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The map label for this gene is pin [H]

Identifier: 29143757

GI number: 29143757

Start: 3526780

End: 3527346

Strand: Direct

Name: pin [H]

Synonym: t3437

Alternate gene names: 29143757

Gene position: 3526780-3527346 (Clockwise)

Preceding gene: 29143755

Following gene: 29143758

Centisome position: 73.6

GC content: 51.32

Gene sequence:

>567_bases
ATGCTTATTGGCTATGTACGCGTGTCAACAAATGACCAGAACACCGATTTGCAACGCAATGCACTGAGCTGTGCGGGATG
TGAGCGGATTTTTGAGGATAAAATCAGTGGCACCAAGTCCGACAGACCGGGGCTGAAAAAGCTGCTCAGGACACTATCGG
CAGGAGACACGCTGGTTGTCTGGAAGCTGGACAGACTGGGGCGCAGTATGCGGCATCTTGTTACGCTGATAGAAGAGCTG
CGCCAGCGTGGCGTGAATTTCCGAAGCCTGACTGACAGTATTGATACCAGCACCCCAATGGGCCGTTTCTTTTTTCATGT
CATGGGTGCCCTGGCTGAAATGGAACGCGAACTGATAGTTGAACGTACCAGGGCGGGGCTGGCTGCAGCTCGTGCTAAAG
GCAGAGTAGGTGGACGCCGTCCTAAGTTGACCAGCGAACAGTGGGCACAGATTGGGCGTTTACTCGAGGCCGGAGAATCA
AGACAGCGTATTGCACTGATTTTTGATGTAGGCGTTTCTACCATTTATCGAAAATTTCCGGCAAATAAGAACAATAAATC
TCCCTGA

Upstream 100 bases:

>100_bases
CCACATTCCGTTTTGCCGCTTCTCCCAAACCAACGTTTATGAAAATGCAGAGATAACGGCTAACTGGCATCATCTCCGGT
TTTTATTCAGGGGGATGATC

Downstream 100 bases:

>100_bases
ATCAGCTTTATTTTGATTATCCCTGAAAGCAGACAAATACCGTCATTTTGTGTGAATAACGGTACAACTGCGCTTAGCTG
TTTGTCAGGCACAATCACTT

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MLIGYVRVSTNDQNTDLQRNALSCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEEL
RQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGES
RQRIALIFDVGVSTIYRKFPANKNNKSP

Sequences:

>Translated_188_residues
MLIGYVRVSTNDQNTDLQRNALSCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEEL
RQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGES
RQRIALIFDVGVSTIYRKFPANKNNKSP
>Mature_188_residues
MLIGYVRVSTNDQNTDLQRNALSCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEEL
RQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGES
RQRIALIFDVGVSTIYRKFPANKNNKSP

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=86.1878453038674, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=37.7049180327869, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=37.7049180327869, Blast_Score=113, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21119; Mature: 21119

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYVRVSTNDQNTDLQRNALSCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGESRQRIALIFDVGVSTIYRKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC
ANKNNKSP
CCCCCCCC
>Mature Secondary Structure
MLIGYVRVSTNDQNTDLQRNALSCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGESRQRIALIFDVGVSTIYRKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC
ANKNNKSP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]