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The map label for this gene is wecB

Identifier: 29143699

GI number: 29143699

Start: 3476867

End: 3477997

Strand: Reverse

Name: wecB

Synonym: t3377

Alternate gene names: 29143699

Gene position: 3477997-3476867 (Counterclockwise)

Preceding gene: 29143700

Following gene: 29143698

Centisome position: 72.58

GC content: 52.08

Gene sequence:

>1131_bases
GTGAAAGTACTGACTGTATTTGGTACACGACCGGAGGCCATTAAGATGGCGCCTCTGGTCCATGCGCTGGAAAAAGATCC
TCATTTCGAGGCCAAAGTATGCGTTACTGCGCAGCATCGGGAGATGCTTGATCAGGTATTAACTCTTTTTTCTATTGTGC
CTGATTACGATCTCAACATTATGCAGCCGGGACAGGGGTTGACGGAAATTACCTGCCGAATTCTGGAGAGATTAAAGCCG
ATCCTGGCAGATTTTAAACCTGATGTGGTGCTGGTTCATGGAGATACCACCACTACGATTGCTACCAGCCTGGCGGCTTT
CTATCAACGAATTCCGGTAGGGCACGTAGAAGCCGGGTTGCGAACCGGCGATCTCTATTCCCCCTGGCCGGAAGAGGCAA
ACCGCACGCTGACCGGACATCTGGCGATGTACCACTTTGCGCCGACGGAAAACTCGCGGCAGAATCTGTTGCGGGAGAAC
ATTCCGGATGAGCGCATTTTTGTCACTGGCAATACCGTTATTGACGCGCTGATCTGGGTACGCGATCGCGTTCTGACGAG
CGATACATTACAGGCTGAACTGGCCGAGCAGTATCCTTTCCTCAACGCGAATAAGAAGATGATTTTGGTGACCGGTCATC
GGCGTGAAAGCTTTGGTCAGGGCTTTGAGCATATCTGCCAGGCGCTGGCGGAAATTGCCGCCGCGAACCAGAATGTGCAA
ATTGTCTATCCGGTGCATCTCAATCCCAACGTCAGCGAACCTGTCAACCGTATCCTCGGCCATGTTGAAAACGTGGTACT
GATTGAGCCGCAGGACTACTTACCTTTTGTCTGGCTGATGAATCACGCCTGGCTCATTCTGACCGATTCCGGCGGTATTC
AGGAAGAAGCGCCGTCATTAGGTAAGCCGGTATTGGTCATGCGTGAAACCACAGAGCGTCCGGAAGCCATTACCGCTGGT
ACGGTAAGACTGATCGGCACGGACTCCCGGCGCATTGTGGCGGAAGTTATGCGTCTGCTGCACGACGAAAATGAATATCA
AACCATGAGCCGCGCCCATAATCCTTATGGTGACGGGCAATCATGCGCGCGCATTTTACAGGCGTTAAAAAGTTATCGGG
TATCGTTATGA

Upstream 100 bases:

>100_bases
CTAATCGGTGCGGGCGTTGCCTTAACCCGTCGCCGCACGATTTAGCAACACCTCTGCAGTAGGGGGCTGGGCCCCTACTG
TCTATTCGAAGAGAATCGAT

Downstream 100 bases:

>100_bases
GTTTTACGACCATTTCTGTTATTGGGCTGGGGTATATCGGATTACCGACTGCGGCCGCTTTTGCTTCCCGCCAGAAGCAG
GTTATCGGCGTGGATATCAA

Product: UDP-N-acetyl-D-glucosamine 2-epimerase

Products: NA

Alternate protein names: UDP-GlcNAc-2-epimerase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKVLTVFGTRPEAIKMAPLVHALEKDPHFEAKVCVTAQHREMLDQVLTLFSIVPDYDLNIMQPGQGLTEITCRILERLKP
ILADFKPDVVLVHGDTTTTIATSLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFAPTENSRQNLLREN
IPDERIFVTGNTVIDALIWVRDRVLTSDTLQAELAEQYPFLNANKKMILVTGHRRESFGQGFEHICQALAEIAAANQNVQ
IVYPVHLNPNVSEPVNRILGHVENVVLIEPQDYLPFVWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRETTERPEAITAG
TVRLIGTDSRRIVAEVMRLLHDENEYQTMSRAHNPYGDGQSCARILQALKSYRVSL

Sequences:

