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The map label for this gene is rffM [H]

Identifier: 29143690

GI number: 29143690

Start: 3467417

End: 3468157

Strand: Reverse

Name: rffM [H]

Synonym: t3368

Alternate gene names: 29143690

Gene position: 3468157-3467417 (Counterclockwise)

Preceding gene: 29143691

Following gene: 29143689

Centisome position: 72.37

GC content: 55.74

Gene sequence:

>741_bases
ATGACCAATAATGCCGCTGCGCCCCTTTACTCGCTGCGTGGATTACCTCTGATTGGCTGGCGCGACATGTCACATGCGCT
GAATTATCTGTTTGCCGATGGTCAACTGAAGCAAGGCACGTTGGTGGCGATCAACGCCGAAAAGCTGCTGACGGCGGAAG
ATAATCCGGAAGTCCGCGCGTTGATAGCAGCGGCGGAATTTAAATATGCCGATGGCATCAGCGTGGTGCGTTCAATACGT
AAAAAATTTCCGCAGGCGCAGGTTTCTCGCGTGGCTGGCGCCGACCTTTGGGAAGCGTTAATGGCGCGCGCGGGGAAAGA
AGGCACGCCGGTATTTTTTGTCGGCGGCAAGCCTGAGGTGCTGGCGCAGACGGAAGCAAAACTGCGGACGCAGTGGAACG
TCAATATTGTGGGTAGCCAGGACGGTTACTTTACGCCGGAGCAGCGTCAGGCGCTATTTGCGCGTATCCATGCCAGCGGC
GCGAAAATTGTCACCGTCGCGATGGGATCGCCAAAACAGGAATTACTCATGCGCGATTGTCGGGAAGTGCATCCCCATGC
GCTATATATGGGGGTGGGCGGTACCTATGATGTGTTTACCGGTCACGTCAAACGCGCCCCAAAAATATGGCAGAACCTGG
GGCTGGAGTGGTTGTATCGTCTGCTTTCGCAACCGAAGCGTATTACCCGCCAGATGCGTCTGCTGCGCTATCTTCGCTGG
CACTATACTGGCGATCTCTGA

Upstream 100 bases:

>100_bases
GCCTGCTGGTCGCTAAACTTCTGTTCTGGTTATTTGATAGCGCCGGACTGATTCATAAACGTACAACTTCGCTCCCTCAG
GCACAGATGGATGGAAAACT

Downstream 100 bases:

>100_bases
TTCTCCCTTCTGATGACTTGAGCAGCGGACTGTGCTTATGGCTCTGCTCATTTACAACATAATCGATGCTTTCTTACACA
ATAAGCGCATTTTTTTAATG

Product: UDP-N-acetyl-D-mannosaminuronic acid transferase

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTNNAAAPLYSLRGLPLIGWRDMSHALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRALIAAAEFKYADGISVVRSIR
KKFPQAQVSRVAGADLWEALMARAGKEGTPVFFVGGKPEVLAQTEAKLRTQWNVNIVGSQDGYFTPEQRQALFARIHASG
AKIVTVAMGSPKQELLMRDCREVHPHALYMGVGGTYDVFTGHVKRAPKIWQNLGLEWLYRLLSQPKRITRQMRLLRYLRW
HYTGDL

Sequences:

>Translated_246_residues
MTNNAAAPLYSLRGLPLIGWRDMSHALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRALIAAAEFKYADGISVVRSIR
KKFPQAQVSRVAGADLWEALMARAGKEGTPVFFVGGKPEVLAQTEAKLRTQWNVNIVGSQDGYFTPEQRQALFARIHASG
AKIVTVAMGSPKQELLMRDCREVHPHALYMGVGGTYDVFTGHVKRAPKIWQNLGLEWLYRLLSQPKRITRQMRLLRYLRW
HYTGDL
>Mature_245_residues
TNNAAAPLYSLRGLPLIGWRDMSHALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRALIAAAEFKYADGISVVRSIRK
KFPQAQVSRVAGADLWEALMARAGKEGTPVFFVGGKPEVLAQTEAKLRTQWNVNIVGSQDGYFTPEQRQALFARIHASGA
KIVTVAMGSPKQELLMRDCREVHPHALYMGVGGTYDVFTGHVKRAPKIWQNLGLEWLYRLLSQPKRITRQMRLLRYLRWH
YTGDL

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=246, Percent_Identity=87.8048780487805, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 27605; Mature: 27474

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNAAAPLYSLRGLPLIGWRDMSHALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRA
CCCCCCCCHHHHCCCCEECCHHHHHHHHHHHCCCCCCCCEEEEEEHHHEEECCCCCCCEE
LIAAAEFKYADGISVVRSIRKKFPQAQVSRVAGADLWEALMARAGKEGTPVFFVGGKPEV
EEHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCE
LAQTEAKLRTQWNVNIVGSQDGYFTPEQRQALFARIHASGAKIVTVAMGSPKQELLMRDC
EHHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
REVHPHALYMGVGGTYDVFTGHVKRAPKIWQNLGLEWLYRLLSQPKRITRQMRLLRYLRW
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHH
HYTGDL
HCCCCC
>Mature Secondary Structure 
TNNAAAPLYSLRGLPLIGWRDMSHALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRA
CCCCCCCHHHHCCCCEECCHHHHHHHHHHHCCCCCCCCEEEEEEHHHEEECCCCCCCEE
LIAAAEFKYADGISVVRSIRKKFPQAQVSRVAGADLWEALMARAGKEGTPVFFVGGKPEV
EEHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCE
LAQTEAKLRTQWNVNIVGSQDGYFTPEQRQALFARIHASGAKIVTVAMGSPKQELLMRDC
EHHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
REVHPHALYMGVGGTYDVFTGHVKRAPKIWQNLGLEWLYRLLSQPKRITRQMRLLRYLRW
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHH
HYTGDL
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA