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The map label for this gene is yhdJ [H]

Identifier: 29143634

GI number: 29143634

Start: 3395740

End: 3396624

Strand: Direct

Name: yhdJ [H]

Synonym: t3301

Alternate gene names: 29143634

Gene position: 3395740-3396624 (Clockwise)

Preceding gene: 29143633

Following gene: 29143635

Centisome position: 70.86

GC content: 45.65

Gene sequence:

>885_bases
ATGAAAGCGGAATGTGAACCTCAGTACTTTGGCGATGAATCGAAGAAGATTATTCACGGTGACGCGCTAACAGAACTTAA
AAAACTGCCTTCTGAAAGCATTGACTTAATTTTTGCCGATCCGCCTTACAATATCGGAAAAGATTTCGACGGTATGGTGG
AATCCTGGGACGAAGCGTCTTTTCTGGCCTGGCTGTATGAATGCATTGACGAGTGCCACCGCGTACTGAAGAAACACGGC
ACCATGTACATCATGAATAGCACAGAAAATATGCCGTATATCGATCTCAAATGCCGAACGCTTTTTACCATCAAAAGCCG
TATCGTCTGGTCCTATGATAGTTCCGGGGTGCAGGCGAAAAAATACTTTGGTTCTATGTATGAACCGATCCTGATGATGG
TAAAAAACCCGAAAAGCTATACCTTTAACCGTGACGCGATTCTGGTCGAGACCACTACGGGCGCTAAGCGCGCGCTAATC
GATTATCGAAAAAACCCGCCCCAACCATACAATCAGAAAAAAGTGCCGGGCAACGTCTGGTCATTTCCTCGCGTACGTTA
TCTGATGGATGAATATGAAAACCATCCTACGCAAAAGCCCAGCGCGCTGTTAAAACGGATCATACTGGCCTCTTCTAACC
CGAGCGATACCGTATTGGACCCTTTCGCGGGCAGTTTCACCACAGGCGCTGTCGCAGCGGCGTCAGGTCGTAAGTTTATC
GGTATTGAACTCAATAATGAGTACGTAAAAATGGGGCTCAGAAGATTGAGCGTCACTTCGCATTATTCAGAAAATGAACT
TGCAAAAGTCAAAAAGAGAAAAACACAAAACCTGTCCAAAAAGCAGCGAAATGTCGGGATAAACGCGCTATCTTCAGAGA
AGTAA

Upstream 100 bases:

>100_bases
TACGGCATGAACTAATTTCGATTAGCTAACTGCTTGTTTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAC
GCATTACATGGAATTTGGTC

Downstream 100 bases:

>100_bases
AAGCATTGTAAGTCCGCTTTTCAGCGTCAAAATTTCATGTATATTCCCAGCACGCATGGGCATGAATACACAGGATTTGA
CGATGATACGTAAGTATTGG

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKAECEPQYFGDESKKIIHGDALTELKKLPSESIDLIFADPPYNIGKDFDGMVESWDEASFLAWLYECIDECHRVLKKHG
TMYIMNSTENMPYIDLKCRTLFTIKSRIVWSYDSSGVQAKKYFGSMYEPILMMVKNPKSYTFNRDAILVETTTGAKRALI
DYRKNPPQPYNQKKVPGNVWSFPRVRYLMDEYENHPTQKPSALLKRIILASSNPSDTVLDPFAGSFTTGAVAAASGRKFI
GIELNNEYVKMGLRRLSVTSHYSENELAKVKKRKTQNLSKKQRNVGINALSSEK

Sequences:

>Translated_294_residues
MKAECEPQYFGDESKKIIHGDALTELKKLPSESIDLIFADPPYNIGKDFDGMVESWDEASFLAWLYECIDECHRVLKKHG
TMYIMNSTENMPYIDLKCRTLFTIKSRIVWSYDSSGVQAKKYFGSMYEPILMMVKNPKSYTFNRDAILVETTTGAKRALI
DYRKNPPQPYNQKKVPGNVWSFPRVRYLMDEYENHPTQKPSALLKRIILASSNPSDTVLDPFAGSFTTGAVAAASGRKFI
GIELNNEYVKMGLRRLSVTSHYSENELAKVKKRKTQNLSKKQRNVGINALSSEK
>Mature_294_residues
MKAECEPQYFGDESKKIIHGDALTELKKLPSESIDLIFADPPYNIGKDFDGMVESWDEASFLAWLYECIDECHRVLKKHG
TMYIMNSTENMPYIDLKCRTLFTIKSRIVWSYDSSGVQAKKYFGSMYEPILMMVKNPKSYTFNRDAILVETTTGAKRALI
DYRKNPPQPYNQKKVPGNVWSFPRVRYLMDEYENHPTQKPSALLKRIILASSNPSDTVLDPFAGSFTTGAVAAASGRKFI
GIELNNEYVKMGLRRLSVTSHYSENELAKVKKRKTQNLSKKQRNVGINALSSEK

Specific function: Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3') [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=283, Percent_Identity=80.2120141342756, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33555; Mature: 33555

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAECEPQYFGDESKKIIHGDALTELKKLPSESIDLIFADPPYNIGKDFDGMVESWDEAS
CCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHH
FLAWLYECIDECHRVLKKHGTMYIMNSTENMPYIDLKCRTLFTIKSRIVWSYDSSGVQAK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHEEEEEEEEEEEEECCCCCHHH
KYFGSMYEPILMMVKNPKSYTFNRDAILVETTTGAKRALIDYRKNPPQPYNQKKVPGNVW
HHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCC
SFPRVRYLMDEYENHPTQKPSALLKRIILASSNPSDTVLDPFAGSFTTGAVAAASGRKFI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCEEE
GIELNNEYVKMGLRRLSVTSHYSENELAKVKKRKTQNLSKKQRNVGINALSSEK
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAECEPQYFGDESKKIIHGDALTELKKLPSESIDLIFADPPYNIGKDFDGMVESWDEAS
CCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHH
FLAWLYECIDECHRVLKKHGTMYIMNSTENMPYIDLKCRTLFTIKSRIVWSYDSSGVQAK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHEEEEEEEEEEEEECCCCCHHH
KYFGSMYEPILMMVKNPKSYTFNRDAILVETTTGAKRALIDYRKNPPQPYNQKKVPGNVW
HHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCC
SFPRVRYLMDEYENHPTQKPSALLKRIILASSNPSDTVLDPFAGSFTTGAVAAASGRKFI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCEEE
GIELNNEYVKMGLRRLSVTSHYSENELAKVKKRKTQNLSKKQRNVGINALSSEK
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 1459953; 2835774 [H]