>Translated_376_residues
MKVLTVFGTRPEAIKMAPLVHALEKDPHFEAKVCVTAQHREMLDQVLTLFSIVPDYDLNIMQPGQGLTEITCRILERLKP
ILADFKPDVVLVHGDTTTTIATSLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFAPTENSRQNLLREN
IPDERIFVTGNTVIDALIWVRDRVLTSDTLQAELAEQYPFLNANKKMILVTGHRRESFGQGFEHICQALAEIAAANQNVQ
IVYPVHLNPNVSEPVNRILGHVENVVLIEPQDYLPFVWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRETTERPEAITAG
TVRLIGTDSRRIVAEVMRLLHDENEYQTMSRAHNPYGDGQSCARILQALKSYRVSL
>Mature_376_residues
MKVLTVFGTRPEAIKMAPLVHALEKDPHFEAKVCVTAQHREMLDQVLTLFSIVPDYDLNIMQPGQGLTEITCRILERLKP
ILADFKPDVVLVHGDTTTTIATSLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFAPTENSRQNLLREN
IPDERIFVTGNTVIDALIWVRDRVLTSDTLQAELAEQYPFLNANKKMILVTGHRRESFGQGFEHICQALAEIAAANQNVQ
IVYPVHLNPNVSEPVNRILGHVENVVLIEPQDYLPFVWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRETTERPEAITAG
TVRLIGTDSRRIVAEVMRLLHDENEYQTMSRAHNPYGDGQSCARILQALKSYRVSL

Specific function: Catalyzes the reversible epimerization at C-2 of UDP-N- acetylglucosamine (UDP-GlcNAc) and thereby provides bacteria with UDP-N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc residues

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family

Homologues:

Organism=Homo sapiens, GI190014632, Length=404, Percent_Identity=22.2772277227723, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI298566312, Length=404, Percent_Identity=22.2772277227723, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4885285, Length=404, Percent_Identity=22.2772277227723, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994967, Length=376, Percent_Identity=85.6382978723404, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECB_SALTI (Q8Z388)

Other databases:

- EMBL:   AL627279
- EMBL:   AE014613
- RefSeq:   NP_457827.1
- RefSeq:   NP_807041.1
- ProteinModelPortal:   Q8Z388
- SMR:   Q8Z388
- GeneID:   1068341
- GeneID:   1249890
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3377
- KEGG:   sty:STY3635
- HOGENOM:   HBG405561
- OMA:   YYHQIPV
- ProtClustDB:   CLSK894436
- BioCyc:   SENT209261:T3377-MONOMER
- BioCyc:   SENT220341:STY3635-MONOMER
- BRENDA:   5.1.3.14
- GO:   GO:0005737
- InterPro:   IPR003331
- PANTHER:   PTHR18964:SF2
- TIGRFAMs:   TIGR00236

Pfam domain/function: PF02350 Epimerase_2

EC number: =5.1.3.14

Molecular weight: Translated: 42265; Mature: 42265

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: ACT_SITE 213-213

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLTVFGTRPEAIKMAPLVHALEKDPHFEAKVCVTAQHREMLDQVLTLFSIVPDYDLNI
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEE
MQPGQGLTEITCRILERLKPILADFKPDVVLVHGDTTTTIATSLAAFYQRIPVGHVEAGL
ECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHCCC
RTGDLYSPWPEEANRTLTGHLAMYHFAPTENSRQNLLRENIPDERIFVTGNTVIDALIWV
CCCCCCCCCHHHHCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
RDRVLTSDTLQAELAEQYPFLNANKKMILVTGHRRESFGQGFEHICQALAEIAAANQNVQ
HHHHHCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEE
IVYPVHLNPNVSEPVNRILGHVENVVLIEPQDYLPFVWLMNHAWLILTDSGGIQEEAPSL
EEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCHHEECCCCEEEEECCCCCCCCCCCC
GKPVLVMRETTERPEAITAGTVRLIGTDSRRIVAEVMRLLHDENEYQTMSRAHNPYGDGQ
CCCEEEEECCCCCCCEEECCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHH
SCARILQALKSYRVSL
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVLTVFGTRPEAIKMAPLVHALEKDPHFEAKVCVTAQHREMLDQVLTLFSIVPDYDLNI
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEE
MQPGQGLTEITCRILERLKPILADFKPDVVLVHGDTTTTIATSLAAFYQRIPVGHVEAGL
ECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHCCC
RTGDLYSPWPEEANRTLTGHLAMYHFAPTENSRQNLLRENIPDERIFVTGNTVIDALIWV
CCCCCCCCCHHHHCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
RDRVLTSDTLQAELAEQYPFLNANKKMILVTGHRRESFGQGFEHICQALAEIAAANQNVQ
HHHHHCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEE
IVYPVHLNPNVSEPVNRILGHVENVVLIEPQDYLPFVWLMNHAWLILTDSGGIQEEAPSL
EEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCHHEECCCCEEEEECCCCCCCCCCCC
GKPVLVMRETTERPEAITAGTVRLIGTDSRRIVAEVMRLLHDENEYQTMSRAHNPYGDGQ
CCCEEEEECCCCCCCEEECCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHH
SCARILQALKSYRVSL
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504