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The map label for this gene is dctA [H]

Identifier: 29144175

GI number: 29144175

Start: 4036508

End: 4037794

Strand: Direct

Name: dctA [H]

Synonym: t3903

Alternate gene names: 29144175

Gene position: 4036508-4037794 (Clockwise)

Preceding gene: 29144174

Following gene: 29144176

Centisome position: 84.23

GC content: 53.54

Gene sequence:

>1287_bases
ATGAAAACCTCTCTGTTCAAAAGTCTTTATTTTCAGGTCCTGACAGCAATCGCCATTGGTATTCTCCTTGGCCATTACTA
TCCTGAACTGGGCGCACAAATGAAACCGCTTGGCGACGCGTTCGTTAAGCTCATTAAGATGATCATCGCTCCTGTCATTT
TCTGTACTGTCGTGACGGGCATCGCAGGCATGGAAAGCATGAAAGCGGTGGGGCGTACCGGCGCGGTCGCGCTGCTTTAC
TTTGAAATTGTCAGTACGATTGCGCTGATTAACGGTCTTATCATCGTCAACGTTGTGCAACCTGGCGCCGGGATGAACGT
CGATCCGGCAACGCTGGATGCGCAGGCGGTGGCCGTTTATGCCGCACAAGCCAAGGAGCAGGGCATTATTGCCTTCCTGA
TGGATGTCATACCGGGCAGCGTGATCGGCGCGTTTGCCAGCGGCAACATTCTGCAGGTCTTACTGTTTGCGGTGTTGTTT
GGTTTTGCGCTGCACCGTTTGGGCAGCAAAGGCCAGCTCATTTTCAATGTGATTGAGAGTTTTTCGCAGGTCATTTTCGG
CATCATCAATATGATCATGCGCCTGGCGCCGATTGGCGCATTCGGCGCGATGGCCTTCACCATCGGTAAATACGGCGTCG
GTTCTCTGGTGCAACTGGGGCAGCTCATTATCTGCTTCTATATCACCTGTATTCTCTTCGTGGTGGTGGTGCTGGGGACG
ATTGCACGGGTAACGGGCTTTAGCATTTTTAAATTTATTCGCTATATCCGGGAAGAATTACTCATTGTGCTCGGCACCTC
CTCTTCCGAGTCAGCGTTGCCGCGTATGCTCGATAAGATGGAAAAACTGGGGTGCCGTAAATCGGTGGTGGGGCTGGTGA
TCCCGACGGGGTATTCGTTCAACCTTGATGGCACCTCTATCTACCTGACGATGGCGGCGGTGTTTATCGCCCAGGCGACC
AACAGCCATATGGATATCTTCCACCAGATCACGTTGCTGGTCGTGCTGCTGCTATCGTCAAAAGGCGCCGCGGGCGTGAC
CGGAAGCGGCTTTATCGTGCTGGCGGCCACTATCTCCGCCGTAGGTCATTTGCCGGTGGCGGGACTGGCGTTAATCCTCG
GTATCGACCGCTTTATGTCCGAAGCGCGCGCGCTGACTAACCTGGTGGGTAACGGCGTGGCGACGGTGGTGGTTGCCAAA
TGGGTGAAAGAGTTAGATCACCAAAAGCTGGACGATGTGCTTAATAATCGTGCGCCGGATGGCAAAACGCACGAAATTTC
CTCCTAA

Upstream 100 bases:

>100_bases
GATCCTGGCGCATTTCTGTCATGTTGTGTGGGTGTTATTTTAAGGCCGCAGGTTACTCATAACCTTACAAGACCTGTGGT
TTTTACTTCAAGGACACCCT

Downstream 100 bases:

>100_bases
TCTCGCCACTTATGCCCGTAGTCCCTTCCAGGACTGCGGGTGTTTGCGCATAATTGACCCCTGCGCCCAAAGCATACAGC
ATGATGCTTAGGGATTATTT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKTSLFKSLYFQVLTAIAIGILLGHYYPELGAQMKPLGDAFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALINGLIIVNVVQPGAGMNVDPATLDAQAVAVYAAQAKEQGIIAFLMDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICFYITCILFVVVVLGT
IARVTGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDHQKLDDVLNNRAPDGKTHEISS

Sequences:

>Translated_428_residues
MKTSLFKSLYFQVLTAIAIGILLGHYYPELGAQMKPLGDAFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALINGLIIVNVVQPGAGMNVDPATLDAQAVAVYAAQAKEQGIIAFLMDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICFYITCILFVVVVLGT
IARVTGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDHQKLDDVLNNRAPDGKTHEISS
>Mature_428_residues
MKTSLFKSLYFQVLTAIAIGILLGHYYPELGAQMKPLGDAFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALINGLIIVNVVQPGAGMNVDPATLDAQAVAVYAAQAKEQGIIAFLMDVIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICFYITCILFVVVVLGT
IARVTGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WVKELDHQKLDDVLNNRAPDGKTHEISS

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5032093, Length=401, Percent_Identity=28.6783042394015, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI169790839, Length=475, Percent_Identity=26.5263157894737, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI21314632, Length=441, Percent_Identity=26.984126984127, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI223468566, Length=300, Percent_Identity=30.3333333333333, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI223468564, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI40254478, Length=466, Percent_Identity=25.1072961373391, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4827012, Length=386, Percent_Identity=27.4611398963731, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194239697, Length=466, Percent_Identity=23.6051502145923, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI66773030, Length=413, Percent_Identity=25.6658595641646, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI262359914, Length=415, Percent_Identity=26.0240963855422, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI301601644, Length=188, Percent_Identity=29.7872340425532, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1789947, Length=428, Percent_Identity=94.6261682242991, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=414, Percent_Identity=40.0966183574879, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788024, Length=397, Percent_Identity=21.6624685138539, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789473, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=391, Percent_Identity=26.5984654731458, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=439, Percent_Identity=24.8291571753986, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=439, Percent_Identity=24.8291571753986, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=451, Percent_Identity=24.6119733924612, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17541374, Length=441, Percent_Identity=24.4897959183673, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193206654, Length=462, Percent_Identity=22.7272727272727, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI193206505, Length=452, Percent_Identity=23.2300884955752, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24583025, Length=425, Percent_Identity=22.3529411764706, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137668, Length=425, Percent_Identity=22.3529411764706, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583023, Length=425, Percent_Identity=22.3529411764706, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281360483, Length=457, Percent_Identity=24.2888402625821, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17137666, Length=457, Percent_Identity=24.2888402625821, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281360481, Length=457, Percent_Identity=24.2888402625821, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45451; Mature: 45451

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSLFKSLYFQVLTAIAIGILLGHYYPELGAQMKPLGDAFVKLIKMIIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEIVSTIALINGLIIVNVVQPGAGMNVDPATLDAQAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCHHHHHHH
AAQAKEQGIIAFLMDVIPGSVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHHHHCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICFYITCILFVVVVLGT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARVTGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWVKELDHQKLDDVLNNRAPD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GKTHEISS
CCCCCCCC
>Mature Secondary Structure
MKTSLFKSLYFQVLTAIAIGILLGHYYPELGAQMKPLGDAFVKLIKMIIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEIVSTIALINGLIIVNVVQPGAGMNVDPATLDAQAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCHHHHHHH
AAQAKEQGIIAFLMDVIPGSVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHHHHCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICFYITCILFVVVVLGT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARVTGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSHMDIFHQITLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWVKELDHQKLDDVLNNRAPD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GKTHEISS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA