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The map label for this gene is yhjM

Identifier: 29144173

GI number: 29144173

Start: 4029535

End: 4030644

Strand: Direct

Name: yhjM

Synonym: t3900

Alternate gene names: 29144173

Gene position: 4029535-4030644 (Clockwise)

Preceding gene: 29144172

Following gene: 29144174

Centisome position: 84.09

GC content: 56.22

Gene sequence:

>1110_bases
ATGATGACTATGCTGCGCGGATGGATAACGATGATCGTCATGCTGACGGCAATAAATGCGCAGGCAGCGTGTAGCTGGCC
TGCGTGGGAACAGTTCAAGAAAGATTACATTAGCCAGCAGGGACGCGTTATCGATCCGGGCGATGCGCGAAAAATTACCA
CCTCCGAAGGGCAGAGCTACGCCATGTTCTTTGCCCTGGCGGCGAACGATCGACCGGCGTTCGCGCAACTGTTTAACTGG
ACGCAAAACAATCTGGCGCAGGGATCGCTGCGTGAACATCTGCCCGCCTGGCTGTGGGGACAAAAAGATCCCGACACCTG
GTCGGTGCTGGACAGCAACTCCGCGTCCGACGGCGATATCTGGATGGCATGGTCGCTGCTGGAGGCCGGTCGCCTGTGGA
AAGAGACGCGTTATACCGAGGTGGGCACGGCGTTGCTAAAACGCATCGCCCGCGAAGAGGTCGTGAATGTGCCGGGGCTG
GGCTCAATGCTGCTACCTGGCAAAATCGGCTTTGCCGAGGCGAATAGCTGGCGTTTTAACCCAAGCTATCTGCCGCCGCA
GTTGGCGCAATACTTTAGCCGTTTTGGCGCGCCGTGGTCGACGCTACGGGAAACCAATTTGCGGCTTTTGTTGGAAACCT
CGCCGAAAGGTTTCTCGCCGGACTGGGTGCGTTATGAAAGCAAGCAAGGCTGGCAGTTGAAAGCGGAAAAGACGCTGATC
AGTAGCTACGATGCGATTCGCGTCTATTTATGGACGGGAATGATGCATGATGGCGATCCGCAAAAAGCGCGTTTACTGGC
GCGATTTAAACCGATGGCGACGTTAACGATGAAAAACGGCGTTCCACCGGAGAAAGTGGATGTCGTCAGCGGGAATGCGC
AAGGGACGGGGCCGGTCGGGTTTTCCGCCGCTTTACTGCCTTTCCTGCAAAATCGCGGCGCCCAGGCCGTGCAGCGACAG
CGGGTCGCAGACCATTTTCCTGGCAGCGATGCCTATTACAACTATGTGCTGACTCTCTTTGGACAAGGCTGGGATCAGCA
CCGTTTTCGCTTCACCGTCAAAGGTGAATTATTACCTGACTGGGGCCAGGAATGCGTAAGTTCACGTTAA

Upstream 100 bases:

>100_bases
GGTACTGGCGGCCCTCAGTGTGGTATTACTGGCGTGGGTATTGTGGCGTCTGCTACGTATTCTCAGTCGCCGTCGTCTCG
ACCCTGACCATGAGTAACGC

Downstream 100 bases:

>100_bases
GTCTCATGCACGCTTTTTTGCTGGCTGGCGGTCGAAATGCCTTACCTGGCAAAAGGGGCGTTTCTCGGGCATTGTTGGGG
TTATCGCTTGGTATGGCGCT

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MMTMLRGWITMIVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETSPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWTGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRGAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR

Sequences:

>Translated_369_residues
MMTMLRGWITMIVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETSPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWTGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRGAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR
>Mature_369_residues
MMTMLRGWITMIVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSYAMFFALAANDRPAFAQLFNW
TQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDIWMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGL
GSMLLPGKIGFAEANSWRFNPSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETSPKGFSPDWVRYESKQGWQLKAEKTLI
SSYDAIRVYLWTGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVGFSAALLPFLQNRGAQAVQRQ
RVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPDWGQECVSSR

Specific function: Hydrolyzes carboxymethylcellulose

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family

Homologues:

Organism=Escherichia coli, GI1789951, Length=368, Percent_Identity=81.25, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUN_SALTI (Q8Z289)

Other databases:

- EMBL:   AL627281
- EMBL:   AE014613
- RefSeq:   NP_458303.1
- RefSeq:   NP_807515.1
- ProteinModelPortal:   Q8Z289
- GeneID:   1071146
- GeneID:   1250407
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3900
- KEGG:   sty:STY4183
- HOGENOM:   HBG300178
- OMA:   YALFFAL
- ProtClustDB:   PRK11097
- BioCyc:   SENT209261:T3900-MONOMER
- BioCyc:   SENT220341:STY4183-MONOMER
- BRENDA:   3.2.1.4
- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834
- Gene3D:   G3DSA:1.50.10.10
- PRINTS:   PR00735

Pfam domain/function: PF01270 Glyco_hydro_8; SSF48208 Glyco_trans_6hp

EC number: =3.2.1.4

Molecular weight: Translated: 41703; Mature: 41703

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: ACT_SITE 56-56 ACT_SITE 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTMLRGWITMIVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSY
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCEE
AMFFALAANDRPAFAQLFNWTQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDI
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCEEEECCCCCCCCCE
WMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGLGSMLLPGKIGFAEANSWRFN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETSPKGFSPDWVRYESKQGWQLKAEKTLI
CCCCCHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCHHEEECCCCCCEEEHHHHHH
SSYDAIRVYLWTGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVG
HCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCC
FSAALLPFLQNRGAQAVQRQRVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPD
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCH
WGQECVSSR
HHHHHHCCC
>Mature Secondary Structure
MMTMLRGWITMIVMLTAINAQAACSWPAWEQFKKDYISQQGRVIDPGDARKITTSEGQSY
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCEE
AMFFALAANDRPAFAQLFNWTQNNLAQGSLREHLPAWLWGQKDPDTWSVLDSNSASDGDI
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCEEEECCCCCCCCCE
WMAWSLLEAGRLWKETRYTEVGTALLKRIAREEVVNVPGLGSMLLPGKIGFAEANSWRFN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PSYLPPQLAQYFSRFGAPWSTLRETNLRLLLETSPKGFSPDWVRYESKQGWQLKAEKTLI
CCCCCHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCHHEEECCCCCCEEEHHHHHH
SSYDAIRVYLWTGMMHDGDPQKARLLARFKPMATLTMKNGVPPEKVDVVSGNAQGTGPVG
HCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCC
FSAALLPFLQNRGAQAVQRQRVADHFPGSDAYYNYVLTLFGQGWDQHRFRFTVKGELLPD
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCH
WGQECVSSR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504