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The map label for this gene is waaJ [H]

Identifier: 29144082

GI number: 29144082

Start: 3921140

End: 3922150

Strand: Direct

Name: waaJ [H]

Synonym: t3802

Alternate gene names: 29144082

Gene position: 3921140-3922150 (Clockwise)

Preceding gene: 29144081

Following gene: 29144083

Centisome position: 81.83

GC content: 34.72

Gene sequence:

>1011_bases
ATGGATTCATTTCCTGAGATAGAGATAGCTGAATATAAAGTTTTTGATGAAAGTAATAATAATGATGATAACGTATTAAA
CATTTCTTATGGCGTCGATGAAAACTATCTTGATGGTGTGGGGGTATCAATCGCTTCCGTTGTATTAAACAATAATATCC
CGCTTGCTTTTCATATTATTTGTGACTCATACTCCCCGTGTTTTGTAAAATATATAGAGCGTTTAGCCGTACAGCATCAC
ATTAAAATTTCTCTTTATCTTATTAAAGTAGAAAGCCTTGAAGTATTGCCCCAAACTAAAGTATGGTCGAGAGCAATGTA
TTTTCGTTTATTTGCTTTCGATTATCTCAGCAAGAAGGTAAACACCTTACTTTATTTGGATGCCGATGTTGTATGTAAAG
GATCTTTGCAAGATCTTCTACAGCTTGATCTGACAGAGAAGATTGCTGCGGTAGTAAAAGATGTTGATTCCATCCAGAAT
AAGGTAAATGAGAGATTAAGCGCTTTTAATTTACAAGGTGGTTATTTTAACTCCGGCGTGGTTTTTGTTAACCTGAAATT
ATGGAAAGAGAATGCCTTAACTGAAAAGGCATTTTTACTTCTGGCAGGTAAAGAAGCTGACTCTTTTAAATATCCCGATC
AGGATGTTTTGAATATTCTCCTACAGGATAAAGTTATTTTTCTACCTCGACCATATAATACCATTTATACTATCAAAAGT
GAGTTGAAAGATAGGTCACATAAAAAATATAGCAATATAATTAATGATAATACTATTTTAATTCATTATACGGGCGCTAC
AAAACCATGGCATGCCTGGGCAAATTATCCTTCAGTTATCTATTATAAAAATGCACGACTGAACTCGCCCTGGAAAGATT
CTCCTGCAAAAGATGCGCGTACCATAGTTGAATTTAAGAAGCGATATAAACATCTTCTCGTGCAGGGGCATTATTTTAAA
GGCCTTCTGGCTGGAAGCGCATATCTTTATCGTAAACTTTTCCACAAATAA

Upstream 100 bases:

>100_bases
CAAAGCATATGTTTAATCAGAAGCATTATACTTCGGGTATAAATTATTATATAGCCTACTTTAAACGTAAACTTCTTGAA
TAAAACCCATAGGTGATGTA

Downstream 100 bases:

>100_bases
TTCCCTTCCCTAAATCTCCATTATGATTATTGAAAAAAAGGTTAAAAACTATACCGTCTTTGTCAAAAAAGACGGTGAAA
AATATATTGAGATATTCAAA

Product: lipopolysaccharide 1,2-glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDRSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDSPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK

Sequences:

>Translated_336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDRSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDSPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK
>Mature_336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDRSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDSPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790056, Length=339, Percent_Identity=58.4070796460177, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790057, Length=326, Percent_Identity=35.5828220858896, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 38732; Mature: 38732

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHII
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEEE
CDSYSPCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKV
ECCCCHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHH
NTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLSAFNLQGGYFNSGV
CEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEECCCE
VFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
EEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEHH
ELKDRSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDSPAKDAR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
TIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKLFHK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHII
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEEE
CDSYSPCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKV
ECCCCHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHH
NTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLSAFNLQGGYFNSGV
CEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEECCCE
VFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
EEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEHH
ELKDRSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDSPAKDAR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
TIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKLFHK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]