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The map label for this gene is melB [H]

Identifier: 29144468

GI number: 29144468

Start: 4373604

End: 4375034

Strand: Direct

Name: melB [H]

Synonym: t4206

Alternate gene names: 29144468

Gene position: 4373604-4375034 (Clockwise)

Preceding gene: 29144467

Following gene: 29144474

Centisome position: 91.27

GC content: 52.62

Gene sequence:

>1431_bases
ATGAGCATCTCTCTGACAACAAAGCTGAGTTACGGGTTCGGTGCGTTTGGTAAGGATTTCGCCATCGGCATTGTGTATAT
GTACCTGATGTATTACTACACCGATGTGGTGGGACTCTCGGTCGGCCTCGTCGGCACCCTCTTTCTGGTCGCGCGAATTT
GGGATGCGATAAACGATCCCATCATGGGCTGGATTGTCAACGCCACGCGTTCGCGGTGGGGGAAATTTAAGCCGTGGATA
TTGATCGGCACCTTAACCAATTCGCTGGTGCTTTTCCTGCTGTTCAGCGCCCATCTTTTTGAGGGAACCGCGCAGGTTGT
ATTTGTCTGCGTAACCTACATCCTGTGGGGCATGACGTATACCATTATGGATATCCCATTTTGGTCGCTGGTGCCGACCA
TTACGCTTGATAAGCGAGAACGCGAACAACTGGTGCCGTTCCCGCGTTTCTTCGCCAGTCTGGCTGGCTTCGTCACTGCC
GGTATAACGCTGCCGTTTGTGAACTATGTTGGCGGGGCGGATCGTGGGTTCGGCTTTCAGATGTTTACGCTGGTACTGAT
TGCGTTCTTTATCGCCTCGACTATCGTGACATTACGCAACGTACATGAGGTGTACTCCTCCGACAACGGTGTAACGGCGG
GCCGCCCACATCTGACGTTAAAAACGATCGTTGGATTGATATACAAAAACGATCAGCTCTCTTGCCTGTTGGGAATGGCG
CTGGCGTATAACATCGCCTCCAATATTATCAATAGCTTTGCGATCTACTACTTCACCTATGTGATTGGCGATGCCGATCT
TTTTCCCTATTACCTTTCTTACGCCGGCGCGGCGAATCTGCTGACGCTGATTGTCTTCCCCCGGCTGGTGAAAATGTTAT
CGCGGCGGATATTGTGGGCGGGCGCCTCCGTGATGCCCGTTCTGAGTTGCGCAGGGCTCTTCGCGATGGCGTTGGCGGAT
GTCCATAATGCCGCTTTAATCGTGGCGGCGGGTATTTTCCTGAATATCGGGACCGCGCTCTTTTGGGTGCTTCAGGTAAT
CATGGTGGCGGATACGGTCGATTATGGGGAATTTAAGCTCAATATTCGCTGCGAGAGTATCGCTTATTCCGTACAGACGA
TGGTTGTGAAGGGCGGCTCGGCGTTTGCGGCGTTCTTTATCGCTCTGGTGCTGGGGCTGATTGGCTACACGCCGAACGTG
GCGCAGTCTGCGCAAACCCTGCAGGGGATGCAGTTTATTATGATTGTCCTGCCGGTACTGTTTTTCATGATGACGTTGGT
TCTCTACTTCCGCTACTACCGTTTGAACGGCGATATGCTGCGCAAGATTCAGATCCACCTGCTGGATAAATACCGGAAAA
CGCCGCCATTCGTCGAACAGCCGGATAGCCCGGCGATTTCTGTGGTAGCGACCAGCGATGTAAAAGCGTGA

Upstream 100 bases:

>100_bases
CCTGGTTACGCCGTTAATCTCCAGCCGCGCTGGACGGCAGATCCGGCGTTTAACCTCTACTAACAGAAGGCGGGCGCTGG
TAACAGCGACCCGGTACCCT

Downstream 100 bases:

>100_bases
GCATGGTCTCTCGTGCAGGCCGGATAAGCCGGATAAGCGTAGCGCCATCCGGCAATTAACCGATAACGCGGCCCGGGAGA
ACGCCGGGCCTGCCAGGTTA

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTA
GITLPFVNYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
LAYNIASNIINSFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALAD
VHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNV
AQSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA

Sequences:

>Translated_476_residues
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTA
GITLPFVNYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
LAYNIASNIINSFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALAD
VHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNV
AQSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
>Mature_475_residues
SISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWIL
IGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAG
ITLPFVNYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMAL
AYNIASNIINSFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALADV
HNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVA
QSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA

Specific function: Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=472, Percent_Identity=84.7457627118644, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=462, Percent_Identity=29.004329004329, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1786466, Length=459, Percent_Identity=27.0152505446623, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI48994989, Length=476, Percent_Identity=25.2100840336134, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693206, Length=465, Percent_Identity=25.8064516129032, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787902, Length=463, Percent_Identity=25.2699784017279, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52844; Mature: 52713

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IMGWIVNATRSRWGKFKPWILIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTY
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAGITLPFVNYVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAYNIASNIINSFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASVMPVLSCAGLFAMALADVHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVAQSAQTLQGMQFIMIVLPVL
EEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IMGWIVNATRSRWGKFKPWILIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTY
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAGITLPFVNYVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAYNIASNIINSFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASVMPVLSCAGLFAMALADVHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVAQSAQTLQGMQFIMIVLPVL
EEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1495487; 11677609 [H]