGATGAGAAATGG

Product: nucleotide-binding protein

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpT [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSEHNDAVLLYHNPDTTILELLVDSQRRTDVAVQQKVMSLMEENPGVTMRLVTRSELLRYSEMARQQSEKGEKGLRLEDI
KKEVSSRQGDVLNYFMVAKDIGASDIHGTTRPGLTRIEMRVHGELETVSELAEEDGKAQAATIILSMCDVTEPQFYAGRK
QDGRVAAKFLRKVGLFGARYSHTPTADGLHFVMRVIPDDGDVVPTLEKLGFLPKQIRLIKRTLRLPEGMIILSGPTGAGK
STTLRACSDLYLKRTRYKKRLLTVEDPPEGRIVGAIQTPIICDKADEAEVRLAWQRVLTSALRLDPDAILPGELRDLISI
LAGIFAAQTGHLVLSTLHSNSAFSIPERMITMGANAGLVADAQLLVGLISQRLVQVLCPECRIPWSVKKEHLTTEEREYL
EKYCSVEGVCQPENLHFRNEEGCPTCRKAVIIGGKQVATVGKGVAGRSVVAEVVRTDSTLFRLLLQQGKEAAKAYWIREM
GGITRRIHLLHKLNDGLVDPLDADLIIPLDEDEVMQVVSDQVASSSDIDTSLIERLAMVIAASVREGMKQGVEEAIKEGV
AAGMAALKEVA

Sequences:

>Translated_571_residues
MSEHNDAVLLYHNPDTTILELLVDSQRRTDVAVQQKVMSLMEENPGVTMRLVTRSELLRYSEMARQQSEKGEKGLRLEDI
KKEVSSRQGDVLNYFMVAKDIGASDIHGTTRPGLTRIEMRVHGELETVSELAEEDGKAQAATIILSMCDVTEPQFYAGRK
QDGRVAAKFLRKVGLFGARYSHTPTADGLHFVMRVIPDDGDVVPTLEKLGFLPKQIRLIKRTLRLPEGMIILSGPTGAGK
STTLRACSDLYLKRTRYKKRLLTVEDPPEGRIVGAIQTPIICDKADEAEVRLAWQRVLTSALRLDPDAILPGELRDLISI
LAGIFAAQTGHLVLSTLHSNSAFSIPERMITMGANAGLVADAQLLVGLISQRLVQVLCPECRIPWSVKKEHLTTEEREYL
EKYCSVEGVCQPENLHFRNEEGCPTCRKAVIIGGKQVATVGKGVAGRSVVAEVVRTDSTLFRLLLQQGKEAAKAYWIREM
GGITRRIHLLHKLNDGLVDPLDADLIIPLDEDEVMQVVSDQVASSSDIDTSLIERLAMVIAASVREGMKQGVEEAIKEGV
AAGMAALKEVA
>Mature_570_residues
SEHNDAVLLYHNPDTTILELLVDSQRRTDVAVQQKVMSLMEENPGVTMRLVTRSELLRYSEMARQQSEKGEKGLRLEDIK
KEVSSRQGDVLNYFMVAKDIGASDIHGTTRPGLTRIEMRVHGELETVSELAEEDGKAQAATIILSMCDVTEPQFYAGRKQ
DGRVAAKFLRKVGLFGARYSHTPTADGLHFVMRVIPDDGDVVPTLEKLGFLPKQIRLIKRTLRLPEGMIILSGPTGAGKS
TTLRACSDLYLKRTRYKKRLLTVEDPPEGRIVGAIQTPIICDKADEAEVRLAWQRVLTSALRLDPDAILPGELRDLISIL
AGIFAAQTGHLVLSTLHSNSAFSIPERMITMGANAGLVADAQLLVGLISQRLVQVLCPECRIPWSVKKEHLTTEEREYLE
KYCSVEGVCQPENLHFRNEEGCPTCRKAVIIGGKQVATVGKGVAGRSVVAEVVRTDSTLFRLLLQQGKEAAKAYWIREMG
GITRRIHLLHKLNDGLVDPLDADLIIPLDEDEVMQVVSDQVASSSDIDTSLIERLAMVIAASVREGMKQGVEEAIKEGVA
AGMAALKEVA

Specific function: Involved in the translocation of the tcpA pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=334, Percent_Identity=32.0359281437126, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=34.8754448398576, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI87082188, Length=163, Percent_Identity=38.0368098159509, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62900; Mature: 62768

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHNDAVLLYHNPDTTILELLVDSQRRTDVAVQQKVMSLMEENPGVTMRLVTRSELLRY
CCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
SEMARQQSEKGEKGLRLEDIKKEVSSRQGDVLNYFMVAKDIGASDIHGTTRPGLTRIEMR
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
VHGELETVSELAEEDGKAQAATIILSMCDVTEPQFYAGRKQDGRVAAKFLRKVGLFGARY
ECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCC
SHTPTADGLHFVMRVIPDDGDVVPTLEKLGFLPKQIRLIKRTLRLPEGMIILSGPTGAGK
CCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
STTLRACSDLYLKRTRYKKRLLTVEDPPEGRIVGAIQTPIICDKADEAEVRLAWQRVLTS
CHHHHHHHHHHHHHHHHHHHCEEECCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHH
ALRLDPDAILPGELRDLISILAGIFAAQTGHLVLSTLHSNSAFSIPERMITMGANAGLVA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCHHHHHHHCCCCCCHH
DAQLLVGLISQRLVQVLCPECRIPWSVKKEHLTTEEREYLEKYCSVEGVCQPENLHFRNE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
EGCPTCRKAVIIGGKQVATVGKGVAGRSVVAEVVRTDSTLFRLLLQQGKEAAKAYWIREM
CCCCHHHHEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
GGITRRIHLLHKLNDGLVDPLDADLIIPLDEDEVMQVVSDQVASSSDIDTSLIERLAMVI
CCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AASVREGMKQGVEEAIKEGVAAGMAALKEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEHNDAVLLYHNPDTTILELLVDSQRRTDVAVQQKVMSLMEENPGVTMRLVTRSELLRY
CCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
SEMARQQSEKGEKGLRLEDIKKEVSSRQGDVLNYFMVAKDIGASDIHGTTRPGLTRIEMR
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
VHGELETVSELAEEDGKAQAATIILSMCDVTEPQFYAGRKQDGRVAAKFLRKVGLFGARY
ECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCC
SHTPTADGLHFVMRVIPDDGDVVPTLEKLGFLPKQIRLIKRTLRLPEGMIILSGPTGAGK
CCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
STTLRACSDLYLKRTRYKKRLLTVEDPPEGRIVGAIQTPIICDKADEAEVRLAWQRVLTS
CHHHHHHHHHHHHHHHHHHHCEEECCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHH
ALRLDPDAILPGELRDLISILAGIFAAQTGHLVLSTLHSNSAFSIPERMITMGANAGLVA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCHHHHHHHCCCCCCHH
DAQLLVGLISQRLVQVLCPECRIPWSVKKEHLTTEEREYLEKYCSVEGVCQPENLHFRNE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
EGCPTCRKAVIIGGKQVATVGKGVAGRSVVAEVVRTDSTLFRLLLQQGKEAAKAYWIREM
CCCCHHHHEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
GGITRRIHLLHKLNDGLVDPLDADLIIPLDEDEVMQVVSDQVASSSDIDTSLIERLAMVI
CCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AASVREGMKQGVEEAIKEGVAAGMAALKEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8097178; 10952301 [H]