Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 29143514

GI number: 29143514

Start: 3270768

End: 3271232

Strand: Direct

Name: gatA [H]

Synonym: t3178

Alternate gene names: 29143514

Gene position: 3270768-3271232 (Clockwise)

Preceding gene: 29143513

Following gene: 29143515

Centisome position: 68.26

GC content: 53.98

Gene sequence:

>465_bases
ATGAGCCAATTATTTGTCCGAACCGGAATCACATTCGACTCGAGCCAACAGGCGCTGGCGCACATTGGCAAAGAGATGCT
GGCGAAAGGCGTGGTACATGATAGTTATCCGCAGGCACTGGTGGAACGTGAAGCGTCCTTTCCCACCGGCATTGCGCTGG
AGCGCCACGCCGTCGCTATCCCTCATTGCGAGGCAGTTCATGCGAAATCACCCGCTATCTATCTGATTCGTCCAGATAAG
CCGGTCATGTTTCAACAGGCGGATGATGACGAAGAGATTGCCGTTTCGTTAATCATCGCCTTAATCGTTGAAAACCCGGC
GGCACAGCTAAAACTTCTGCGCCGTCTGTTCGGGGCATTACAAATCCCGGACACCATAGAAGCCTTACTGAGCGCCCCGG
ATGCCGAACTGGCGTCGTGCTTTGAGCACAAAGTCCTTACGGCTGAGCAGTGCGTCCAGGTTTAA

Upstream 100 bases:

>100_bases
CCCCCACGCCGCATAACCTGATTATTGACAAAATTCAGGATGTTCTGCGCGCTTATCGCTTCGGTTGCACGCCGGATGTC
GCCTGAATAGTGGAGAATTT

Downstream 100 bases:

>100_bases
TAATAAATTGATAATAATGAGGAATACCCTATGAAACGTAAAGTAATTGTGGCCTGTGGCGGTGCGGTTGCAACCTCAAC
GATGGCTGCGGAAGAAATTA

Product: PTS system galactitol-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gat; PTS system galactitol-specific EIIA component [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSQLFVRTGITFDSSQQALAHIGKEMLAKGVVHDSYPQALVEREASFPTGIALERHAVAIPHCEAVHAKSPAIYLIRPDK
PVMFQQADDDEEIAVSLIIALIVENPAAQLKLLRRLFGALQIPDTIEALLSAPDAELASCFEHKVLTAEQCVQV

Sequences:

>Translated_154_residues
MSQLFVRTGITFDSSQQALAHIGKEMLAKGVVHDSYPQALVEREASFPTGIALERHAVAIPHCEAVHAKSPAIYLIRPDK
PVMFQQADDDEEIAVSLIIALIVENPAAQLKLLRRLFGALQIPDTIEALLSAPDAELASCFEHKVLTAEQCVQV
>Mature_153_residues
SQLFVRTGITFDSSQQALAHIGKEMLAKGVVHDSYPQALVEREASFPTGIALERHAVAIPHCEAVHAKSPAIYLIRPDKP
VMFQQADDDEEIAVSLIIALIVENPAAQLKLLRRLFGALQIPDTIEALLSAPDAELASCFEHKVLTAEQCVQV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788410, Length=150, Percent_Identity=63.3333333333333, Blast_Score=204, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16809; Mature: 16677

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLFVRTGITFDSSQQALAHIGKEMLAKGVVHDSYPQALVEREASFPTGIALERHAVAI
CCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECCCEEEC
PHCEAVHAKSPAIYLIRPDKPVMFQQADDDEEIAVSLIIALIVENPAAQLKLLRRLFGAL
CCCHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
QIPDTIEALLSAPDAELASCFEHKVLTAEQCVQV
CCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
SQLFVRTGITFDSSQQALAHIGKEMLAKGVVHDSYPQALVEREASFPTGIALERHAVAI
CCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECCCEEEC
PHCEAVHAKSPAIYLIRPDKPVMFQQADDDEEIAVSLIIALIVENPAAQLKLLRRLFGAL
CCCHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
QIPDTIEALLSAPDAELASCFEHKVLTAEQCVQV
CCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]