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The map label for this gene is levE [H]

Identifier: 29144035

GI number: 29144035

Start: 3862395

End: 3862880

Strand: Direct

Name: levE [H]

Synonym: t3747

Alternate gene names: 29144035

Gene position: 3862395-3862880 (Clockwise)

Preceding gene: 29144034

Following gene: 29144036

Centisome position: 80.6

GC content: 51.65

Gene sequence:

>486_bases
ATGGCCAATATCGTTTTATGCCGCATCGACAGCCGTCTCATTCATGGACAGGTTGTCACGAAGTGGGTCGGACAATCTCA
GGCGAACCGGATAGCCGTTGTGAGTGATGAACTGGACGCCGATCCGTTTATGAAAAATATCTACCTGATGGCGGCGCCGC
CAAATATCAAAGTGGATTGCTTCGGCAACCAGAGTTTTGCCGCTGCATGGAAAGAAAATCAGCTTGGCGACGGTAACGTG
CTGGTGCTTTTTCCCTCACTGGCGGCGGTGCAGGACGCCATACAATTGGGCTTTGACGTCACCCGCATTCAGGTTGGCGG
TCTCGGCGGCGGGCCAAACCGCAAGGCGGTTTTTCAGAATATTACGCTGGATGAAAAAGACGTCGGCATTCTTAACGACC
TGAAAAACCGTGGCGTTCAGGTATTTTTCCAGACCATTCCGGAAGATAAGCCGCAGCCGCTCGACGATATCCTGAAAAAA
TTCTAA

Upstream 100 bases:

>100_bases
ACCGCCTGCGTGGATGAGTTAGTTGAGGCCGGGCGCAGTAGCTGCCTGGATGTGGTCGCCCATGTACGTCAGCTACAACA
ATCACTCTAAGGAAAACATC

Downstream 100 bases:

>100_bases
CCCTTTAATAGACACAAAATCATTCTGGATGCATCGAGGCGGCAACTGAGTGAATCCCCTGGAGCTTACTAAAGTAAGTG
ACTGGGGTGAGCGAAGGCAG

Product: PTS system protein

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFMKNIYLMAAPPNIKVDCFGNQSFAAAWKENQLGDGNV
LVLFPSLAAVQDAIQLGFDVTRIQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKNRGVQVFFQTIPEDKPQPLDDILKK
F

Sequences:

>Translated_161_residues
MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFMKNIYLMAAPPNIKVDCFGNQSFAAAWKENQLGDGNV
LVLFPSLAAVQDAIQLGFDVTRIQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKNRGVQVFFQTIPEDKPQPLDDILKK
F
>Mature_160_residues
ANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFMKNIYLMAAPPNIKVDCFGNQSFAAAWKENQLGDGNVL
VLFPSLAAVQDAIQLGFDVTRIQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKNRGVQVFFQTIPEDKPQPLDDILKKF

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=158, Percent_Identity=29.746835443038, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI87082217, Length=164, Percent_Identity=32.9268292682927, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1789527, Length=158, Percent_Identity=27.8481012658228, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17679; Mature: 17547

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFMKNIYLMAAPPNIKVDC
CCCEEEEEECCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHEEEEEECCCCEEEEE
FGNQSFAAAWKENQLGDGNVLVLFPSLAAVQDAIQLGFDVTRIQVGGLGGGPNRKAVFQN
ECCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEE
ITLDEKDVGILNDLKNRGVQVFFQTIPEDKPQPLDDILKKF
CCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHCC
>Mature Secondary Structure 
ANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFMKNIYLMAAPPNIKVDC
CCEEEEEECCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHEEEEEECCCCEEEEE
FGNQSFAAAWKENQLGDGNVLVLFPSLAAVQDAIQLGFDVTRIQVGGLGGGPNRKAVFQN
ECCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEE
ITLDEKDVGILNDLKNRGVQVFFQTIPEDKPQPLDDILKKF
CCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]