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The map label for this gene is melA [H]

Identifier: 29144467

GI number: 29144467

Start: 4372168

End: 4373520

Strand: Direct

Name: melA [H]

Synonym: t4205

Alternate gene names: 29144467

Gene position: 4372168-4373520 (Clockwise)

Preceding gene: 29144459

Following gene: 29144468

Centisome position: 91.24

GC content: 55.88

Gene sequence:

>1353_bases
ATGACGGCACCCAAAATTACCTTTATCGGCGCAGGTTCTACGATTTTCGTCAAAAATATCCTCGGCGATGTGTTTCACCG
CGAGGCGCTAAAGTCAGCGCATGTCGCCCTGATGGATATTGACGAAACCCGGCTGGAAGAGTCGCACATTGTGGTACGGA
AACTGATGGACTCAGCGGGCGCTTCTGGCCGGATTACCTGCCATACCCACCAGAAAGCGGCGCTACAGGATGCGGATTTC
GTGGTGGTCGCCTTTCAGATTGGCGGCTATGAACCCTGCACCGTGACCGATTTTGAGGTTTGTAAGCGTCATGGCCTGGA
ACAGACGATCGCCGATACGCTGGGGCCGGGCGGCATCATGCGCGCGCTGCGAACCATCCCGCATCTGTGGCAGATTTGCG
AAGACATGACGGAAGTCTGTCCGAAGGCCACCATGCTCAATTACGTCAACCCGATGGCGATGAATACCTGGGCGATGTAT
GCCCGCTATCCGCATATCAAACAGGTCGGCCTGTGCCATTCGGTACAGGGAACGGCGGAAGAACTGGCGCGCGACTTGAA
TATCGATCCCGCCTCGCTGCGCTACCGCTGCGTCGGCATTAACCACATGGCGTTTTACCTGGAACTGGAGCGCAAAACGG
CTGACGGGACTTATGTCAATCTCTATCCTGAATTGCTGGCGGCTTATGACGCCGGACAGGCGCCGAAGCCCAATATTCAC
GGCAATGAACGCTGCCAGAACATCGTGCGCTATGAGATGTTCAAAAAGTTGGGCTACTTCGTCACGGAGTCATCAGAGCA
TTTTGCCGAGTACACGCCGTGGTTTATTAAACCGGGACGCGAGGATCTGATTGCGCGCTACAAGGTGCCGCTGGATGAAT
ATCCGAAACGCTGCGTAGAACAACTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAACCGCCGAGCGTATCGACATC
AAACCGTCCCGCGAGTACGCCAGCACCATTATGAACGCTCTGTGGACCGGCGAGCCGAGCGTGATTTACGGCAATGTGCG
TAATGAGGGACTGATTGATAACCTGCCGCAGGGAAGCTGCGTCGAGGTGGCTTGTCTGGTAGATGCCAACGGCATTCAAC
CGACGAAGGTGGGGACGATCCCTTCCCATCTGGCGGCAATGATGCAGACCAACATCAACGTGCAAACGCTGTTGACCGAA
GCCATCCTCACGGAAAACCGCGATCGCGTGTATCACGCGGCGATGATGGACCCTCATACCGCGGCGGTGCTGGGTATCGA
AGAAATCTATGCGTTGGTTGACGATCTGATCGCCGCGCATGGCGACTGGCTTCCGGCCTGGTTACGCCGTTAA

Upstream 100 bases:

>100_bases
ACGATCTGAGTTTTCGGGAATGCTCATCGGGGAAGACGGAGGTTTTCACCGGGAACGCGTGCCACCATGAAGGTGTTCAC
TACTGTTCTGGAGAGTCATG

Downstream 100 bases:

>100_bases
TCTCCAGCCGCGCTGGACGGCAGATCCGGCGTTTAACCTCTACTAACAGAAGGCGGGCGCTGGTAACAGCGACCCGGTAC
CCTATGAGCATCTCTCTGAC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTHQKAALQDADF
VVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPKATMLNYVNPMAMNTWAMY
ARYPHIKQVGLCHSVQGTAEELARDLNIDPASLRYRCVGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNIH
GNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERIDI
KPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTE
AILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR

Sequences:

>Translated_450_residues
MTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTHQKAALQDADF
VVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPKATMLNYVNPMAMNTWAMY
ARYPHIKQVGLCHSVQGTAEELARDLNIDPASLRYRCVGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNIH
GNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERIDI
KPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTE
AILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR
>Mature_449_residues
TAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTHQKAALQDADFV
VVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPKATMLNYVNPMAMNTWAMYA
RYPHIKQVGLCHSVQGTAEELARDLNIDPASLRYRCVGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNIHG
NERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERIDIK
PSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTEA
ILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=450, Percent_Identity=94.4444444444444, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=455, Percent_Identity=24.8351648351648, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50598; Mature: 50466

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAG
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCC
ASGRITCHTHQKAALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIM
CCCEEEEECCCHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHHH
RALRTIPHLWQICEDMTEVCPKATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTAE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHHH
ELARDLNIDPASLRYRCVGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNIH
HHHHHCCCCHHHCEEEEEEECEEEEEEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCCC
GNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVE
CCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHHH
QLANWHKELEEYKTAERIDIKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSC
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
VEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTEAILTENRDRVYHAAMMDPHT
EEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCH
AAVLGIEEIYALVDDLIAAHGDWLPAWLRR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure 
TAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCC
ASGRITCHTHQKAALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIM
CCCEEEEECCCHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHHH
RALRTIPHLWQICEDMTEVCPKATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTAE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHHH
ELARDLNIDPASLRYRCVGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNIH
HHHHHCCCCHHHCEEEEEEECEEEEEEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCCC
GNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVE
CCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHHH
QLANWHKELEEYKTAERIDIKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSC
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
VEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTEAILTENRDRVYHAAMMDPHT
EEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCH
AAVLGIEEIYALVDDLIAAHGDWLPAWLRR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]