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The map label for this gene is rffH [H]

Identifier: 29143696

GI number: 29143696

Start: 3473627

End: 3474508

Strand: Reverse

Name: rffH [H]

Synonym: t3374

Alternate gene names: 29143696

Gene position: 3474508-3473627 (Counterclockwise)

Preceding gene: 29143697

Following gene: 29143695

Centisome position: 72.51

GC content: 55.22

Gene sequence:

>882_bases
ATGAAAGGCATCATTCTGGCGGGAGGTTCCGGTACCCGGTTGCATCCCATTACGCGCGGCGTATCAAAGCAGCTTCTGCC
CGTCTACGATAAACCGATGATTTACTATCCGTTATCGGTGCTAATGCTGGCGGGCATTCGTGAGATCTTGATCATCACCA
CGCCGGAGGATAAACGCGACTTTCAGCGTTTGTTGGGCGACGGCGCAGCATTCGGTATCGATTTACATTATGCCGAGCAG
CCCAGTCCGGACGGGCTGGCGCAGGCCTTTATCATTGGCGAAACGTTCCTCAACGGTGAACCCTCATGCCTAGTATTGGG
CGACAATATCTTTTTTGGTCAGGGCTTTAGCCCCAAACTCCGTCAGGTCGCGGCGCGTACCGAAGGCGCCACAGTATTTG
GCTATCAGGTCATGGACCCGGAACGCTTTGGCGTCGTGGAGTTTGACGATGATTTTCGCGCGCTCTCGCTGGAAGAAAAG
CCGAAACAGCCTAAATCTAACTGGGCGGTCACCGGCCTCTATTTCTATGACAGCAAGGTCGTCGAGTATGCGAAGCGCGT
GAAACCGTCCGGGCGTGGCGAGCTGGAGATCACTTCTATCAACCAGATGTATCTGGAGGAAGGTAAGCTGACCGTTGAAC
TGCTGGGCCGCGGCTTCGCCTGGTTGGATACGGGCACCCACGACAGTCTGATTGAGGCGAGCACTTTTGTGCAGACGGTG
GAAAAGCGCCAGGGCTTCAAAATTGCCTGTCTGGAGGAGATTGCCTGGCGCAACGGCTGGCTGGATGATGATGGTTTGAA
ACGCGCCGCCAGTCAGCTTGAAAAAACCGGCTATGGCCAATATTTACTGGAGTTGCTCCGTGCCCGTCCGCGCCAGTATT
GA

Upstream 100 bases:

>100_bases
ATGAGGCCTGGTGGAAACCCGTGCAGGATGGCAGTTATCAGGGCGAACGCTTAGGTCTGAAACGCTAAACGGCAGGCTCT
TACTCCTGGAGGAGAAACGA

Downstream 100 bases:

>100_bases
ACCGTTACACTGGGAAAATACGTTCTTTGGCGTGAATAGCGGCATTGTGCGTATCGATGCCTCAGCGCCCGAATTAACGC
CAGAAGCGCTGCAGGCATGG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKGIILAGGSGTRLHPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIREILIITTPEDKRDFQRLLGDGAAFGIDLHYAEQ
PSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKLRQVAARTEGATVFGYQVMDPERFGVVEFDDDFRALSLEEK
PKQPKSNWAVTGLYFYDSKVVEYAKRVKPSGRGELEITSINQMYLEEGKLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGLKRAASQLEKTGYGQYLLELLRARPRQY

Sequences:

>Translated_293_residues
MKGIILAGGSGTRLHPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIREILIITTPEDKRDFQRLLGDGAAFGIDLHYAEQ
PSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKLRQVAARTEGATVFGYQVMDPERFGVVEFDDDFRALSLEEK
PKQPKSNWAVTGLYFYDSKVVEYAKRVKPSGRGELEITSINQMYLEEGKLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGLKRAASQLEKTGYGQYLLELLRARPRQY
>Mature_293_residues
MKGIILAGGSGTRLHPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIREILIITTPEDKRDFQRLLGDGAAFGIDLHYAEQ
PSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKLRQVAARTEGATVFGYQVMDPERFGVVEFDDDFRALSLEEK
PKQPKSNWAVTGLYFYDSKVVEYAKRVKPSGRGELEITSINQMYLEEGKLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGLKRAASQLEKTGYGQYLLELLRARPRQY

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=253, Percent_Identity=25.296442687747, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI11761621, Length=253, Percent_Identity=25.296442687747, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790224, Length=293, Percent_Identity=93.8566552901024, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=65.1567944250871, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787488, Length=248, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788355, Length=236, Percent_Identity=23.3050847457627, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=229, Percent_Identity=26.2008733624454, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=258, Percent_Identity=26.7441860465116, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355443, Length=252, Percent_Identity=24.6031746031746, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24644084, Length=252, Percent_Identity=24.6031746031746, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32812; Mature: 32812

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLHPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIREILIITTPEDKRD
CCCEEEECCCCCEECHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCHHH
FQRLLGDGAAFGIDLHYAEQPSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKL
HHHHHCCCCEEEEEEEECCCCCCCHHHHEEEEEHHHCCCCCCEEEEECCEEECCCCCHHH
RQVAARTEGATVFGYQVMDPERFGVVEFDDDFRALSLEEKPKQPKSNWAVTGLYFYDSKV
HHHHHHCCCCEEEEEEEECCCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECHHH
VEYAKRVKPSGRGELEITSINQMYLEEGKLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
HHHHHHCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGFKIACLEEIAWRNGWLDDDGLKRAASQLEKTGYGQYLLELLRARPRQY
HHHCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGIILAGGSGTRLHPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIREILIITTPEDKRD
CCCEEEECCCCCEECHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCHHH
FQRLLGDGAAFGIDLHYAEQPSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKL
HHHHHCCCCEEEEEEEECCCCCCCHHHHEEEEEHHHCCCCCCEEEEECCEEECCCCCHHH
RQVAARTEGATVFGYQVMDPERFGVVEFDDDFRALSLEEKPKQPKSNWAVTGLYFYDSKV
HHHHHHCCCCEEEEEEEECCCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECHHH
VEYAKRVKPSGRGELEITSINQMYLEEGKLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
HHHHHHCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGFKIACLEEIAWRNGWLDDDGLKRAASQLEKTGYGQYLLELLRARPRQY
HHHCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]