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The map label for this gene is rffG [H]

Identifier: 29143697

GI number: 29143697

Start: 3474541

End: 3475608

Strand: Reverse

Name: rffG [H]

Synonym: t3375

Alternate gene names: 29143697

Gene position: 3475608-3474541 (Counterclockwise)

Preceding gene: 29143698

Following gene: 29143696

Centisome position: 72.53

GC content: 55.9

Gene sequence:

>1068_bases
ATGAAACGCATTCTGGTGACCGGCGGCGCAGGCTTTATCGGATCTGCGGTGGTGCGGCATATCATCCATGAAACGGCAGA
CGCGGTGGTGGTGGTGGATAAACTCACCTATGCGGGCAACCTGATGTCTCTGGCGTCGGTGGCGCAAAGCGACCGTTTCG
CCTTTGAGAAGGTGGATATCTGCGATCGGGCATCACTGGAGCAAGTTTTCCAGCAGTATCAGCCCGATAGCGTGATGCAC
CTGGCGGCGGAAAGCCATGTAGACCGTTCCATCGACGGCCCGGCGGCGTTTATTGAAACGAATATCGTTGGTACTTACAC
CTTGCTGGAAGCCGCTCGCGCTTACTGGTCCGCGCTTGACGCGGACGCTAAAGCGGCGTTCCGCTTCCACCATATCTCCA
CCGATGAAGTGTATGGCGATCTGCATACTGCAGACGATTTCTTCACCGAAACCACGCCATATGCGCCAAGCAGCCCTTAT
TCCGCCTCCAAAGCCAGCAGCGACCATCTGGTACGCGCCTGGTTACGTACCTACGGTCTGCCTACGCTTGTCACCAACTG
CTCTAATAACTACGGGCCCTACCATTTCCCGGAAAAACTGATCCCGCTGATGATTCTGAACGCGCTGGCGGGTAAACCAT
TGCCGGTCTATGGCAACGGTCAGCAAATTCGCGACTGGCTGTATGTGGAGGATCATGCCCGTGCGTTGTATCACGTGGTG
ACGAACGGTGCGGTGGGCGAAACGTATAATATCGGCGGTCATAACGAACGTAAAAATCTGGATGTGGTCAGGACGATCTG
CGCTCTGTTGGAGGAACTGGCCCCGCAAAAACCGCAGGGTGTGGCGAATTATCACGATCTGATTACCTTCGTCGACGATC
GACCCGGCCATGACTTACGTTATGCCATCGACGCGTCGAAAATCGCCCGCGAGCTGGGCTGGACGCCGCAGGAAACCTTC
GAAAGCGGGATGCGAAAAACCGTTCAGTGGTATCTCGCCAATGAGGCCTGGTGGAAACCCGTGCAGGATGGCAGTTATCA
GGGCGAACGCTTAGGTCTGAAACGCTAA

Upstream 100 bases:

>100_bases
ATGTGCTGGTGATGCTGGTCGATCACGACGAATTTAAAGCGATTCCCGGCGATGCGGTTCATCAACGTTATGTGGTTGAT
ACCAAAGGGGTCTGGCGCTG

Downstream 100 bases:

>100_bases
ACGGCAGGCTCTTACTCCTGGAGGAGAAACGAATGAAAGGCATCATTCTGGCGGGAGGTTCCGGTACCCGGTTGCATCCC
ATTACGCGCGGCGTATCAAA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKRILVTGGAGFIGSAVVRHIIHETADAVVVVDKLTYAGNLMSLASVAQSDRFAFEKVDICDRASLEQVFQQYQPDSVMH
LAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWSALDADAKAAFRFHHISTDEVYGDLHTADDFFTETTPYAPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYHVV
TNGAVGETYNIGGHNERKNLDVVRTICALLEELAPQKPQGVANYHDLITFVDDRPGHDLRYAIDASKIARELGWTPQETF
ESGMRKTVQWYLANEAWWKPVQDGSYQGERLGLKR

Sequences:

>Translated_355_residues
MKRILVTGGAGFIGSAVVRHIIHETADAVVVVDKLTYAGNLMSLASVAQSDRFAFEKVDICDRASLEQVFQQYQPDSVMH
LAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWSALDADAKAAFRFHHISTDEVYGDLHTADDFFTETTPYAPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYHVV
TNGAVGETYNIGGHNERKNLDVVRTICALLEELAPQKPQGVANYHDLITFVDDRPGHDLRYAIDASKIARELGWTPQETF
ESGMRKTVQWYLANEAWWKPVQDGSYQGERLGLKR
>Mature_355_residues
MKRILVTGGAGFIGSAVVRHIIHETADAVVVVDKLTYAGNLMSLASVAQSDRFAFEKVDICDRASLEQVFQQYQPDSVMH
LAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWSALDADAKAAFRFHHISTDEVYGDLHTADDFFTETTPYAPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYHVV
TNGAVGETYNIGGHNERKNLDVVRTICALLEELAPQKPQGVANYHDLITFVDDRPGHDLRYAIDASKIARELGWTPQETF
ESGMRKTVQWYLANEAWWKPVQDGSYQGERLGLKR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=33.4302325581395, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=26.2686567164179, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4504031, Length=284, Percent_Identity=24.2957746478873, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI56237023, Length=346, Percent_Identity=26.878612716763, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=346, Percent_Identity=26.878612716763, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=346, Percent_Identity=26.878612716763, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=88.9830508474576, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=73.0878186968839, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=26.2108262108262, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788366, Length=311, Percent_Identity=26.0450160771704, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788365, Length=346, Percent_Identity=23.4104046242775, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=35, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=34.6385542168675, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=356, Percent_Identity=26.4044943820225, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=364, Percent_Identity=25.5494505494505, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=366, Percent_Identity=25.4098360655738, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17539422, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=192, Percent_Identity=27.0833333333333, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=192, Percent_Identity=27.0833333333333, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=196, Percent_Identity=27.0408163265306, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=361, Percent_Identity=26.0387811634349, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=26.1904761904762, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=27.6243093922652, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24158427, Length=339, Percent_Identity=24.4837758112094, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39577; Mature: 39577

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVTGGAGFIGSAVVRHIIHETADAVVVVDKLTYAGNLMSLASVAQSDRFAFEKVDI
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHCH
CDRASLEQVFQQYQPDSVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWSALD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHC
ADAKAAFRFHHISTDEVYGDLHTADDFFTETTPYAPSSPYSASKASSDHLVRAWLRTYGL
CCHHHHEEEEECCHHHHHCCHHCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PTLVTNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYHVV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHH
TNGAVGETYNIGGHNERKNLDVVRTICALLEELAPQKPQGVANYHDLITFVDDRPGHDLR
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCCEE
YAIDASKIARELGWTPQETFESGMRKTVQWYLANEAWWKPVQDGSYQGERLGLKR
EEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRILVTGGAGFIGSAVVRHIIHETADAVVVVDKLTYAGNLMSLASVAQSDRFAFEKVDI
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHCH
CDRASLEQVFQQYQPDSVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWSALD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHC
ADAKAAFRFHHISTDEVYGDLHTADDFFTETTPYAPSSPYSASKASSDHLVRAWLRTYGL
CCHHHHEEEEECCHHHHHCCHHCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PTLVTNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYHVV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHH
TNGAVGETYNIGGHNERKNLDVVRTICALLEELAPQKPQGVANYHDLITFVDDRPGHDLR
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCCEE
YAIDASKIARELGWTPQETFESGMRKTVQWYLANEAWWKPVQDGSYQGERLGLKR
EEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]