Click here to switch to the map view.

The map label for this gene is gntT [H]

Identifier: 29144262

GI number: 29144262

Start: 4143520

End: 4144836

Strand: Reverse

Name: gntT [H]

Synonym: t3994

Alternate gene names: 29144262

Gene position: 4144836-4143520 (Counterclockwise)

Preceding gene: 29144263

Following gene: 29144259

Centisome position: 86.5

GC content: 55.96

Gene sequence:

>1317_bases
ATGCCATTAGTCATCGTTGCTATCGGTGTTATCTTGTTACTTCTTCTGATGATCCGCTTCAAAATGAACGGATTTATCGC
CCTTGTCCTGGTAGCGCTCGCTGTCGGGTTGATGCAGGGAATGCCGCTGGATAAAGTCATCGGCTCCATCAAAGCCGGTG
TCGGCGGTACGCTTGGCAGTCTGGCCCTGATTATGGGGTTCGGCGCCATGCTGGGCAAAATGCTTGCCGACTGCGGCGGC
GCTCAACGTATCGCCACTACCCTGATCGCGAAATTTGGTAAAAAGCATATTCAGTGGGCCGTAGTGTTAACCGGCTTTAC
CGTCGGCTTCGCTCTGTTCTATGAAGTGGGCTTCGTTCTGATGCTGCCGCTGGTATTCACCATTGCCGCAGCGGCGAATA
TCCCGTTACTGTACGTCGGCGTGCCGATGGCCGCGGCGCTCTCCGTCACACACGGCTTCCTGCCGCCGCACCCGGGTCCA
ACCGCGATCGCCACGATTTTCCATGCCGATATGGGTAAAACGCTGCTGTTTGGTACGATTCTGGCGATCCCGACCGTGAT
CCTTGCCGGACCGGTTTACGCCCGTTTTCTGAAAGGCATCGATAAGCCGATCCCGGAAGGCCTGTACAGCGCGAAAACCT
TTACGGAAGAAGAGATGCCGGGCTTTGGCGTCAGCGTCTGGACGTCGCTGGTTCCGGTGATTCTGATGGCGATGCGTGCG
GTTGCCGAGATGATTCTGCCAAAAGGCCACGCTTTCCTGCCCGTTGCCGAGTTCCTGGGCGACCCGGTAATGGCTACGTT
GATTGCCGTACTGATTGCAATGTTCACCTTTGGCCTGAACCGCGGGCGTTCAATGGATCAGATTAACGATACGCTGGTCT
CTTCCATCAAAATTATCGCCATGATGCTGCTGATCATCGGCGGCGGCGGCGCGTTCAAGCAGGTGCTGGTCGATAGCGGC
GTGGATAAATACATCGCTTCAATGATGCATGAAACCAATGTCTCTCCGCTGTTGATGGCGTGGTCTATCGCGGCGGTGCT
GCGTATCGCGCTGGGCTCTGCGACTGTCGCGGCGATTACCGCAGGCGGCATTGCGGCGCCACTGATCGCCACGACCGGCG
TTAGTCCTGAACTGATGGTTATCGCAGTCGGTTCCGGGAGCGTGATTTTCTCTCACGTCAACGACCCAGGCTTCTGGCTG
TTCAAAGAGTATTTCAATTTGACTATCGGCGAGACCATTAAATCCTGGTCGATGCTGGAAACGATCATCTCCGTCTGCGG
CCTGATCGGCTGTCTGCTACTGGGTATGGTGGTTTAA

Upstream 100 bases:

>100_bases
CAGTATTGGTGCTATTGCGACTCTGTTCCCGTCGGGAACAACAGGAATTTGTCGTCGAAACAATGACGTTACCCATAACA
AAGTAAAGGCCAGGTAAATC

Downstream 100 bases:

>100_bases
GCGAGGCGGTTTGCAGGCCGGATAAGGCAGCAATGTGCGCCAGAATTGCCTGATGGCGCTGCGCTTATCAGGCCTACGTG
CTCTCACCATCATAAAGCCG

Product: high-affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGG
AQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAAAANIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFHADMGKTLLFGTILAIPTVILAGPVYARFLKGIDKPIPEGLYSAKTFTEEEMPGFGVSVWTSLVPVILMAMRA
VAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASMMHETNVSPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIKSWSMLETIISVCGLIGCLLLGMVV

Sequences:

>Translated_438_residues
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGG
AQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAAAANIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFHADMGKTLLFGTILAIPTVILAGPVYARFLKGIDKPIPEGLYSAKTFTEEEMPGFGVSVWTSLVPVILMAMRA
VAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASMMHETNVSPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIKSWSMLETIISVCGLIGCLLLGMVV
>Mature_437_residues
PLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGA
QRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAAAANIPLLYVGVPMAAALSVTHGFLPPHPGPT
AIATIFHADMGKTLLFGTILAIPTVILAGPVYARFLKGIDKPIPEGLYSAKTFTEEEMPGFGVSVWTSLVPVILMAMRAV
AEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGV
DKYIASMMHETNVSPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLF
KEYFNLTIGETIKSWSMLETIISVCGLIGCLLLGMVV

Specific function: Part of the gluconate utilization system Gnt-I; high- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=97.2602739726027, Blast_Score=821, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=438, Percent_Identity=61.1872146118721, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=49.2204899777283, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790776, Length=438, Percent_Identity=40.4109589041096, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788707, Length=440, Percent_Identity=36.1363636363636, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI48994939, Length=434, Percent_Identity=36.1751152073733, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1789097, Length=442, Percent_Identity=34.841628959276, Blast_Score=228, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 45919; Mature: 45788

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGS
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFTIAAAANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFHADMGKTLLFGTI
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
LAIPTVILAGPVYARFLKGIDKPIPEGLYSAKTFTEEEMPGFGVSVWTSLVPVILMAMRA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHH
VAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIA
HHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNVSPLLMAWSIAAVLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIKSWSMLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIISVCGLIGCLLLGMVV
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFTIAAAANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFHADMGKTLLFGTI
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
LAIPTVILAGPVYARFLKGIDKPIPEGLYSAKTFTEEEMPGFGVSVWTSLVPVILMAMRA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHH
VAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIA
HHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNVSPLLMAWSIAAVLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIKSWSMLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIISVCGLIGCLLLGMVV
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 2845225; 7984428; 3040894; 9045817 [H]