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The map label for this gene is glnG [H]

Identifier: 29143915

GI number: 29143915

Start: 3716723

End: 3718132

Strand: Direct

Name: glnG [H]

Synonym: t3616

Alternate gene names: 29143915

Gene position: 3716723-3718132 (Clockwise)

Preceding gene: 29143914

Following gene: 29143919

Centisome position: 77.56

GC content: 56.1

Gene sequence:

>1410_bases
ATGCAACGAGGAATAGTCTGGGTCGTCGATGATGATAGTTCCATCCGTTGGGTGCTTGAACGTGCTCTCGCCGGGGCAGG
GTTGACCTGTATCACCTTTGAGAATGGTAATGAAGTACTGGCTGCGCTGGCCAGTAAAACGCCGGACGTCTTGCTGTCGG
ATATCCGAATGCCAGGAATGGACGGGCTGGCGCTATTAAAGCAGATTAAACAACGTCATCCGATGCTTCCGGTCATCATT
ATGACCGCGCATTCCGATTTAGATGCCGCCGTGAGCGCTTATCAGCAGGGGGCGTTTGATTATCTGCCTAAACCGTTTGA
TATTGATGAAGCCGTCGCTCTGGTCGAGCGAGCGATTAGCCACTATCAGGAGCAGCAGCAGCCGCGTAATATCGAGGTTA
ACGGCCCGACAACAGACATGATAGGCGAAGCGCCCGCCATGCAGGATGTGTTTCGCATTATTGGTCGGCTGTCGCGTTCT
TCGATTAGCGTGCTGATTAACGGCGAATCAGGAACCGGTAAAGAACTGGTCGCCCATGCGCTACATCGCCATAGCCCACG
CGCCAAAGCGCCGTTTATCGCGCTCAATATGGCGGCCATCCCGAAAGATTTGATTGAATCCGAATTGTTTGGCCATGAGA
AAGGCGCTTTTACCGGGGCGAATACCATCCGGCAGGGCCGTTTCGAGCAGGCTGACGGCGGTACGCTGTTTCTGGACGAA
ATTGGCGATATGCCGCTGGATGTCCAGACTCGTCTGTTACGCGTGCTGGCTGACGGACAGTTCTACCGTGTGGGCGGGTA
CGCGCCGGTAAAAGTGGATGTGCGCATTATTGCCGCCACCCACCAGAACCTCGAACGACGCGTTCAGGAAGGGAAATTCC
GTGAAGACCTGTTCCACCGCCTGAACGTGATTCGTATCCATCTCCCGCCGTTGCGCGAGCGCCGTGAGGATATTCCGCGC
CTGGCGCGCCATTTTTTGCAGGTCGCCGCCCGTGAATTAGGCGTGGAAGCCAAATTATTACATCCGGAAACCGAAACGGC
GTTAACGCGCCTGGCATGGCCTGGTAACGTGCGTCAGTTAGAAAATACCTGCCGCTGGCTCACCGTCATGGCCGCCGGAC
AGGAGGTATTGACCCAGGATTTACCGGGCGAACTGTTTGAAGCCTCGGCGCCGGATAGCCCGTCCCACCTGCCGCCGGAT
AGCTGGGCTACATTACTGGCGCAATGGGCGGATCGCGCCTTGCGATCCGGTCATCAAAACCTGCTTTCTGAAGCGCAGCC
AGAACTGGAAAGAACGCTACTGACCACGGCGTTACGCCATACGCAGGGTCATAAACAGGAAGCCGCCAAGCTGCTCGGTT
GGGGTCGAAACACCCTAACGCGGAAGTTGAAAGAGCTGGGAATGGAGTAA

Upstream 100 bases:

>100_bases
GCAATCTTATCGATCAACACGCCGGCAAAATTGAATTTACCAGTTGGCCGGGGCATACCGAGTTTTCGGTTTACCTGCCG
ATTCGGAAATAGAGGTGTTT

Downstream 100 bases:

>100_bases
ACATTATCCGCGAGTGTAAAGATTAATAATTGAGCGCAAAACGCTGCTATTTTGCGCTTTACTGTTCCGATGAGTTCAGT
ATGATCATGCCCGGCAAACG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCITFENGNEVLAALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHRLNVIRIHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPGELFEASAPDSPSHLPPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAKLLGWGRNTLTRKLKELGME

Sequences:

>Translated_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCITFENGNEVLAALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHRLNVIRIHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPGELFEASAPDSPSHLPPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAKLLGWGRNTLTRKLKELGME
>Mature_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCITFENGNEVLAALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHRLNVIRIHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPGELFEASAPDSPSHLPPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAKLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=95.5223880597015, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=42.7350427350427, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=43.5064935064935, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=37.0689655172414, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=41.4835164835165, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=43.3734939759036, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=365, Percent_Identity=40.8219178082192, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1786524, Length=345, Percent_Identity=43.4782608695652, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.785498489426, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=336, Percent_Identity=40.4761904761905, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=39.2749244712991, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=32.6984126984127, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=230, Percent_Identity=36.9565217391304, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790860, Length=142, Percent_Identity=32.3943661971831, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=31.7073170731707, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=32, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=32.4324324324324, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1786599, Length=149, Percent_Identity=28.8590604026846, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52214; Mature: 52214

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCITFENGNEVLAALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRIHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQL
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLTQDLPGELFEASAPDSPSHLPPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAAKLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCITFENGNEVLAALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRIHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQL
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLTQDLPGELFEASAPDSPSHLPPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAAKLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]