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The map label for this gene is waaI [H]

Identifier: 29144081

GI number: 29144081

Start: 3920109

End: 3921122

Strand: Direct

Name: waaI [H]

Synonym: t3801

Alternate gene names: 29144081

Gene position: 3920109-3921122 (Clockwise)

Preceding gene: 29144080

Following gene: 29144082

Centisome position: 81.81

GC content: 37.57

Gene sequence:

>1014_bases
ATGAGCAGAAAATATTTTGAAGAAGAAGTCATTCAACAGACTTTAGATTATAACTATGCACAACATAGTGATGCTAATAA
ATTTAATATAGCTTATGGGATTGATAAAAACTTTCTTTTTGGCTGTGGTGTCTCTATTGCATCGGTTCTCATTGCTAACC
GTGAGAAGGCGTTAGCTTTCCATGTTTTTACCGATTTCTTTGGCCCTGAAGATCAGCAGCGATTTGATGCATTAGCAAAA
CAGTACGCTACGCAGATTGTTGTTTATCTCATTGACTGTGAGCGATTAAAATCGCTACCCAGTACCAAAAACTGGACCTA
TGCAACATACTTTAGATTCATTATTGCCGACTATTTTTCAGATAAAACAGATAGAGTGCTTTATCTGGATGCAGATATTG
CATGTAAGGGAAGTATTCAGGAACTTATTGATCTTAATTTCGCTGAAAATGAGATTGCGGCGGTTGTTGCTGAAGGCGAG
TTGGAGTGGTGGACTAAGCGCTCGGTTAGCCTGGCAACGCCTGGGTTGGTTTCTGGCTATTTTAATGCCGGTTTTATTTT
AATTAACATACCTCTTTGGACCGCAGAAAATATCTCTAAGAAAGCGATTGAAATGCTAAAAGATCCGGAGGTAGTACAGC
GCATAACGCACCTTGATCAGGATGTATTAAATATATTGTTAGTGAATAAAGCGCGTTTTGTTGATAAAAAATTTAATACG
CAATTTAGTCTTAACTATGAATTAAAAGATTCAGTTATTAATCCAGTTGATGCTGAGACTGTATTTGTTCATTATATCGG
ACCAACGAAGCCCTGGCATAGTTGGGGGGCTTACCCCGTGTCACAATATTTTTTACGGGCTAAGAGCAACTCACCATGGT
CTCATTGTGCACTTTTAAATCCAGTCACTAGCCATCAGTTACGTTATGCGGCAAAGCATATGTTTAATCAGAAGCATTAT
ACTTCGGGTATAAATTATTATATAGCCTACTTTAAACGTAAACTTCTTGAATAA

Upstream 100 bases:

>100_bases
AGCATGATATTATTCCCGGCACGATTGAGCGATTTTACGATGTGCTATATTTTAAAAATTTTAATAATGCGATATTCTCG
AAATTACAAAAGTGATCACT

Downstream 100 bases:

>100_bases
AACCCATAGGTGATGTAATGGATTCATTTCCTGAGATAGAGATAGCTGAATATAAAGTTTTTGATGAAAGTAATAATAAT
GATGATAACGTATTAAACAT

Product: lipopolysaccharide 1,3-galactosyltransferase

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSRKYFEEEVIQQTLDYNYAQHSDANKFNIAYGIDKNFLFGCGVSIASVLIANREKALAFHVFTDFFGPEDQQRFDALAK
QYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGE
LEWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLRAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHY
TSGINYYIAYFKRKLLE

Sequences:

>Translated_337_residues
MSRKYFEEEVIQQTLDYNYAQHSDANKFNIAYGIDKNFLFGCGVSIASVLIANREKALAFHVFTDFFGPEDQQRFDALAK
QYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGE
LEWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLRAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHY
TSGINYYIAYFKRKLLE
>Mature_336_residues
SRKYFEEEVIQQTLDYNYAQHSDANKFNIAYGIDKNFLFGCGVSIASVLIANREKALAFHVFTDFFGPEDQQRFDALAKQ
YATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGEL
EWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNTQ
FSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLRAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHYT
SGINYYIAYFKRKLLE

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=337, Percent_Identity=54.8961424332344, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1790056, Length=324, Percent_Identity=34.5679012345679, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 38896; Mature: 38764

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKYFEEEVIQQTLDYNYAQHSDANKFNIAYGIDKNFLFGCGVSIASVLIANREKALAF
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCHHHHHHHHHCCCHHEEE
HVFTDFFGPEDQQRFDALAKQYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFS
EEHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHC
DKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGELEWWTKRSVSLATPGLVSGY
CCCCEEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHC
FNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
CCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLRAKSNSPWSHCALLN
EEEEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHEEECC
PVTSHQLRYAAKHMFNQKHYTSGINYYIAYFKRKLLE
CHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRKYFEEEVIQQTLDYNYAQHSDANKFNIAYGIDKNFLFGCGVSIASVLIANREKALAF
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCHHHHHHHHHCCCHHEEE
HVFTDFFGPEDQQRFDALAKQYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFS
EEHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHC
DKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGELEWWTKRSVSLATPGLVSGY
CCCCEEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHC
FNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
CCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLRAKSNSPWSHCALLN
EEEEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHEEECC
PVTSHQLRYAAKHMFNQKHYTSGINYYIAYFKRKLLE
CHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]